Transcriptome Profile Alteration with Cadmium Selenide/Zinc Sulfide Quantum Dots in Saccharomyces cerevisiae

Horstmann, Cullen; Kim, Daniel S.; Campbell, Chelsea; Kim, Dong Kyu

doi:10.3390/biom9110653

Cited by 14 publications

(19 citation statements)

References 58 publications

(87 reference statements)

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“…The only common enzyme to the 9 and 24 h exposure times was Ald4, the level of which initially increased at 9 h and decreased at 24 h. As reported earlier, ENMs treatment inhibits the glycolytic pathway and stimulate fermentation [ 56 ]. Horstmann et al, (2019) suggested that sugar transport genes and sugar-utilising enzyme genes are simultaneously affected by the presence of Cd-QDs [ 57 ]. The two isoforms of GAPDH (Tdh1, Tdh2) were found to be upregulated for both treatment times.…”

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis Identifies Markers of Exposure to Cadmium Sulphide Quantum Dots (CdS QDs)

et al. 2020

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The use of cadmium sulphide quantum dot (CdS QD)-enabled products has become increasingly widespread. The prospect of their release in the environment is raising concerns. Here we have used the yeast model Saccharomyces cerevisiae to determine the potential impact of CdS QD nanoparticles on living organisms. Proteomic analyses and cell viability assays performed after 9 h exposure revealed expression of proteins involved in oxidative stress and reduced lethality, respectively, whereas oxidative stress declined, and lethality increased after 24 h incubation in the presence of CdS QDs. Quantitative proteomics using the iTRAQ approach (isobaric tags for relative and absolute quantitation) revealed that key proteins involved in essential biological pathways were differentially regulated over the time course of the experiment. At 9 h, most of the glycolytic functions increased, and the abundance of the number of heat shock proteins increased. This contrasts with the situation at 24 h where glycolytic functions, some heat shock proteins as well as oxidative phosphorylation and ATP synthesis were down-regulated. It can be concluded from our data that cell exposure to CdS QDs provokes a metabolic shift from respiration to fermentation, comparable to the situation reported in some cancer cell lines.

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Section: Resultsmentioning

confidence: 99%

Proteomic Analysis Identifies Markers of Exposure to Cadmium Sulphide Quantum Dots (CdS QDs)

et al. 2020

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“…Through further studies, no noteworthy changes in superoxide were noticed, with or without CdSe/ZnS–QDs treatment. Overall, results through transcriptome analysis indicated the exposure of CdSe/ZnS–QDs within yeast remarkably affected genes associated in multiple cellular processes [ 143 ].…”

Section: Selenium In Physics Surfaces and Nanoscience With Regarmentioning

confidence: 99%

Discussions of Fluorescence in Selenium Chemistry: Recently Reported Probes, Particles, and a Clearer Biological Knowledge

et al. 2021

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In this review from literature appearing over about the past 5 years, we focus on selected selenide reports and related chemistry; we aimed for a digestible, relevant, review intended to be usefully interconnected within the realm of fluorescence and selenium chemistry. Tellurium is mentioned where relevant. Topics include selenium in physics and surfaces, nanoscience, sensing and fluorescence, quantum dots and nanoparticles, Au and oxide nanoparticles quantum dot based, coatings and catalyst poisons, thin film, and aspects of solar energy conversion. Chemosensing is covered, whether small molecule or nanoparticle based, relating to metal ion analytes, H2S, as well as analyte sulfane (biothiols—including glutathione). We cover recent reports of probing and fluorescence when they deal with redox biology aspects. Selenium in therapeutics, medicinal chemistry and skeleton cores is covered. Selenium serves as a constituent for some small molecule sensors and probes. Typically, the selenium is part of the reactive, or active site of the probe; in other cases, it is featured as the analyte, either as a reduced or oxidized form of selenium. Free radicals and ROS are also mentioned; aggregation strategies are treated in some places. Also, the relationship between reduced selenium and oxidized selenium is developed.

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“…An assay to measure the reactive oxygen species (ROS) was performed over a three-day period as described before [14][15][16]. Initially, cells were cultured and counted.…”

Section: Reactive Oxygen Species Assaymentioning

confidence: 99%

“…The wells were mixed thoroughly by pipette and centrifuge, and the plate was put into QuantStudio 6 Pro instrument (Thermo Fisher Scientific, Waltham, MA, USA) for PCR amplification. Changes were detected in comparison to the housekeeping gene (CCT2) using the Pfaffl method [14,15,17]; the gene expression ratio of each target gene was calculated based on E (RT-qPCR efficiencies) and Cq value deviation from the control (CCT2 expression levels).…”

Section: Rt-qpcrmentioning

confidence: 99%

An Assessment of InP/ZnS as Potential Anti-Cancer Therapy: Quantum Dot Treatment Increases Apoptosis in HeLa Cells

Davenport

Horstmann

Patel

et al. 2021

JNT

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InP/ZnS quantum dots (QDs) are an emerging option in QD technologies for uses of fluorescent imaging as well as targeted drug and anticancer therapies based on their customizable properties. In this study we explored effects of InP/ZnS when treated with HeLa cervical cancer cells. We employed XTT viability assays, reactive oxygen species (ROS) analysis, and apoptosis analysis to better understand cytotoxicity extents at different concentrations of InP/ZnS. In addition, we compared the transcriptome profile from the QD-treated HeLa cells with that of untreated HeLa cells to identify changes to the transcriptome in response to the QD. RT-qPCR assay was performed to confirm the findings of transcriptome analysis, and the QD mode of action was illustrated. Our study determined both IC50 concentration of 69 µg/mL and MIC concentration of 167 µg/mL of InP/ZnS. It was observed via XTT assay that cell viability was decreased significantly at the MIC. Production of superoxide, measured by ROS assay with flow cytometry, was decreased, whereas levels of nitrogen radicals increased. Using analysis of apoptosis, we found that induced cell death in the QD-treated samples was shown to be significantly increased when compared to untreated cells. We conclude InP/ZnS QD to decrease cell viability by inducing stress via ROS levels, apoptosis induction, and alteration of transcriptome.

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Transcriptome Profile Alteration with Cadmium Selenide/Zinc Sulfide Quantum Dots in Saccharomyces cerevisiae

Cited by 14 publications

References 58 publications

Proteomic Analysis Identifies Markers of Exposure to Cadmium Sulphide Quantum Dots (CdS QDs)

Proteomic Analysis Identifies Markers of Exposure to Cadmium Sulphide Quantum Dots (CdS QDs)

Discussions of Fluorescence in Selenium Chemistry: Recently Reported Probes, Particles, and a Clearer Biological Knowledge

An Assessment of InP/ZnS as Potential Anti-Cancer Therapy: Quantum Dot Treatment Increases Apoptosis in HeLa Cells

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