Transcriptome profiling and dimorphic expression of sex-related genes in fifth-instar nymphs of Sogatella furcifera, an important rice pest

Jia, Lin; He, Jingyi; Liang, Anwen; Wang, Fanghai

doi:10.1016/j.ygeno.2019.06.024

Cited by 7 publications

(7 citation statements)

References 34 publications

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“…Interestingly, 6 genes in the top 10 upregulated female-specific genes [27] were even significantly upregulated in Sf-V and Sf-NV (Table 5). The RT-qPCR detection of these female-specific genes confirmed that SRBSDV exposure activated the expression of these genes, which were consistent with transcriptome results (Figure 5).…”

Section: Discussionmentioning

confidence: 99%

“…Among the top 10 female-specific genes identified in S. furcifera [27], 6 genes were found in the DEGs of S. furcifera females acquired SRBSDV or not in comparison with the control. Expressions of the six DEGs were significantly upregulated in Sf-NV females compared with those in CK females, while only three DEGs were more expressed in Sf-V females than in CK females (Table 5).…”

Section: Expression Of Female-specific Genes In S Furciferamentioning

confidence: 99%

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Comparison of Transcriptome Responses between Sogatella furcifera Females That Acquired Southern Rice Black-Streaked Dwarf Virus and Not

Liu

Hou

2022

Insects

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The southern rice black-streaked dwarf virus (SRBSDV) is transmitted horizontally by Sogatella furcifera in a persistent, propagative manner. Exposure of S. furcifera females to SRBSDV-infected rice plants may trigger transcriptomic changes in the insects, the transcriptomes of females that acquired SRBSDV and those that failed to, as well as females fed on healthy rice plants as control, were sequenced and compared. Nine transcriptomic libraries were constructed, from which a total of 53,084 genes were assembled. Among the genes, 1043 and 2932 were differentially expressed genes (DEGs) in S. furcifera females that acquired SRBSDV and that failed to, in comparison with the control, respectively. Functional enrichment analysis showed that DEGs identified in S. furcifera females exposed to SRBSDV are primarily involved in diverse signaling pathways related to primary metabolism and innate immunity. The DEGs in the S. furcifera females that failed to acquire the virus significantly outnumbered that in the insects that acquired the virus, and the virus exposure activated the humoral and cellular immune responses of the vectors, especially the apoptosis. The key gene in apoptosis encoding caspase 1 was upregulated by SRBSDV exposure, especially in S. furcifera females that failed to acquire the virus. Analysis of caspase 1 activity validated that SRBSDV exposure induced caspase 1 accumulation. Surprisingly, the expression of six female-specific genes was also upregulated by SRBSDV exposure, which was confirmed by RT-qPCR analysis. This study provides evidence to explain the differential virus acquisition at the transcriptome level.

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Section: Discussionmentioning

confidence: 99%

Section: Expression Of Female-specific Genes In S Furciferamentioning

confidence: 99%

Comparison of Transcriptome Responses between Sogatella furcifera Females That Acquired Southern Rice Black-Streaked Dwarf Virus and Not

Liu

Hou

2022

Insects

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“…It has been used for the screening of genes associated with various traits in sugarcane, such as sucrose accumulation [ 39 , 40 ], smut resistance [ 41 , 42 ] and the response to low nitrogen [ 43 ]. Transcriptome analysis was also used for the identification of sex-determining genes in many insects, such as Plutella xylostella [ 44 ], Phlebotominae sand flies [ 45 ], whitefly [ 46 ], Sogatella furcifera [ 47 ] and Zeugodacus tau (Walker) [ 48 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling Reveals Genes Related to Sex Determination and Differentiation in Sugarcane Borer (Chilo sacchariphagus Bojer)

