Transcriptome analysis provides StMYBA1 gene that regulates potato anthocyanin biosynthesis by activating structural genes

Zhao, Xiaoyan; Zhang, Huiling; Liu, Tengfei; Zhao, Yanan; Hu, Xinxi; Liu, Shengxuan; Lin, Yuan; Song, Baoxing; He, Changzheng

doi:10.3389/fpls.2023.1087121

Cited by 4 publications

(3 citation statements)

References 50 publications

(58 reference statements)

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“…This compound is then converted by CHS, CHI, F3H, F3′H, and F3′5′H to the direct precursors of anthocyanin synthesis. Through DFR, colorless anthocyanins are formed and further catalyzed by ANS/LDOX to produce various colored anthocyanins; finally, UFGT catalyzes these molecules into stable anthocyanins (Bao et al, 2022;Zhao et al, 2023). Two important branches are present downstream of the phenylpropanoid metabolic pathway: the lignin synthesis pathway and the flavonoid synthesis pathway.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and metabolic analysis unveils the mechanism behind leaf color development in Disanthus cercidifolius var. longipes

Tian,

Xiang,

et al. 2024

Front. Mol. Biosci.

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Introduction: Leaf coloration in Disanthus cercidifolius var. longipes results from the interplay of various pigments undergoing complex catalytic reactions.Methods: We aimed to elucidate the mechanisms of pigment biosynthesis affecting leaf color transition in D. cercidifolius var. longipes by analyzing variations in pigment accumulation and levels of gene expression.Results: We identified 468, 577, and 215 differential metabolites in green leaves (GL), gradual-color-changing leaves (GCCL), and red leaves (RL), respectively, with 94 metabolites shared across all comparisons. Metabolite accumulation patterns were similar among GL, GCCL, and RL, with flavonoids being the main differential metabolites. Delphinidin, malvidin, and petunidin derivatives were mostly accumulated in GCCL, whereas cyanidin, pelargonidin, and peonidin derivatives accumulated in RL. Transcriptome sequencing was used to identify differentially expressed genes. The expression of anthocyanin biosynthetic pathway genes was associated with anthocyanin accumulation patterns.Discussion: Our findings reveal that the content of delphinidin, malvidin, petunidin, and carotenoids collectively determines the gradual transition of leaf color from green in spring and summer to green, purple, and orange-yellow in early autumn, whereas the content of cyanidin, peonidin, pelargonidin, and carotenoids together causes the autumnal transition to red or orange-red colors as leaves of D. cercidifolius var. longipes age.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic and metabolic analysis unveils the mechanism behind leaf color development in Disanthus cercidifolius var. longipes

Tian,

Xiang,

et al. 2024

Front. Mol. Biosci.

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“…Additionally, anthocyanin biosynthesis is regulated by the MBW transcription factor complex [11,13]. In potatoes, StAN1, StMYBA1, StMYB113, StJAF13, StbHLH1, and StAN11 have been proven to regulate anthocyanin biosynthesis, with StAN1, StMYBA1, and StMYB113 belonging to the R2R3-MYB TFs [7,[41][42][43], StJAF13 and StbHLH1 belonging to the bHLH TFs [7,44], and StAN11 being a WD40 protein [45]. In this study, enhanced UV-B radiation induced the up-regulation of StbHLH1 expression, while the other five TFs did not show significant differences, implying that the StbHLH1 TF was involved in UV-B radiationinduced tuber anthocyanin biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Enhanced UV-B Radiation in Potato Stems and Leaves Promotes the Accumulation of Anthocyanins in Tubers

