Growth hormone insensitivity and adipose tissue: tissue morphology and transcriptome analyses in pigs and humans

Young, Jonathan A.; Hinrichs, Arne; Bell, Stephen; Geitgey, Delaney K.; Hume-Rivera, Diana; Bounds, Addison; Soneson, Maggie; Laron, Zvi; Yaron-Shaminsky, Danielle; Wolf, Eckhard; List, Edward O.; Kopchick, John J.; Berryman, Darlene E.

doi:10.1007/s11102-023-01355-y

Pituitary

2023

DOI: 10.1007/s11102-023-01355-y

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Growth hormone insensitivity and adipose tissue: tissue morphology and transcriptome analyses in pigs and humans

Jonathan A. Young,

Arne Hinrichs,

Stephen Bell

et al.

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2024

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Cited by 1 publication

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Decreased β-cell volume and insulin secretion but preserved glucose tolerance in a growth hormone insensitive pig model

Laane,

Renner,

Kemter

et al. 2024

Pituitary

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Purpose Growth hormone (GH) is a central regulator of β-cell proliferation, insulin secretion and sensitivity. Aim of this study was to investigate the effect of GH insensitivity on pancreatic β-cell histomorphology and consequences for metabolism in vivo. Methods Pancreata from pigs with growth hormone receptor deficiency (GHR-KO, n = 12) were analyzed by unbiased quantitative stereology in comparison to wild-type controls (WT, n = 12) at 3 and 7–8.5 months of age. In vivo secretion capacity for insulin and glucose tolerance were assessed by intravenous glucose tolerance tests (ivGTTs) in GHR-KO (n = 3) and WT (n = 3) pigs of the respective age groups. Results Unbiased quantitative stereological analyses revealed a significant reduction in total β-cell volume (83% and 73% reduction in young and adult GHR-KO vs. age-matched WT pigs; p < 0.0001) and volume density of β-cells in the pancreas of GHR-KO pigs (42% and 39% reduction in young and adult GHR-KO pigs; p = 0.0018). GHR-KO pigs displayed a significant, age-dependent increase in the proportion of isolated β-cells in the pancreas (28% in young and 97% in adult GHR-KO vs. age-matched WT pigs; p = 0.0009). Despite reduced insulin secretion in ivGTTs, GHR-KO pigs maintained normal glucose tolerance. Conclusion GH insensitivity in GHR-KO pigs leads to decreased β-cell volume and volume proportion of β-cells in the pancreas, causing a reduced insulin secretion capacity. The increased proportion of isolated β-cells in the pancreas of GHR-KO pigs highlights the dependency on GH stimulation for proper β-cell maturation. Preserved glucose tolerance accomplished with decreased insulin secretion indicates enhanced sensitivity for insulin in GH insensitivity.

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Decreased β-cell volume and insulin secretion but preserved glucose tolerance in a growth hormone insensitive pig model

Laane,

Renner,

Kemter

et al. 2024

Pituitary

View full text Add to dashboard Cite

show abstract

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Growth hormone insensitivity and adipose tissue: tissue morphology and transcriptome analyses in pigs and humans

Cited by 1 publication

References 53 publications

Decreased β-cell volume and insulin secretion but preserved glucose tolerance in a growth hormone insensitive pig model

Decreased β-cell volume and insulin secretion but preserved glucose tolerance in a growth hormone insensitive pig model

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