Transcriptome analysis reveals light-induced anthocyanin synthesis candidate genes in rabbiteye blueberry (<i>Vaccinium ashei</i>: Reade)

Guo, Xiaolan; Wang, Delu; Shakeel, Muhammad

doi:10.1080/13102818.2021.1924078

Cited by 6 publications

(3 citation statements)

References 52 publications

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“…Yan et al [44] found that the anthocyanin content in fruits of four rabbiteye blueberry varieties, namely 'Powderblue', 'Brightwell', 'Baldwin' and 'Tifblue', increased with the increase of light intensity when different shading treatments were applied. Guo et al [45] also conducted a similar experimental treatment on rabbiteye blueberry 'Powderblue', and verified again that the anthocyanin content of its fruit increased with the increase of light intensity. Some studies have also shown that anthocyanin synthesis during apple flower development is controlled both by growth and light intensity [35].…”

Section: Discussionmentioning

confidence: 85%

Physiological response of anthocyanin synthesis to different light intensities in blueberry

An,

Tan,

Song

et al. 2023

PLoS ONE

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Fruit color is an important economic character of blueberry, determined by the amount of anthocyanin content. Anthocyanin synthesis within the blueberry fruits is significantly affected by light. To reveal the physiological response mechanism of anthocyanin synthesis in blueberry fruits in different light intensities, four light intensities (100% (CK), 75%, 50% and 25%) were set for the ’O’Neal’ southern highbush blueberry as the experimental material in our study. The relationship between endogenous hormones content, associated enzyme activities, and variations with the anthocyanin content in blueberry fruits under various light intensities during the white fruit stage (S1), purple fruit stage (S2), and blue fruit stage (S3) were studied. The results showed that adequate light could significantly promote anthocyanin synthesis in blueberry fruits (P < 0.05). Blueberry fruits had an anthocyanin content that was 1.76~24.13 times higher under 100% light intensity than it was under non-full light intensity. Different light intensities significantly affected the content of endogenous hormones and the activity of associated enzymes in anthocyanin synthesis pathway (P < 0.05). Among them, the JA (jasmonic acid) content and PAL (phenylalanine ammonia lyase) activity of fruits under 100% light intensity were 2.49%~41.83% and 2.47%~48.48% higher than those under other light intensity, respectively. And a significant correlation was found between the variations in anthocyanin content in fruits and the content or activities of JA, ABA (abscisic acid), ETH (ethylene), GA3 (gibberellin 3), IAA (indoleacetic acid), PAL, CHI (chalcone isomerase), DFR (dihydroflavonol reductase) and UFGT (UDP-glucose: flavonoid 3-glucosyltransferase) (P < 0.05). It indicated that 100% light intensity significantly promoted anthocyanin synthesis in blueberry fruits by affecting endogenous hormones content and associated enzyme activities in the anthocyanin synthesis pathway. This study will lay a foundation for further research on the molecular mechanism of light intensity regulating anthocyanin synthesis in blueberry.

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Section: Discussionmentioning

confidence: 85%

Physiological response of anthocyanin synthesis to different light intensities in blueberry

An,

Tan,

Song

et al. 2023

PLoS ONE

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“…In the early stages of anthocyanin synthesis, the expression patterns of related genes can be regulated by different light qualities and intensities, and this expression pattern is dramatically changed and accompanied by distinct species. For example, anthocyanin accumulation within the blueberry peel is significantly affected by light, the content of anthocyanin and expression level of genes related to anthocyanin synthesis pathway were significantly different under various light treatments including: intensity 100%, 50%, and 20% ( Guo et al., 2021 ). Moreover, the differences in the light qualities on the anthocyanin biosynthesis also were significant.…”

Section: Discussionmentioning

confidence: 99%

Integrated transcriptome and metabolome analysis reveals the anthocyanin biosynthesis mechanisms in blueberry (Vaccinium corymbosum L.) leaves under different light qualities

Zhang

et al. 2022

Front. Plant Sci.

