Transcriptome profiling based on larvae at different time points after hatching provides a core set of gene resource for understanding the immune response mechanisms of the egg-protecting behavior against Vibrio anguillarum infection in Amphioctopus fangsiao

Bao, Xiaokai; Yuan, Tingzhu; Li, Yan; Chen, Xipan; Liu, Xiumei; Xu, Xiaohui; Sun, Guohua; Li, Bin; Yang, Jianmin; Feng, Yanwei; Li, Zan

doi:10.1016/j.fsi.2022.04.030

Cited by 10 publications

(2 citation statements)

References 95 publications

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“…In recent years, there has been considerable progress in high-throughput transcriptome sequencing technology, and biological analysis through high-throughput transcriptome sequencing has become more accurate ( Morozova et al, 2009 ; Qian et al, 2014 ; Bao et al, 2022a ; Bao et al, 2022b ). To illustrate, through such technology, the expression of heat stress genes in Pinctada fucata have been found to be significantly reduced after being stimulated by multiple high temperatures, indicating that P. fucata is able to gradually adapt to the impact of high temperatures on their life activities ( Li et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Exploration of anti-stress mechanisms in high temperature exposed juvenile golden cuttlefish (Sepia esculenta) based on transcriptome profiling

Wang

Bao

et al. 2023

Front. Physiol.

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Sepia esculenta is a cephalopod widely distributed in the Western Pacific Ocean, and there has been growing research interest due to its high economic and nutritional value. The limited anti-stress capacity of larvae renders challenges for their adaptation to high ambient temperatures. Exposure to high temperatures produces intense stress responses, thereby affecting survival, metabolism, immunity, and other life activities. Notably, the molecular mechanisms by which larval cuttlefish cope with high temperatures are not well understood. As such, in the present study, transcriptome sequencing of S. esculenta larvae was performed and 1,927 differentially expressed genes (DEGs) were identified. DEGs were subjected to functional enrichment analyses using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The top 20 terms of biological processes in GO and 20 high-temperature stress-related pathways in KEGG functional enrichment analysis were identified. A protein-protein interaction network was constructed to investigate the interaction between temperature stress-related genes. A total of 30 key genes with a high degree of participation in KEGG signaling pathways or protein-protein interactions were identified and subsequently validated using quantitative RT-PCR. Through a comprehensive analysis of the protein-protein interaction network and KEGG signaling pathway, the functions of three hub genes (HSP90AA1, PSMD6, and PSMA5), which belong to the heat shock protein family and proteasome, were explored. The present results can facilitate further understanding of the mechanism of high temperature resistance in invertebrates and provide a reference for the S. esculenta industry in the context of global warming.

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Section: Introductionmentioning

confidence: 99%

Exploration of anti-stress mechanisms in high temperature exposed juvenile golden cuttlefish (Sepia esculenta) based on transcriptome profiling

Wang

Bao

et al. 2023

Front. Physiol.

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“…Pathogens that cause high rates of disease in marine aquaculture in A. fangsiao include Vibrio anguillarum , Vibrio Parahemolyticus , and Edwardsiella tarda . Infected A. fangsiao can show varying degrees of tissue damage, leading to increased culture mortality [ 13 , 14 , 15 ]. Lipopolysaccharides (LPS) and polyinosinic-polycytidylic acid (Poly I:C), both recognized by pattern recognition receptors (PRRs), are typically employed to mimic bacterial and viral invasions, respectively [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Weighted Gene Co-Expression Network Analysis Based on Stimulation by Lipopolysaccharides and Polyinosinic:polycytidylic Acid Provides a Core Set of Genes for Understanding Hemolymph Immune Response Mechanisms of Amphioctopus fangsiao

Wang,

Chen,

et al. 2023

Animals

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The primary influencer of aquaculture quality in Amphioctopus fangsiao is pathogen infection. Both lipopolysaccharides (LPS) and polyinosinic:polycytidylic acid (Poly I:C) are recognized by the pattern recognition receptor (PRR) within immune cells, a system that frequently serves to emulate pathogen invasion. Hemolymph, which functions as a transport mechanism for immune cells, offers vital transcriptome information when A. fangsiao is exposed to pathogens, thereby contributing to our comprehension of the species’ immune biological mechanisms. In this study, we conducted analyses of transcript profiles under the influence of LPS and Poly I:C within a 24 h period. Concurrently, we developed a Weighted Gene Co-expression Network Analysis (WGCNA) to identify key modules and genes. Further, we carried out Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses to investigate the primary modular functions. Co-expression network analyses unveiled a series of immune response processes following pathogen stress, identifying several key modules and hub genes, including PKMYT1 and NAMPT. The invaluable genetic resources provided by our results aid our understanding of the immune response in A. fangsiao hemolymph and will further our exploration of the molecular mechanisms of pathogen infection in mollusks.

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The Application of Molecular Markers in Fish Breeding and Aquaculture

Rodrigues

Biun

Yong

et al. 2023

Marine Biotechnology: Applications in Food, Drugs and Energy

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Transcriptome profiling based on larvae at different time points after hatching provides a core set of gene resource for understanding the immune response mechanisms of the egg-protecting behavior against Vibrio anguillarum infection in Amphioctopus fangsiao

Cited by 10 publications

References 95 publications

Exploration of anti-stress mechanisms in high temperature exposed juvenile golden cuttlefish (Sepia esculenta) based on transcriptome profiling

Exploration of anti-stress mechanisms in high temperature exposed juvenile golden cuttlefish (Sepia esculenta) based on transcriptome profiling

Weighted Gene Co-Expression Network Analysis Based on Stimulation by Lipopolysaccharides and Polyinosinic:polycytidylic Acid Provides a Core Set of Genes for Understanding Hemolymph Immune Response Mechanisms of Amphioctopus fangsiao

The Application of Molecular Markers in Fish Breeding and Aquaculture

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