Transcriptome profiling of insulin sensitive tissues from GH deficient mice following GH treatment

Young, J. A.; Buchman, Mathew; Duran‐Ortiz, Silvana; Kruse, Colin; Bell, Stephen; Kopchick, John J.; Berryman, Darlene E.; List, Edward O.

doi:10.1007/s11102-020-01118-z

Cited by 5 publications

(2 citation statements)

References 51 publications

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“…RNASeq analysis of GH-Injected Mice—Male GH knockout mice generated as previously described [ 19 ] were injected at 4 months of age with recombinant human GH (5 μg/g body weight, Protein Laboratories Rehovot) or vehicle daily for five days and dissected four hours after the final injection. RNA was extracted from frozen liver tissue using a QIAGEN RNeasy mini kit according to manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

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Growth Hormone Alters Circulating Levels of Glycine and Hydroxyproline in Mice

et al. 2023

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Growth hormone (GH) has established effects on protein metabolism, such as increasing protein synthesis and decreasing amino acid degradation, but its effects on circulating amino acid levels are less studied. To investigate this relationship, metabolomic analyses were used to measure amino acid concentrations in plasma and feces of mice with alterations to the GH axis, namely bovine GH transgenic (bGH; increased GH action) and GH receptor knockout (GHRKO; GH resistant) mice. To determine the effects of acute GH treatment, GH-injected GH knockout (GHKO) mice were used to measure serum glycine. Furthermore, liver gene expression of glycine metabolism genes was assessed in bGH, GHRKO, and GH-injected GHKO mice. bGH mice had significantly decreased plasma glycine and increased hydroxyproline in both sexes, while GHRKO mice had increased plasma glycine in both sexes and decreased hydroxyproline in males. Glycine synthesis gene expression was decreased in bGH mice (Shmt1 in females and Shmt2 in males) and increased in GHRKO mice (Shmt2 in males). Acute GH treatment of GHKO mice caused decreased liver Shmt1 and Shmt2 expression and decreased serum glycine. In conclusion, GH alters circulating glycine and hydroxyproline levels in opposing directions, with the glycine changes at least partially driven by decreased glycine synthesis.

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Section: Methodsmentioning

confidence: 99%

“…RNA was extracted from frozen liver tissue using a QIAGEN RNeasy mini kit according to manufacturer’s instructions. RNA sequencing was performed as described previously [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

Growth Hormone Alters Circulating Levels of Glycine and Hydroxyproline in Mice

et al. 2023

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Growth hormone insensitivity and adipose tissue: tissue morphology and transcriptome analyses in pigs and humans

Young,

Hinrichs,

Bell

et al. 2023

Pituitary

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A non-invasive mouse model that recapitulates disuse-induced muscle atrophy in immobilized patients

Wu,

Lin,

Chu

et al. 2023

Sci Rep

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Disuse muscle atrophy occurs consequent to prolonged limb immobility or bed rest, which represents an unmet medical need. As existing animal models of limb immobilization often cause skin erosion, edema, and other untoward effects, we here report an alternative method via thermoplastic immobilization of hindlimbs in mice. While significant decreases in the weight and fiber size were noted after 7 days of immobilization, no apparent skin erosion or edema was found. To shed light onto the molecular mechanism underlying this muscle wasting, we performed the next-generation sequencing analysis of gastrocnemius muscles from immobilized versus non-mobilized legs. Among a total of 55,487 genes analyzed, 787 genes were differentially expressed (> fourfold; 454 and 333 genes up- and down-regulated, respectively), which included genes associated with muscle tissue development, muscle system process, protein digestion and absorption, and inflammation-related signaling. From a clinical perspective, this model may help understand the molecular/cellular mechanism that drives muscle disuse and identify therapeutic strategies for this debilitating disease.

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Transcriptome profiling of insulin sensitive tissues from GH deficient mice following GH treatment

Cited by 5 publications

References 51 publications

Growth Hormone Alters Circulating Levels of Glycine and Hydroxyproline in Mice

Growth Hormone Alters Circulating Levels of Glycine and Hydroxyproline in Mice

Growth hormone insensitivity and adipose tissue: tissue morphology and transcriptome analyses in pigs and humans

A non-invasive mouse model that recapitulates disuse-induced muscle atrophy in immobilized patients

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