Transcriptome analysis reveals the proline metabolic pathway and its potential regulation TF-hub genes in salt-stressed potato

Abstract

Potato (Solanum tuberosum) is currently the third most important food crop in the world. However, the production of potato is seriously threatened by salt stress, which often occurs in the facility cultivation environment, and the mining of salt tolerance genes in potato remains to be further studied. In this study, test-tube plantlets of DM potato were treated with 200-mM NaCl to simulate salt stress, and 15 cDNA libraries were constructed for RNA-seq analysis. A total of 8383 DEGs were identified, of which 3961 DEGs were shared among all the salt treatments, and 264 (7.15%) TF-coding genes were identified from these shared DEGs. KEGG enrichment analysis showed that most DEGs identified from the “arginine and proline metabolism” (ko00330) were enriched in the proline metabolic pathway, and their functions almost covered the whole proline metabolic process. Further analysis showed that expression levels of all the 13 structural DEGs in the pathway were significantly up-regulated and proline accumulation was also significantly increased under salt stress, and 13 TF-hub genes were discovered by WGCNA in the lightcyan and tan modules which were highly positively correlated with the proline contents. Correlation analysis revealed that the four TF-hub genes of the lightcyan module and seven structural DEGs of the proline metabolic pathway might be the potential candidate genes, especially the potential and novel regulatory gene StGLK014720. Furthermore, the dual-luciferase reporter assay confirmed that the key protein StGLK014720 could activate the promoters of both structural genes StAST021010 and StAST017480. In conclusion, these results lay the foundation for further study on the salt tolerance mechanism of potato, and provide a theoretical basis and new genetic resources for salt tolerance breeding of potato.

Transcriptomic investigation unveils the role of energy metabolism under low phosphorus and salt combined stress in soybean (Glycine max)

“…The composition of all four treatments was based on 1/2 Hoagland nutrient solution, which included NH 4 H 2 PO 4 (0.5 mM), KNO 3 (2.5 mM), Ca(NO 3 ) 2 .4H 2 O (2.5 mM), MgSO 4 .7H 2 O (1 mM), H 3 BO 3 (23 μM), ZnSO 4 .7H 2 O (0.38 μM), CuSO 4 ·5H 2 O (0.16 μM), MnCl 2 .4H 2 O (4.5 μM), H 2 MoO 4 (0.2 μM), Fe‐EDTA (25 μM). The CK treatment solution retained the composition of 1/2 Hoagland nutrient solution; the LP treatment solution decreased the P concentration to 1/10 of the original concentration (Sung et al, 2015); the S treatment solution involved the addition of NaCl (200 mM) to the 1/2 Hoagland nutrient solution (Jing et al, 2022); LPS treatment solution also incorporated NaCl (200 mM) into the LP nutrient solution. Each treatment contains three replications.…”

Genome-Wide Identification and Expression Profiling Analysis of SWEET Family Genes Involved in Fruit Development in Plum (Prunus salicina Lindl)

“…Different letters were used to indicate significant differences ( p < 0.05). The ggcor package was used to perform the correlation test in the R circumstance [ 41 ].…”

A Review of Potato Salt Tolerance

“…At the proteomic level, the number of differentially regulated proteins in potatoes increased by about three-fold under salt stress, and the level of most highly abundant proteins increased, except for genes associated with photosynthesis. Jing et al [ 215 ] (2022) conducted transcriptome sequencing on potato DM plants and found 13 differentially expressed genes in the proline metabolism pathway that were correlated with increased proline content of DM plants.…”