Abstract:Grape-derived proanthocyanidins could act as a protector against various environmental stresses for Saccharomyces cerevisiae during wine fermentation, resulting in the increased physiological activity, fermentation efficiency and improved wine quality. In order to explore the possible protection mechanism of proanthocyanidins globally, RNA-seq analysis for wine yeast AWRI R2 cultivated with 0 g/L (group A), 0.1 g/L (group B), 1.0 g/L (group C) proanthocyanidins were applied in this study. Differentially expres… Show more
“…Moreover, the floral, fruity, sweet, and overall acceptability attributes of the co-culture fermented ciders were notably enhanced, particularly in the case of LAM134 cider, suggesting that the addition of L. plantarum during simultaneous AF and MLF positively influenced cider acidity, consistent with prior studies ( Zhang et al, 2022 ). Furthermore, the sensory evaluation findings revealed notable discrepancies among the apple ciders fermented with S. cerevisiae and different strains of L. plantarum , underscoring the significance of strain-level differences in L. plantarum co-fermentation as a pivotal factor affecting the sensory qualities of the ciders, which aligns with previous research findings ( Li, Zhu, & Zhao, 2020 ). Fig.…”
“…Moreover, the floral, fruity, sweet, and overall acceptability attributes of the co-culture fermented ciders were notably enhanced, particularly in the case of LAM134 cider, suggesting that the addition of L. plantarum during simultaneous AF and MLF positively influenced cider acidity, consistent with prior studies ( Zhang et al, 2022 ). Furthermore, the sensory evaluation findings revealed notable discrepancies among the apple ciders fermented with S. cerevisiae and different strains of L. plantarum , underscoring the significance of strain-level differences in L. plantarum co-fermentation as a pivotal factor affecting the sensory qualities of the ciders, which aligns with previous research findings ( Li, Zhu, & Zhao, 2020 ). Fig.…”
“…1 and 3). These results are supported by similar observations for S. cerevisiae (Dong et al 2017;Li et al 2020). P. pastoris responds to lignocellulose-derived inhibitors by increasing oxidative stress response.…”
The negative effects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a significant drawback to the usage of biomass feedstocks for the production of fuels and chemicals. The yeast Pichia pastoris has shown a great biotechnological potential for producing heterologous proteins and renewable chemicals. Despite its relevance, the performance of P. pastoris in presence of lignocellulose-derived inhibitors remains unclear. In this work, our results show for the first time the dose-dependent response of P. pastoris to acetic acid, furaldehydes (HMF and furfural), and sugarcane biomass hydrolysate, both at physiological and transcriptional levels. The yeast was able to grow in synthetic media with up to 6 g.L−1 acetic acid, 1.75 g.L−1 furaldehydes or hydrolysate diluted to 10% (v/v). However, its metabolism was completely hindered in presence of hydrolysate diluted to 30% (v/v). Additionally, the yeast was capable to co-consume acetic acid and glucose. At the transcriptional level, P. pastoris response to lignocellulose-derived inhibitors relays on the up-regulation of genes related to transmembrane transport, oxidoreductase activities, RNA processing, and the repression of pathways related to biosynthetic processes and central carbon metabolism. These results demonstrate a polygenetic response that involves detoxification activities, and maintenance of energy and cellular homeostasis. In this context, ALD4, OYE3, QOR2, NTL100, YCT1, and PPR1 were identified as target genes to improve P. pastoris tolerance. Altogether, this work provides valuable insights into the P. pastoris stress tolerance, which can be useful to expand its use in different bioprocesses.
“…Traditional methods for extracting anthocyanins from plant tissues are limited by plant growth, arable land constraints, and operationally di cult extraction methods, making it very expensive to obtain anthocyanins. Although metabolic engineering with microbes is a very promising approach, there are still challenges associated with the large-scale production of anthocyanins [33]. In this study, we constructed the anthocyanin synthesis pathway in yeast using the S. cerevisiae platform technology and HR the anthocyanin synthase genes into the yeast genome to achieve de novo synthesis of anthocyanins.…”
Background Anthocyanins are water-soluble flavonoids in plants, which give plants bright colors and are widely used as food coloring agents, nutrients, and cosmetic additives. There are several limitations for traditional techniques of collecting anthocyanins from plant tissues, including species, origin, season, and technology. The benefits of using engineering microbial production of natural products include ease of use, controllability, and high efficiency.
Results In this study, ten genes of anthocyanins synthetic pathway were successfully cloned from anthocyanin-rich plant materials blueberry fruit and purple round eggplant rind. The Yeast Fab Assembly technology was utilized to construct the transcriptional units of these genes under different promoters. The transcriptional units of PAL and C4H, 4CL and CHSwere fused and inserted into Chr. XVI and IV of yeast strain JDY52 respectively using homologous recombination to gain Strain A. The fragments containing the transcriptional units of CHI and F3H, F3'H and DFRwere inserted into Chr. III and XVI to gain Strain B1. Strain B2 has the transcriptional units of ANS and 3GT in Chr. IV. Several anthocyanins, including cyanidin, peonidin, pelargonidin, petunidin, and malvidin, were detected by LC-MS/MS following the predicted outcomes of the de novo biosynthesis of anthocyanins in S. cerevisiae using a multi-strain co-culture technique.
ConclusionWe propose a novel concept for advancing the heterologous De novo anthocyanin synthesis pathway, as well as fundamental information and a theoretical framework for the ensuing optimization of the microbial synthesis of anthocyanins.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
