Transcriptome comparison of winter and spring wheat responding to low temperature

Abstract

Freezing tolerance in plants is a complex trait that occurs in many plant species during growth at low, nonfreezing temperatures, a process known as cold acclimation. This process is regulated by a multigenic system expressing broad variation in the degree of freezing tolerance among wheat cultivars. Microarray analysis is a powerful and rapid approach to gene discovery. In species such as wheat, for which large scale mutant screening and transgenic studies are not currently practical, genotype comparison by this methodology represents an essential approach to identifying key genes in the acquisition of freezing tolerance. A microarray was constructed with PCR amplified cDNA inserts from 1184 wheat expressed sequence tags (ESTs) that represent 947 genes. Gene expression during cold acclimation was compared in 2 cultivars with marked differences in freezing tolerance. Transcript levels of more than 300 genes were altered by cold. Among these, 65 genes were regulated differently between the 2 cultivars for at least 1 time point. These include genes that encode potential regulatory proteins and proteins that act in plant metabolism, including protein kinases, putative transcription factors, Ca2+ binding proteins, a Golgi localized protein, an inorganic pyrophosphatase, a cell wall associated hydrolase, and proteins involved in photosynthesis.Key words: wheat microarray, expression profile, plant transcription, cold-regulated genes, freezing tolerance, cold acclimation, winter hardiness, stress genes, gene regulation, wheat transcriptome.

Biological Activity of Essential Oils from Leaves and Fruits of Pepper Tree (Schinus molle L.) to Control Rice Weevil (Sitophilus oryzae L.)

“…A successful example is the comparison of the transcriptome of winter and spring wheat, cultivars with different tolerance to cold, exposed to low temperature. This study reports the correlation of gene expression kinetics with tolerance to low temperature, a subject usually overlooked that emphasizes the importance of sampling in functional genomics studies (Gulick et al, 2005;Monroy et al, 2007).Gene expression profiling has allowed the identification of hundreds of genes induced when plants are exposed to stress ( Kreps ( Kreps et al, 2002;Oono et al, 2006;Jianping and Suleiman, 2007;Mantri et al, 2007). The availability of…”

Effects of climate change on killing frost in the Canadian prairies

“…For LSKF, many studies found that the LT50 in the early growth stage of acclimated wheats including Canadian spring cultivars is −8°C (Macdowall 1974, Charron et al 2002, Gulick et al 2005, Ganeshan et al 2008. Slight vegetative damage may occur at these temperatures, but wheat plants can easily recover from this since the growing point is at or below the soil surface and is well buffered against fluctuating air temperatures.…”

Respuesta de los frutos cítricos a las bajas temperaturas: estudio mediante micromatrices

“…La aclimatación al frío está correlacionada con la activación de numerosos genes de repuesta a bajas temperaturas (Fowler y Thomashow, 2002). Estudios comparativos han podido relacionar diferencias en los niveles de expresión de mRNAs de genes inducidos por las bajas temperaturas con las diferencias en tolerancia al frío de algunos cultivares (Gulick et al, 2005;Monroy et al, 2007;Amtmann, 2009;Zhao et al, 2009).…”

“…La disponibilidad de numerosas colecciones de cDNA ha permitido la construcción de micromatrices para un gran número de especies vegetales, muchas de ellas con un alto interés agronómico, como arroz (Rabbani et al, 2003;Lian et al, 2006), maíz , soja (Vodkin et al, 2004;Ainsworth et al, 2006), patata (Stupar et al, 2007), tomate (Alba et al, 2005), fresa (Aharoni et al, 2004), trigo (Gulick et al, 2005), garbanzo (Mantri et al, 2007), girasol (Fernández et al, 2008), melocotón (Ogundiwin et al, 2008), y cítricos (Forment et al, 2005, Martinez-Godoy et al, 2008.…”

“…La metodología de las micromatrices ha permitido el análisis de genes de respuesta al frío en diversas especies vegetales, para un conocimiento básico derivado del modelo Arabidopsis (Seki et al, 2001(Seki et al, , 2002Chen et al, 2002;Kreps et al, 2002;Maruyama et al, 2004;Vergonelle et al, 2005;Oono et al, 2006;Benedict et al, 2006;Kilian et al, 2007;Swindell et al, 2007), y para la posible identificación de genes responsables de DF en cultivos de interés agronómico como arroz (Rabbani et al, 2003;Ito et al, 2006), pomelo (Maul et al, 2008), girasol (Fernandez et al, 2008), melocotón (Ogundiwin et al, 2008), trigo (Gulick et al, 2005 …”