Transcriptome analysis reveals differential gene expression associated with white spot syndrome virus resistance in the shrimp Litopenaeus vannamei fed on functional diets

Cabrera-Stevens, Monica Janeth; Sánchez‐Paz, Arturo; Mendoza–Cano, Fernando; Escobedo‐Fregoso, Cristina; Encinas‐García, Trinidad; Elizondo‐González, Regina; Peña‐Rodríguez, Alberto

doi:10.1016/j.aquaculture.2021.737434

Cited by 8 publications

(2 citation statements)

References 90 publications

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“…It was hypothesized that the genes in these pathways could contribute to fulfilling the high energy requirements for virus replication. Additionally, Cabrera-Stevens et al (2022) suggested that the overexpression of these genes in the pathways of glycolysis and oxidative phosphorylation may facilitate energy-dependent biological processes used by the host immune defense against WSSV.…”

Section: Discussionmentioning

confidence: 99%

“…On the question of which organ/tissue is appropriate to study transcriptomic responses of shrimps concerning functional diets, Cabrera-Stevens et al (2022) stated that the muscle of shrimp allows for novel insights about the physiological and nutritional status that improves resistance against viral infection and provides valuable insights on growth and nutrient metabolism, including a diversity of signaling pathways and immune-related pathways (Dai et al, 2017;Li et al, 2020). These same authors explained that hemocytes participate in humoral immunity by synthesizing and releasing immune molecules as antibacterial peptides, lectins, and crustins.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome profiling of Penaeus vannamei hepatopancreas infected with WSSV following feeding diet containing ulvan

Tumbokon

2022

Israeli Journal of Aquaculture - Bamidgeh

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The present study aimed to elucidate the mechanism of protection provided by dietary ulvan as observed in previous studies. Thirty shrimps (3.35 ± 0.08 g average body weight) were stocked in six polyethylene tanks (5 shrimps tank-1, treatments were in three replicate/tank) and were fed either a diet containing no ulvan (control) or containing ulvan at 1 g kg diet-1 for 35 days at 10% body weight twice daily. At the termination of the feeding, the shrimps were subjected to a white spot syndrome virus (WSSV) challenge test via intramuscular injection of the viral inoculum. Shrimps were sacrificed after 24 h of exposure, and the hepatopancreas was excised for total RNA extraction for transcriptome profiling. Biological validation of the RNA-seq results was also performed for 10 immune-related genes (6 up- and 4 down-regulated genes). A comparison of the ulvan group with the control group revealed that 69 differentially expressed genes (DEGs) were significantly up-regulated, whereas 640 were significantly down-regulated. 184 DEGs between the control and ulvan-treated groups were classified into six KEGG categories of Metabolism (145 DEGs), Organismal systems (16), Human disease (4), Genetic Information Processing (10), Cellular Processes (2), and Environmental Information Processing (7). The 145 DEGs under Metabolism were distributed to Level 2 subcategories as carbohydrate metabolism (59 DEGs), global and overview maps (44), energy metabolism (27), and amino acid metabolism (15). All candidate immune-related genes (67) were down-regulated except for 5 genes. The validation experiment showed proportionality of gene expressions of the qPCR and of those in the assembled transcriptome, justifying the acceptability of the RNA-seq results. In conclusion, data from the present study provided mechanisms for protecting the white shrimp by dietary ulvan against WSSV infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of Penaeus vannamei hepatopancreas infected with WSSV following feeding diet containing ulvan

Tumbokon

2022

Israeli Journal of Aquaculture - Bamidgeh

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Present Status, Limitations, and Prospects of Using Streptomyces Bacteria as a Potential Probiotic Agent in Aquaculture

Butt

Khan

Liu

et al. 2023

Probiotics & Antimicro. Prot.

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Streptomyces is a Gram-positive bacterium, belonging to the family Streptomycetaceae and order Streptomycetales. Several strains from different species of Streptomyces can be used to promote the health and growth of artificially cultured fish and shellfish by producing secondary metabolites including antibiotics, anticancer agents, antiparasitic agents, antifungal agents, and enzymes (protease and amylase). Some Streptomyces strains also exhibit antagonistic and antimicrobial activity against aquaculture-based pathogens by producing inhibitory compounds such as bacteriocins, siderophores, hydrogen peroxide, and organic acids to compete for nutrients and attachment sites in the host. The administration of Streptomyces in aquaculture could also induce an immune response, disease resistance, quorum sensing/antibiofilm activity, antiviral activity, competitive exclusion, modification in gastrointestinal microflora, growth enhancement, and water quality amelioration via nitrogen fixation and degradation of organic residues from the culture system. This review provides the current status and prospects of Streptomyces as potential probiotics in aquaculture, their selection criteria, administrative methods, and mechanisms of action. The limitations of Streptomyces as probiotics in aquaculture are highlighted and the solutions to these limitations are also discussed.

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Comprehensive evaluation of germplasm resources of nine Macrobrachium rosenbergii strains in China

Du,

Qi,

Lin

et al. 2023

Aquaculture Reports

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Transcriptome analysis reveals differential gene expression associated with white spot syndrome virus resistance in the shrimp Litopenaeus vannamei fed on functional diets

Cited by 8 publications

References 90 publications

Transcriptome profiling of Penaeus vannamei hepatopancreas infected with WSSV following feeding diet containing ulvan

Transcriptome profiling of Penaeus vannamei hepatopancreas infected with WSSV following feeding diet containing ulvan

Present Status, Limitations, and Prospects of Using Streptomyces Bacteria as a Potential Probiotic Agent in Aquaculture

Comprehensive evaluation of germplasm resources of nine Macrobrachium rosenbergii strains in China

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