Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melon

Duan, Xiaoyu; Jiang, Cai; Zhao, Yang; Gao, Ge; Li, Meng; Qi, Hongyan

doi:10.1016/j.hpj.2022.07.005

Cited by 27 publications

(14 citation statements)

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“…According to target metabolome analysis in a previous study, β-carotene is a major carotenoid metabolite in oriental melon, making the flesh of the ‘HDB’ fruit appear orange [ 33 ]. The results measured by HPLC showed that the β-carotene content of fruit was less than 10 μg·g −1 FW at the young fruit stage (from 10 to 20 days after anthesis, DAA), and the β-carotene content increased sharply at the mature stage from 21 μg·g −1 FW to 110 μg·g −1 FW (from 25 to 35 DAA) ( Figure 1 a).…”

Section: Resultsmentioning

confidence: 99%

“…The expression profiles of carotenoid metabolism-related genes were analyzed using the RT-qPCR in the previous study [ 33 ]. The results showed that these genes were initially upregulated and subsequently downregulated, peaking at 25 DAA, which implies that carotenoid accumulation was attributed to the expression of carotenoid biosynthesis-related genes.…”

Section: Resultsmentioning

confidence: 99%

“…The flesh color of oriental melon is a critical trait determined by carotenoid content and composition. β-carotene is the dominant carotenoid component, accounting for more than 50% of the total carotenoid in orange-flesh oriental melon reported by a previous study [ 33 ]. In the present study, CmNAC34 was identified by yeast one-hybrid screening with CmLCYB promoter, and its expression profile was consistent with that of CmLCYB during fruit ripening, and its transcript level was significantly higher in orange-fleshed oriental melon.…”

Section: Introductionmentioning

confidence: 99%

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Transcription Factor CmNAC34 Regulated CmLCYB-Mediated β-Carotene Accumulation during Oriental Melon Fruit Ripening

Zhao¹,

Duan²,

Wang³

et al. 2022

IJMS

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Ripened oriental melon (Cucumis melo) with orange-colored flesh is rich in β-carotene. Lycopene β-cyclase (LCYB) is the synthetic enzyme that directly controls the massive accumulation of β-carotene. However, the regulatory mechanism underlying the CmLCYB-mediated β-carotene accumulation in oriental melon is fairly unknown. Here, we screened and identified a transcription factor, CmNAC34, by combining bioinformatics analysis and yeast one-hybrid screen with CmLCYB promoter. CmNAC34 was located in the nucleus and acted as a transcriptional activator. The expression profile of CmNAC34 was consistent with that of CmLCYB during the fruit ripening. Additionally, the transient overexpression of CmNAC34 in oriental melon fruit promoted the expression of CmLCYB and enhanced β-carotene concentration, while transient silence of CmNAC34 in fruit was an opposite trend, which indicated CmNAC34 could modulate CmLCYB-mediated β-carotene biosynthesis in oriental melon. Finally, the yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), β-glucuronidase (GUS) analysis assay, and luciferase reporter (LUC) assay indicated that CmNAC34 could bind to the promoter of CmLCYB and positively regulated the CmLCYB transcription level. These findings suggested that CmNAC34 acted as an activator to regulate β-carotene accumulation by directly binding the promoter of CmLCYB, which provides new insight into the regulatory mechanism of carotenoid metabolism during the development and ripening of oriental melon.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcription Factor CmNAC34 Regulated CmLCYB-Mediated β-Carotene Accumulation during Oriental Melon Fruit Ripening

Zhao¹,

Duan²,

Wang³

et al. 2022

IJMS

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“…In citrus plants, CsERF061 , induced by ethylene, regulates carotenoid accumulation by directly activating the expression of LCYB2 and other key carotenoid pathway genes 30 . In oriental melon, CmWRKY49 and CmNAC34 participate in carotenoid biosynthesis by activating the expression of CmPSY1 and CmLCYB 31 .…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals association of carotenoid metabolism pathway with fruit color in melon