Cui,

Li,

Zhang

et al. 2023

CIMB

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Enhanced ultraviolet-B (UV-B) radiation promotes anthocyanin biosynthesis in leaves, flowers and fruits of plants. However, the effects and underlying mechanisms of enhanced UV-B radiation on the accumulation of anthocyanins in the tubers of potatoes (Solanum tuberosum L.) remain unclear. Herein, reciprocal grafting experiments were first conducted using colored and uncolored potatoes, demonstrating that the anthocyanins in potato tubers were synthesized in situ, and not transported from the leaves to the tubers. Furthermore, the enhanced UV-B radiation (2.5 kJ·m−2·d−1) on potato stems and leaves significantly increased the contents of total anthocyanin and monomeric pelargonidin and peonidin in the red-fleshed potato ‘21-1’ tubers, compared to the untreated control. A comparative transcriptomic analysis showed that there were 2139 differentially expressed genes (DEGs) under UV-B treatment in comparison to the control, including 1724 up-regulated and 415 down-regulated genes. The anthocyanin-related enzymatic genes in the tubers such as PAL, C4H, 4CL, CHS, CHI, F3H, F3’5’H, ANS, UFGTs, and GSTs were up-regulated under UV-B treatment, except for a down-regulated F3’H. A known anthocyanin-related transcription factor StbHLH1 also showed a significantly higher expression level under UV-B treatment. Moreover, six differentially expressed MYB transcription factors were remarkably correlated to almost all anthocyanin-related enzymatic genes. Additionally, a DEGs enrichment analysis suggested that jasmonic acid might be a potential UV-B signaling molecule involved in the UV-B-induced tuber biosynthesis of anthocyanin. These results indicated that enhanced UV-B radiation in potato stems and leaves induced anthocyanin accumulation in the tubers by regulating the enzymatic genes and transcription factors involved in anthocyanin biosynthesis. This study provides novel insights into the mechanisms of enhanced UV-B radiation that regulate the anthocyanin biosynthesis in potato tubers.

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“…Liu et al rreported that StMYBA1 and StMYB113 promote anthocyanin biosynthesis in tobacco leaves [ 28 ]. Moreover, StAN1 can activate the promoter activity of structural genes involved in potato anthocyanin synthesis, such as StCHS , StCHI and StF3’H , to promote the accumulation of anthocyanins in potato leaves [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Screening and functional analysis of StMYB transcription factors in pigmented potato under low-temperature treatment

Chen,

Wu,

Dong

et al. 2024

BMC Genomics

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MYB transcription factors play an extremely important regulatory role in plant responses to stress and anthocyanin synthesis. Cloning of potato StMYB-related genes can provide a theoretical basis for the genetic improvement of pigmented potatoes. In this study, two MYB transcription factors, StMYB113 and StMYB308, possibly related to anthocyanin synthesis, were screened under low-temperature conditions based on the low-temperature-responsive potato StMYB genes family analysis obtained by transcriptome sequencing. By analyzed the protein properties and promoters of StMYB113 and StMYB308 and their relative expression levels at different low-temperature treatment periods, it is speculated that StMYB113 and StMYB308 can be expressed in response to low temperature and can promote anthocyanin synthesis. The overexpression vectors of StMYB113 and StMYB308 were constructed for transient transformation tobacco. Color changes were observed, and the expression levels of the structural genes of tobacco anthocyanin synthesis were determined. The results showed that StMYB113 lacking the complete MYB domain could not promote the accumulation of tobacco anthocyanins, while StMYB308 could significantly promote the accumulation involved in tobacco anthocyanins. This study provides a theoretical reference for further study of the mechanism of StMYB113 and StMYB308 transcription factors in potato anthocyanin synthesis.

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Transcriptome analysis provides StMYBA1 gene that regulates potato anthocyanin biosynthesis by activating structural genes

Cited by 4 publications

References 50 publications

Transcriptomic and metabolic analysis unveils the mechanism behind leaf color development in Disanthus cercidifolius var. longipes

Transcriptomic and metabolic analysis unveils the mechanism behind leaf color development in Disanthus cercidifolius var. longipes

Enhanced UV-B Radiation in Potato Stems and Leaves Promotes the Accumulation of Anthocyanins in Tubers

Screening and functional analysis of StMYB transcription factors in pigmented potato under low-temperature treatment

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