Transcriptome Profiling of the Mangrove Plant<i>Bruguiera gymnorhiza</i>and Identification of Salt Tolerance Genes by<i>Agrobacterium</i>Functional Screening

Yamanaka, Takuya; Miyama, Masashi; Tada, Yuichi

doi:10.1271/bbb.80513

Cited by 26 publications

(26 citation statements)

References 31 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yamanaka et al . () found that three salt stress responsive genes encoding lipid transfer, zinc finger and ankyrin repeat proteins from B . gymnorhiza confer salt tolerance when overexpressed in Arabidopsis thaliana .…”

Section: Introductionmentioning

confidence: 99%

“…A molecular biological study of salt tolerance was first reported in a mangrove (Bruguiera gymnorrhiza) by Sugihara et al (2000), and the results showed the importance of oxygen evolving enhancer protein 1 (OEE1) in the salt tolerance. Yamanaka et al (2009) found that three salt stress responsive genes encoding lipid transfer, zinc finger and ankyrin repeat proteins from B. gymnorhiza confer salt tolerance when overexpressed in Arabidopsis thaliana. Recently, comparative proteomics have been conducted to investigate the mechanisms of salt tolerance in the mangrove species of B. gymnorhiza (Tada & Kashimura 2009;Zhu et al 2012) and Kandelia candel (Wang et al 2013a).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A multilevel investigation to discover why Kandelia candel thrives in high salinity

Wang

Pan

et al. 2016

Plant Cell & Environment

View full text Add to dashboard Cite

The halophilic mangrove species Kandelia candel is an excellent model for understanding why halophytes thrive in high salinity. Preliminary transcriptomic analyses revealed that genes involved in diverse functions, such as in phenylpropanoid and amino acid metabolisms, and those in DNA replication and damage repair were highly responsive to salt stress. Proteomic analyses revealed that the proteins involved in light reaction of photosynthesis, amino acid and carbohydrate metabolisms, secondary metabolite biosynthesis and posttranslational modification showed increased levels in response to salt stress. The metabolisms of phenylpropanoids and amino acids under salt stress were systematically examined based on the preliminary omics analyses. The activities of phenylpropanoid biosynthetic enzymes and the contents of phenols, flavonoids, anthocyanins and lignins were significantly increased under salt stress. In the free amino acid pool, glutamate was the most abundant. Together with γ-aminobutyric acid, glutamate levels further increased, while proline levels remained unchanged in response to salt stress. These findings point to the potential importance of phenylpropanoids and free amino acids in salt tolerance of K. candel that have been observed, but not systemically investigated at the levels of gene expression, enzyme activity and metabolite accumulation in glycophytes and non-tree halophytes.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A multilevel investigation to discover why Kandelia candel thrives in high salinity

Wang

Pan

et al. 2016

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…Several studies have addressed mangrove salinity response mechanisms by focusing on the photosynthetic performance at the whole-plant level [18,19], the expression and identification of genes that might contribute to high salinity tolerance [20][21][22], as well as changes in protein abundance of leaves under long term salt stress (12 d and 45 d, respectively) [23,24]. However, except for our initial report examining the K. candel chloroplast proteome at various salt conditions [25], no reports exist that focus on investigating the effects of salt stress on the chloroplasts proteome of salt-tolerant xylophytes.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of changes in the Kandelia candel chloroplast proteins reveals pathways associated with salt tolerance

Wang

Pan

et al. 2015

Plant Science

View full text Add to dashboard Cite

“…Subtractive hybridization (SH) is one such tool [ 19 ], which has been widely used in various organisms including plants [ 18 , 20 , 21 ]. Despite several transcriptomic studies carried out to identify genes responsible for salt tolerance in other mangroves such as Bruguiera and Aegiceras [ 22 - 24 ], the molecular mechanisms regulating salt secretion have not been established so far. The mangrove salt glands occur primarily on the leaf epidermis.…”

Section: Introductionmentioning

confidence: 99%

Identification of salt gland-associated genes and characterization of a dehydrin from the salt secretor mangrove Avicennia officinalis

et al. 2014

View full text Add to dashboard Cite

BackgroundSalt stress is a major challenge for growth and development of plants. The mangrove tree Avicennia officinalis has evolved salt tolerance mechanisms such as salt secretion through specialized glands on its leaves. Although a number of structural studies on salt glands have been done, the molecular mechanism of salt secretion is not clearly understood. Also, studies to identify salt gland-specific genes in mangroves have been scarce.ResultsBy subtractive hybridization (SH) of cDNA from salt gland-rich cell layers (tester) with mesophyll tissues as the driver, several Expressed Sequence Tags (ESTs) were identified. The major classes of ESTs identified include those known to be involved in regulating metabolic processes (37%), stress response (17%), transcription (17%), signal transduction (17%) and transport functions (12%). A visual interactive map generated based on predicted functional gene interactions of the identified ESTs suggested altered activities of hydrolase, transmembrane transport and kinases. Quantitative Real-Time PCR (qRT-PCR) was carried out to validate the expression specificity of the ESTs identified by SH. A Dehydrin gene was chosen for further experimental analysis, because it is significantly highly expressed in salt gland cells, and dehydrins are known to be involved in stress remediation in other plants. Full-length Avicennia officinalis Dehydrin1 (AoDHN1) cDNA was obtained by Rapid Amplification of cDNA Ends. Phylogenetic analysis and further characterization of this gene suggested that AoDHN1 belongs to group II Late Embryogenesis Abundant proteins. qRT-PCR analysis of Avicennia showed up-regulation of AoDHN1 in response to salt and drought treatments. Furthermore, some functional insights were obtained by growing E. coli cells expressing AoDHN1. Growth of E. coli cells expressing AoDHN1 was significantly higher than that of the control cells without AoDHN1 under salinity and drought stresses, suggesting that the mangrove dehydrin protein helps to mitigate the abiotic stresses.ConclusionsThirty-four ESTs were identified to be enriched in salt gland-rich tissues of A. officinalis leaves. qRT-PCR analysis showed that 10 of these were specifically enriched in the salt gland-rich tissues. Our data suggest that one of the selected genes, namely, AoDHN1 plays an important role to mitigate salt and drought stress responses.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0291-6) contains supplementary material, which is available to authorized users.

show abstract

Transcriptome Profiling of the Mangrove PlantBruguiera gymnorhizaand Identification of Salt Tolerance Genes byAgrobacteriumFunctional Screening

Cited by 26 publications

References 31 publications

A multilevel investigation to discover why Kandelia candel thrives in high salinity

A multilevel investigation to discover why Kandelia candel thrives in high salinity

Proteomic analysis of changes in the Kandelia candel chloroplast proteins reveals pathways associated with salt tolerance

Identification of salt gland-associated genes and characterization of a dehydrin from the salt secretor mangrove Avicennia officinalis

Contact Info

Product

Resources

About