Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation among Olea europaea Fruit Tissues in Late Development

Briegas, B.; Corbacho, Jorge; Parra-Lobato, M.C.; Paredes, Miguel A.; Labrador, Juana; Gallardo, Mercedes; Gómez-Jiménez, M.C.

doi:10.3390/ijms21144819

Cited by 14 publications

(15 citation statements)

References 74 publications

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“…Moreover, this study found that the expressions of ethylene and ABA synthesis-related genes were significantly increased. Ethylene and ABA play a major role in regulating the ripening and softening of climacteric fruit and, accordingly, the expression of some ripening-related cell wall-associated genes and activities, including those of PGs, PMEs and CX [ 18 , 19 , 20 ]. ABA and ethylene are well known for their pivotal roles in stress response.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Analyses Provide Insights into the Watercore Disorder on “Akibae” Pear Fruit

Liu¹,

Fan²,

Liu³

et al. 2021

IJMS

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Watercore is a physiological disorder that commonly occurs in sand pear cultivars. The typical symptom of watercore tissue is transparency, and it is often accompanied by browning, breakdown and a bitter taste during fruit ripening. To better understand the molecular mechanisms of watercore affecting fruit quality, this study performed transcriptome and metabolome analyses on watercore pulp from “Akibae” fruit 125 days after flowering. The present study found that the “Akibae” pear watercore pulp contained higher sorbitol and sucrose than healthy fruit. Moreover, the structure of the cell wall was destroyed, and the content of pectin, cellulose and hemicellulose was significantly decreased. In addition, the content of ethanol and acetaldehyde was significantly increased, and the content of polyphenol was significantly decreased. Watercore induced up-regulated expression levels of sorbitol synthesis-related (sorbitol-6-phosphate dehydrogenase, S6PDH) and sucrose synthesis-related genes (sucrose synthesis, SS), whereas it inhibited the expression of sorbitol decomposition-related genes (sorbitol dehydrogenase, SDH) and sorbitol transport genes (sorbitol transporter, SOT). Watercore also strongly induced increased expression levels of cell wall-degrading enzymes (polygalactosidase, PG; ellulase, CX; pectin methylesterase, PME), as well as ethanol synthesis-related (alcohol dehydrogenase, ADH), acetaldehyde synthesis-related (pyruvate decarboxylase, PDC) and polyphenol decomposition-related genes (polyphenol oxidase, PPO). Moreover, the genes that are involved in ethylene (1-aminocyclopropane- 1-carboxylate oxidase, ACO; 1-aminocyclopropane- 1-carboxylate synthase, ACS) and abscisic acid (short-chain alcohol dehydrogenase, SDR; aldehyde oxidase, AAO) synthesis were significantly up-regulated. In addition, the bitter tasting amino acids, alkaloids and polyphenols were significantly increased in watercore tissue. Above all, these findings suggested that the metabolic disorder of sorbitol and sucrose can lead to an increase in plant hormones (abscisic acid and ethylene) and anaerobic respiration, resulting in aggravated fruit rot and the formation of bitter substances.

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Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Analyses Provide Insights into the Watercore Disorder on “Akibae” Pear Fruit

Liu¹,

Fan²,

Liu³

et al. 2021

IJMS

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“…The RNA-seq results by qRT-PCR were validated as indicated in [ 18 ]. The primers employed are shown in Table S20 .…”

Section: Methodsmentioning

confidence: 99%

“…The oil content and composition are determined by the cultivar with little environmental influence, whereas other compounds (phenols and sterols) depend more on the environment [ 6 ]. Because of the increasing oil content during fruit pulp growth, which can reach 30% (fresh weight) at full ripening [ 6 ], and due to the high commercial value of the oil, most studies to date have attempted to elucidate the molecular bases of olive fruit ripening [ 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]; however, to date, few studies have shown candidate genes associated with early olive fruit development [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. In particular, the transcriptomic control of changes associated with early fruit development in olive remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Transcriptome Dynamics during Early Fruit Development in Olive (Olea europaea L.)