Wei

et al. 2022

Insects

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Chilo sacchariphagus Bojer is an important sugarcane pest globally. Along with genetic modification strategies, the sterile insect technique (SIT) has gained more attention as an environment-friendly method for pest control. The identification of key genes associated with sex determination and differentiation will provide important basic information for this control strategy. As such, the transcriptome sequencing of female and male adults was conducted in order to understand the sex-biased gene expression and molecular basis of sex determination and differentiation in this species. A total of 60,429 unigenes were obtained; among them, 34,847 genes were annotated. Furthermore, 11,121 deferentially expressed genes (DEGs) were identified, of which 8986 were male-biased and 2135 were female-biased genes. The male-biased genes were enriched for carbon metabolism, peptidase activity and transmembrane transport, while the female-biased genes were enriched for the cell cycle, DNA replication, and the MAPK signaling pathway. In addition, 102 genes related to sex-determination and differentiation were identified, including the protein toll, ejaculatory bulb-specific protein, fruitless, transformer-2, sex-lethal, beta-Catenin, sox, gata4, beta-tubulin, cytosol aminopeptidase, seminal fluid, and wnt4. Furthermore, transcription factors such as myb, bhlh and homeobox were also found to be potentially related to sex determination and differentiation in this species. Our data provide new insights into the genetic elements associated with sex determination and differentiation in Chilo sacchariphagus, and identified potential candidate genes to develop pest-control strategies.

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“…Thus, we first examined the feeding behavior of the rice pest S. furcifera after M. anisopliae infection. Transcriptomic profiling is a useful tool to investigate and screen for potential targets under different conditions [ 39 , 40 ]. To understand the potential mechanisms, and to seek the potential olfactory-related targets, we further studied the response of S. furcifera to M. anisopliae infection under different periods by transcriptomic analysis.…”

Section: Introductionmentioning

confidence: 99%

The Entomopathogenic Fungus Metarhizium anisopliae Affects Feeding Preference of Sogatella furcifera and Its Potential Targets’ Identification

Wang

Han

Xia

et al. 2022

JoF

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The rice planthopper Sogatella furcifera is a unique vector of the southern rice black-streaked dwarf virus (SRBSDV). The feeding behavior of S. furcifera should directly affect the diffusion of this virus. In this study, we noted that the infection of Metarhizium anisopliae CQMa421 on S. furcifera disturbed the feeding behavior of this pest to SRBSDV-infected rice, from preference to non-preference. Then, we further investigated the potential targets of M. anisopliae CQMa421 on the feeding behavior of S. furcifera after 0 h, 24 h and 48 h of infection by transcriptomic analysis via Illumina deep sequencing. A total of 93.27 GB of data was collected after sequencing, from which 91,125 unigenes were annotated, including 75 newly annotated genes. There were 1380 vs. 2187 and 137 vs. 106 upregulated and downregulated differentially expressed genes (DEGs) detected at 24 h and 48 h, respectively. The biological functions and associated metabolic processes of these genes were determined with the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The results suggested that major of DEGs are involved in energy metabolism, biosynthesis, immune response, the FoxO signaling pathway, the MAPK signaling pathway and apoptosis in response to the fungal infection. Noteworthily, several olfactory-related genes, including odorant receptors and odorant binding proteins, were screened from these differentially expressed genes, which played critical roles in regulating the olfactory behavior of insects. Taken together, these results provide new insights for understanding the molecular mechanisms underlying fungus and host insect interaction, especially for olfactory behavior regulated by fungus.

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Transcriptome profiling and dimorphic expression of sex-related genes in fifth-instar nymphs of Sogatella furcifera, an important rice pest

Cited by 7 publications

References 34 publications

Comparison of Transcriptome Responses between Sogatella furcifera Females That Acquired Southern Rice Black-Streaked Dwarf Virus and Not

Comparison of Transcriptome Responses between Sogatella furcifera Females That Acquired Southern Rice Black-Streaked Dwarf Virus and Not

Transcriptome Profiling Reveals Genes Related to Sex Determination and Differentiation in Sugarcane Borer (Chilo sacchariphagus Bojer)

The Entomopathogenic Fungus Metarhizium anisopliae Affects Feeding Preference of Sogatella furcifera and Its Potential Targets’ Identification

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