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IntroductionBlueberry (Vaccinium corymbosum L.) is a popular fruit with an abundance of anthocyanins in its leaves and fruits. Light is one of the pivotal environmental elements that affects plant growth and development, but the regulatory mechanism between light quality and anthocyanin formation is poorly understood.MethodsAn integrated transcriptome and metabolome analysis was performed to investigate the effects of white (control), blue (B), red (R), and red/blue (60R/40B) light on blueberry growth and reveal the potential pathway controlling anthocyanin biosynthesis in blueberry leaves.ResultsThe anthocyanin content was significantly improved by the blue and red/blue light when compared with white light, whereas there was a significant reduction in the photosynthesis under the blue light, showing an inverse trend to that of anthocyanin accumulation. Transcriptomic analysis resulted in the assembly of 134,709 unigenes. Of these, 22 were differentially expressed genes (DEGs) that participate in the anthocyanin biosynthesis pathway, with the majority being significantly up-regulated under the blue light. Most of the photosynthesis-related genes that were down-regulated were expressed during anthocyanin accumulation. Targeted metabolome profiling identified 44 metabolites associated with anthocyanin biosynthesis. The contents of most of these metabolites were higher under blue light than the other light conditions, which was consistent with the transcriptome results. The integrated transcriptome and metabolome analysis suggested that, under blue light, leucoanthocyanidin dioxygenase (LDOX), O-methyltransferase (OMT), and UDP-glucose flavonoid glucosyltransferase (UFGT) were the most significantly expressed, and they promoted the synthesis of cyanidin (Cy), malvidin (Mv), and pelargonidin (Pg) anthocyanidins, respectively. The expression levels of dihydroflavonol 4-reductase (DFR) and OMT, as well as the accumulation of delphinidin (Dp), peonidin (Pn), and petunidin (Pt), were significantly increased by the red/blue light.DiscussionThe blue and red/blue lights promoted anthocyanin biosynthesis via inducing the expression of key structural genes and accumulation of metabolites involved in anthocyanin synthesis pathway. Moreover, there was a possible feedback regulating correlation between anthocyanin biosynthesis and photosynthesis under different light qualities in blueberry leaves. This study would provide a theoretical basis for elucidating the underlying regulatory mechanism of anthocyanin biosynthesis of V. corymbosum.

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“…The molecular mechanism of anthocyanin biosynthesis induced by light intensity has been reported in blueberry [8,22]. However, it is not clear how the light intensity affects the physiological mechanism of anthocyanin content by affecting the endogenous hormone contents and the key enzyme activities in the anthocyanin synthesis pathway.…”

Section: Introductionmentioning

confidence: 99%

Effects of Light Intensity on Endogenous Hormones and Key Enzyme Activities of Anthocyanin Synthesis in Blueberry Leaves

Tan

Zhang

et al. 2023

Horticulturae

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Plant anthocyanin is a secondary metabolite widely distributed in the roots, stems, leaves, flowers and fruits of plants, and its synthesis is significantly affected by light intensity. To reveal the physiological response mechanism of anthocyanin synthesis in blueberry leaves at different light intensities, four light intensities (100% (CK), 75%, 50% and 25%) were set for the ‘O’Neal’ southern highbush blueberry as the experimental material in our study. The relationship between endogenous hormone contents, key enzyme activities, and variations in the anthocyanin content in blueberry leaves under various light intensities during the white fruit stage (S1), purple fruit stage (S2) and blue fruit stage (S3) of fruit development were studied. The results showed that the anthocyanin content of blueberry leaves increased first and then decreased, and decreased first and then increased with the increase in light intensity and development stage, respectively. The appropriate light intensity could significantly promote the synthesis of anthocyanin, and the anthocyanin content in leaves treated with 75% light intensity was 1.09~4.08 times that of other light intensity treatments. The content or activities of gibberellin (GA3), indoleacetic acid (IAA), jasmonic acid (JA), abscisic acid (ABA), ethylene (ETH), phenylalanine ammonia lyase (PAL), chalcone isomerase (CHI), dihydroflavonol reductase (DFR) and UDP-glucose: flavonoid 3-glucosyltransferase (UFGT) were significantly or extremely significantly correlated with the content of anthocyanin in leaves. This indicated that light intensity significantly promoted anthocyanin synthesis in blueberry leaves by affecting endogenous hormone contents and key enzyme activities in the anthocyanin synthesis pathway. This study lays a foundation for further research on the molecular mechanism of light intensity regulating anthocyanin synthesis in blueberry leaves.

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Transcriptome analysis reveals light-induced anthocyanin synthesis candidate genes in rabbiteye blueberry (Vaccinium ashei: Reade)

Cited by 6 publications

References 52 publications

Physiological response of anthocyanin synthesis to different light intensities in blueberry

Physiological response of anthocyanin synthesis to different light intensities in blueberry

Integrated transcriptome and metabolome analysis reveals the anthocyanin biosynthesis mechanisms in blueberry (Vaccinium corymbosum L.) leaves under different light qualities

Effects of Light Intensity on Endogenous Hormones and Key Enzyme Activities of Anthocyanin Synthesis in Blueberry Leaves

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