Camarero

Briegas²,

Corbacho³

et al. 2023

IJMS

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In the olive (Olea europaea L.), an economically leading oil crop worldwide, fruit size and yield are determined by the early stages of fruit development. However, few detailed analyses of this stage of fruit development are available. This study offers an extensive characterization of the various processes involved in early olive fruit growth (cell division, cell cycle regulation, and cell expansion). For this, cytological, hormonal, and transcriptional changes characterizing the phases of early fruit development were analyzed in olive fruit of the cv. ‘Picual’. First, the surface area and mitotic activity (by flow cytometry) of fruit cells were investigated during early olive fruit development, from 0 to 42 days post-anthesis (DPA). The results demonstrate that the cell division phase extends up to 21 DPA, during which the maximal proportion of 4C cells in olive fruits was reached at 14 DPA, indicating that intensive cell division was activated in olive fruits at that time. Subsequently, fruit cell expansion lasted as long as 3 weeks more before endocarp lignification. Finally, the molecular mechanisms controlling the early fruit development were investigated by analyzing the transcriptome of olive flowers at anthesis (fruit set) as well as olive fruits at 14 DPA (cell division phase) and at 28 DPA (cell expansion phase). Sequential induction of the cell cycle regulating genes is associated with the upregulation of genes involved in cell wall remodeling and ion fluxes, and with a shift in plant hormone metabolism and signaling genes during early olive fruit development. This occurs together with transcriptional activity of subtilisin-like protease proteins together with transcription factors potentially involved in early fruit growth signaling. This gene expression profile, together with hormonal regulators, offers new insights for understanding the processes that regulate cell division and expansion, and ultimately fruit yield and olive size.

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“…For example, AZ cells undergo polarized oriented cell wall building activity, which culminates with a decrease in methylesterified homogalacturonan HG (JIM5 signal increases) during abscission in a polarized manner [23]. Furthermore, two gene candidates associated with vesicle trafficking were also identified, which could be involved in auxin related transport, secretion and/or recycling of cell-wall components as found in other abscission systems [80][81][82][83][84]. In addition, changes in the cation environment were also observed in the oil palm AZ during fruit abscission [23], while pH changes are important for AZ function during tomato flower abscission [85].…”

Section: Common Organ Abscission Genes and Processes Of Eudicots And mentioning

confidence: 99%

Multi-scale comparative transcriptome analysis reveals key genes and metabolic reprogramming processes associated with oil palm fruit abscission

et al. 2021

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Background Fruit abscission depends on cell separation that occurs within specialized cell layers that constitute an abscission zone (AZ). To determine the mechanisms of fleshy fruit abscission of the monocot oil palm (Elaeis guineensis Jacq.) compared with other abscission systems, we performed multi-scale comparative transcriptome analyses on fruit targeting the developing primary AZ and adjacent tissues. Results Combining between-tissue developmental comparisons with exogenous ethylene treatments, and naturally occurring abscission in the field, RNAseq analysis revealed a robust core set of 168 genes with differentially regulated expression, spatially associated with the ripe fruit AZ, and temporally restricted to the abscission timing. The expression of a set of candidate genes was validated by qRT-PCR in the fruit AZ of a natural oil palm variant with blocked fruit abscission, which provides evidence for their functions during abscission. Our results substantiate the conservation of gene function between dicot dry fruit dehiscence and monocot fleshy fruit abscission. The study also revealed major metabolic transitions occur in the AZ during abscission, including key senescence marker genes and transcriptional regulators, in addition to genes involved in nutrient recycling and reallocation, alternative routes for energy supply and adaptation to oxidative stress. Conclusions The study provides the first reference transcriptome of a monocot fleshy fruit abscission zone and provides insight into the mechanisms underlying abscission by identifying key genes with functional roles and processes, including metabolic transitions, cell wall modifications, signalling, stress adaptations and transcriptional regulation, that occur during ripe fruit abscission of the monocot oil palm. The transcriptome data comprises an original reference and resource useful towards understanding the evolutionary basis of this fundamental plant process.

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Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation among Olea europaea Fruit Tissues in Late Development

Cited by 14 publications

References 74 publications

Transcriptome and Metabolome Analyses Provide Insights into the Watercore Disorder on “Akibae” Pear Fruit

Transcriptome and Metabolome Analyses Provide Insights into the Watercore Disorder on “Akibae” Pear Fruit

Characterization of Transcriptome Dynamics during Early Fruit Development in Olive (Olea europaea L.)

Multi-scale comparative transcriptome analysis reveals key genes and metabolic reprogramming processes associated with oil palm fruit abscission

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