A multilevel investigation to discover why <i>Kandelia candel</i> thrives in high salinity

Wang, Lingxia; Pan, Dezhuo; Lv, Xiaoyang; Cheng, Chi‐Lien; Li, Jian; Liang, Wei; Jian-hong, Xing; Chen, Wei

doi:10.1111/pce.12804

Cited by 36 publications

(49 citation statements)

References 53 publications

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“…Our results revealed that AMF inoculation enhanced ROS removal in P. tenuiflora seedlings. Moreover, most plants can accumulate compatible solutes, such as primary and secondary metabolites, to protect against cellular damage caused by ROS (Wang et al, ).…”

Section: Discussionmentioning

confidence: 99%

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

et al. 2019

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Alkali soil is a major abiotic constraint that limits plant distribution and yield in the northeast of China. Puccinellia tenuiflora is considered the most promising grass species for salt‐alkali grassland restoration. However, there is little information on the molecular mechanisms underlying how arbuscular mycorrhizal fungi (AMF) enhances P. tenuiflora stress responses in alkali‐degraded soil. In this study, AMF colonization, growth, photosynthetic pigments, and inorganic ion contents were measured. Isobaric tags for relative and absolute quantification‐based quantitative proteomic technology were employed to identify the differentially abundant proteins in P. tenuiflora seedlings with or without AMF under alkalinity stress. The results showed that AMF colonization increased seedling biomass, photosynthetic pigment contents, and K+/Na+ ratio under alkalinity stress. Moreover, a total of 598 proteins were significantly differentially regulated in P. tenuiflora seedlings after AMF inoculation under alkalinity stress compared with under alkalinity stress alone. The results showed that AMF inoculation significantly improved protein synthesis, reactive oxygen species scavenging, and nitrogen metabolism to promote the biosynthesis of osmotic substances in response to alkalinity stress. In addition, P. tenuiflora seedlings produced more energy to compensate for the energy loss caused by alkalinity stress after AMF inoculation. In conclusion, these findings provide new insights into the physiological mechanisms of the response to alkali‐degraded soil in P. tenuiflora seedlings with AMF and also clarify the role of AMF in the molecular regulation network of P. tenuiflora under alkalinity stress.

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Section: Discussionmentioning

confidence: 99%

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

et al. 2019

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“…Total RNA from the K. candel roots was also extracted using the CTAB-LiCl precipitation method (Jordon et al, 2015). Twelve DEGs were selected and analyzed by qRT-PCR using the housekeeping gene Actin as the internal reference gene (Wang et al, 2016). The primers which were designed according to the sequences obtained from transcriptome sequencing data were listed in Table 1 (see also Table S1).…”

Section: Quantitative Real Time-pcr (Qrt-pcr) Analysis Of Degsmentioning

confidence: 99%

“…GABA and Pro can be used as osmotic regulators to regulate the balance of plant internal environment (Akçay et al, 2012). Wang et al (2016) found that the contents of Glu and GABA in K. candel leaves increased significantly, but the contents of Pro did not change obviously under salt stress. In the present study, both P5CS and PDH2 genes were upregulated, which could explain why Pro was not accumulated with salt treatments in K. candel roots.…”

Section: Genes Related To Glu and Gaba Biosynthesismentioning

confidence: 99%

Transcriptomic insights into the molecular aspects of salt stress responses in Kandelia candel roots

2020

Turk J Bot

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The mangrove plant Kandelia candel is a type of woody halophyte that grows in tropical and subtropical ocean intertidal zones and exhibits a high salt tolerance. In this study, 61,970 unigenes were obtained from the roots of 60-day-old K. candel seedlings treated with 0 (control), 200, 400, and 600 mM NaCl for 3 days with an N50 of 1510 bp. Moreover, 454, 311, and 2663 genes were differentially expressed under 200, 400, and 600 mM NaCl treatments, respectively. These differentially expressed genes were primarily involved in plant hormone signal transduction, carbohydrate and energy metabolism, amino acid metabolism, stress response, and defense. The levels of 12 important differentially expressed genes were confirmed by qRT-PCR, showing that the changing trend was generally consistent with the results of the transcriptomic analysis. In addition, physiological parameters involved in energy metabolism, amino acid metabolism, and the reactive oxygen species scavenging process were significantly increased under salt stress treatment, and the trend was consistent with the results of transcription and qRT-PCR. This study indicated that K. candel roots could tolerate high salt stress by enhancing ethylene signal transduction, maintaining a stable energy supply, increasing antioxidant capacity. Specially, we found that accumulation of γ-aminobutyric acid and glutamate, but not proline, might play an important role in salt tolerance in the roots of K. candel.

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“…Hoagland nutrient solution was added to the sand culture. The lost water was supplemented every evening, and the nutrient solution was replaced every three days (Wang et al, 2016b). Until the plant grew four leaves (60 days), the seedlings were irrigated with Hoagland nutrient solution without NaCl (control group) and 600 mmol L -1 NaCl.…”

Section: Plant Materials and Salt Treatmentmentioning

confidence: 99%

Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress

Jian-hong¹,

Pan²,

Wang³

et al. 2019

Turk J Biol

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Kandelia candel is one of the mangrove species that are most resistant to environmental stress. As a typical nonsalt-secreting mangrove plant, K. candel is an ideal biological material to analyze the molecular mechanism of salt tolerance in woody plants. In this study, changes in protein abundance and expression profile in K. candel roots under high-salinity stress of 600 mmol L -1 NaCl were analyzed using isobaric tags for relative and absolute quantification (iTRAQ) assay. Moreover, the physiological parameters associated with metabolic pathways in which the differentially abundant proteins (DAPs) are involved were determined. A total of 5577 proteins were identified by iTRAQ analysis of the K. candel root proteins, of which 227 were DAPs with a fold change ratio >1.2 or a fold change ratio <0.83 and a P-value <0.05. A total of 227 DAPs consisting of 110 up-regulated and 117 down-regulated proteins were identified. Our Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the DAPs were primarily involved in biological processes including carbohydrate and energy metabolisms, stress response and defense, cell wall structure, and secondary metabolism. The results of the physiological parameters showed that their profile changes were consistent with those of the proteome analysis. The results of the proteome and physiological parameters showed that K. candel roots could resist highsalinity stress by maintaining a normal Embden-Meyerhof-Parnas and tricarboxylic acid (EMP-TCA) pathway, increasing the activities of various antioxidant enzymes and antioxidant contents, stabilizing the cell wall structure, and accumulating secondary metabolites such as triterpenoids.

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A multilevel investigation to discover why Kandelia candel thrives in high salinity

Cited by 36 publications

References 53 publications

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

Transcriptomic insights into the molecular aspects of salt stress responses in Kandelia candel roots

Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress

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