Diao

Tian

Cao

et al. 2023

Sci Rep

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Flesh color is an important quality of melon (Cucumis melo L.) and is determined mainly by carotenoid content, awarding them with colors, aromas, and nutrients. enhancing the nutritional and health benefits of fruits and vegetables for humans. In this study, we performed transcriptomic analysis of two melon inbred line “B-14” (orange-flesh) and “B-6” (white-flesh) at three developmental stages. We observed that the β-carotene content of inbred line “B-6” (14.232 μg/g) was significantly lower than that of inbred line “B-14” (0.534 μg/g). RNA-sequencing and quantitative reverse transcription PCR analyses were performed to identify differentially expressed genes (DEGs) between the two inbred lines at different stages; the DEGs were analyzed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes databases (KEGG). We identified 33 structural DEGs in different developmental periods of the two lines that were related to carotenoid metabolism. Among them, PSY, Z-ISO, ZDS, CRTISO, CCD4, VDE1, and NCED2 were highly correlated with carotenoid content. Thus, this study provides a basis for molecular mechanism of carotenoid biosynthesis and flesh color in melon fruit.

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“…These carotenes are further converted to xanthophylls through a series of hydroxylation reactions. In addition to the CBP genes, several transcriptional regulators, such as MYB, WRKY, MADS, bHLH, B-box, ERF, and NAC, that control the accumulation of carotenoids by directly interacting with the CBP genes to repress or activate their expression have been identified ( Welsch et al., 2007 ; Ma et al., 2014 ; Larkin et al., 2016 ; Sagawa et al., 2016 ; Fu et al., 2017 ; Lu et al., 2018 ; Ampomah-Dwamena et al., 2019 ; Meng et al., 2019 ; Xiong et al., 2019 ; Zhou et al., 2019 ; Duan et al., 2022 ). For example, WHITE PETAL1 (WP1), a R2R3-MYB protein, plays a crucial role in regulating floral carotenoid pigmentation in Medicago by directly regulating the expression of MtLYCe and MtLYCb ( Meng et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic mapping of a single nuclear locus determines the white flesh color in watermelon (Citrullus lanatus L.)

Zhou

Zhang

et al. 2023

Front. Plant Sci.

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IntroductionFlesh color is an important trait in watermelon (Citrullus lanatus L.). Several flesh color genes have been identified in watermelon; however, the inheritance of and the molecular basis underlying the white flesh trait remain largely unknown.MethodsIn this study, segregation populations were constructed by crossing the canary yellow flesh line HSH-F with the white flesh line Sanbai to fine-map the white flesh gene in watermelon.ResultsGenetic analysis indicated that the white flesh trait is controlled by a single recessive locus, termed Clwf2. Map-based cloning delimited the Clwf2 locus to a 132.3-kb region on chromosome 6. The candidate region contains 13 putative genes, and four of them—Cla97C06G121860, Cla97C06G121880, Cla97C06G121890, and Cla97C06G121900—were significantly downregulated in the white flesh compared to the canary yellow flesh watermelon fruits. The Cla97C06G121890 gene, which encodes a tetratricopeptide repeat protein, showed almost no expression in the white flesh fruit before maturity, whereas it had a very high expression in the canary yellow flesh fruit at 18 days after pollination. Transmission electron microscopy revealed rounded and regularly shaped chromoplasts in both the canary yellow and white flesh fruits. Further quantitative real-time PCR analysis showed that the expression levels of several key plastid division genes and almost the entire carotenoid biosynthesis pathway genes were downregulated in the white flesh compared to the canary yellow flesh fruits.DiscussionThis study suggests that the proliferation inhibition of chromoplasts and downregulation of the CBP genes block the accumulation of carotenoids in watermelon and lead to white flesh. These findings advance and extend the understanding of the molecular mechanisms underlying white flesh trait formation and carotenoid biosynthesis in watermelon.

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Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melon

Cited by 27 publications

References 55 publications

Transcription Factor CmNAC34 Regulated CmLCYB-Mediated β-Carotene Accumulation during Oriental Melon Fruit Ripening

Transcription Factor CmNAC34 Regulated CmLCYB-Mediated β-Carotene Accumulation during Oriental Melon Fruit Ripening

Transcriptome analysis reveals association of carotenoid metabolism pathway with fruit color in melon

Genetic mapping of a single nuclear locus determines the white flesh color in watermelon (Citrullus lanatus L.)

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