Transcriptome Analysis Provides Insights into Potentilla bifurca Adaptation to High Altitude

Tang, Xun; Li, Jinping; Liu, Likuan; Jing, Hui; Zuo, Wenming; Zeng, Yang

doi:10.3390/life12091337

Cited by 5 publications

(5 citation statements)

References 50 publications

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“…Iljinsk [ 24 ]. The most significantly differentially expressed top 50 genes in the high-altitude samples were derived from plants that responded to abiotic stress, such as peroxidase, superoxide dismutase protein, and the ubiquitin-conjugating enzyme, and the KEGG pathway was related to secondary metabolites, including phenylpropane and flavonoids, in intraspecific adaptation to high altitude in Potentilla bifurca L. [ 25 ]. Compared to the above research, our findings showed a different picture of DEGs.…”

Section: Discussionmentioning

confidence: 99%

Insights into the Adaptation to High Altitudes from Transcriptome Profiling: A Case Study of an Endangered Species, Kingdonia uniflora

Nong,

Luo,

Zhu

et al. 2023

Genes

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Kingdonia uniflora is an endangered alpine herb that is distributed along an altitudinal gradient. The unique traits and important phylogenetic position make K. uniflora an ideal model for exploring how endangered plants react to altitude variation. In this study, we sampled nine individuals from three representative locations and adopted RNA-seq technology to sequence 18 tissues, aiming to uncover how K. uniflora responded to different altitudes at the gene expression level. We revealed that genes that responded to light stimuli and circadian rhythm genes were significantly enriched in DEGs in the leaf tissue group, while genes that were related to root development and peroxidase activity or involved in the pathways of cutin, suberin, wax biosynthesis, and monoterpenoid biosynthesis were significantly enriched in DEGs in the flower bud tissue group. All of the above genes may play an important role in the response of K. uniflora to various stresses, such as low temperatures and hypoxia in high-altitude environments. Furthermore, we proved that the discrepancy in gene expression patterns between leaf and flower bud tissues varied along the altitudinal gradient. Overall, our findings provide new insights into the adaptation of endangered species to high-altitude environments and further encourage parallel research to focus on the molecular mechanisms of alpine plant evolution.

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Section: Discussionmentioning

confidence: 99%

Insights into the Adaptation to High Altitudes from Transcriptome Profiling: A Case Study of an Endangered Species, Kingdonia uniflora

Nong,

Luo,

Zhu

et al. 2023

Genes

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“…Six flavonoids were determined in the G. aleppicum herb extract as flavonols in the glycoside state. Depending on the flavonol structure of aglycone, they belonged to the quercetin (13,17,18) or kaempferol (14, 20, 21) groups. Quercetin-3-O-glucuronide (17), quercetin-3-O-glucoside (18), kaempferol-3-O-glucuronide (20), and kaempferol-3-O-glucoside (21) were successfully identified in the G. aleppicum herb using reference standards.…”

Section: Flavonoidsmentioning

confidence: 99%

“…Three benzoic acid derivatives (9, 10, and 13) were determined in the S. bifurca herb. 3,4-Dihydroxybenzoic acid 4-O-glucoside ( 9) and 1-O-p-hydroxybenzoic acid O-glucoside (13) were identified by comparing these with reference standards. Compound 10 was established as p-hydroxybenzoic acid O-hexoside owing to specific UV (274 nm) and mass spectral patterns with the loss of a hexose fragment (162 Da) and the presence of ions with m/z 137 corresponding to the hydroxybenzoic acid moiety.…”

Section: Benzoic Acid Derivativesmentioning

confidence: 99%

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Metabolites of Geum aleppicum and Sibbaldianthe bifurca: Diversity and α-Glucosidase Inhibitory Potential

2023

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α-Glucosidase inhibitors are essential in the treatment of diabetes mellitus. Plant-derived drugs are promising sources of new compounds with glucosidase-inhibiting ability. The Geum aleppicum Jacq. and Sibbaldianthe bifurca (L.) Kurtto & T.Erikss. herbs are used in many traditional medical systems to treat diabetes. In this study, metabolites of the G. aleppicum and S. bifurca herbs in active growth, flowering, and fruiting stages were investigated using high-performance liquid chromatography with photodiode array and electrospray ionization triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS/MS). In total, 29 compounds in G. aleppicum and 41 components in S. bifurca were identified including carbohydrates, organic acids, benzoic and ellagic acid derivatives, ellagitannins, flavonoids, and triterpenoids. Gemin A, miquelianin, niga-ichigoside F1, and 3,4-dihydroxybenzoic acid 4-O-glucoside were the dominant compounds in the G. aleppicum herb, while guaiaverin, miquelianin, tellimagrandin II2, casuarictin, and glucose were prevailing compounds in the S. bifurca herb. On the basis of HPLC activity-based profiling of the G. aleppicum herb extract, the most pronounced inhibition of α-glucosidase was observed for gemin A and quercetin-3-O-glucuronide. The latter compound and quercetin-3-O-arabinoside demonstrated maximal inhibition of α-glucosidase in the S. bifurca herb extract. The obtained results confirm the prospects of using these plant compounds as possible sources of hypoglycemic nutraceuticals.

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“…With the development of high-throughput sequencing technology, plant RNA-seq datasets can be obtained rapidly and comprehensively [ 24 , 25 ]. This technology is now widely used in transcriptome gene expression analysis [ 26 ], secondary metabolism studies of plants [ 27 , 28 ], disease resistance mechanisms [ 29 ], biocontrol [ 30 , 31 ], and so on. RNA-seq datasets can also be used to study the molecular function of specific genes and to screen for internal reference genes [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Candidate Reference Genes for Gene Expression Analysis in Wild Lamiophlomis rotata

Wang

Qian

Geng

et al. 2023

Genes

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Lamiophlomis rotata (Benth.) Kudo is a perennial and unique medicinal plant of the Qinghai–Tibet Plateau. It has the effects of diminishing inflammation, activating blood circulation, removing blood stasis, reducing swelling, and relieving pain. However, thus far, reliable reference gene identifications have not been reported in wild L. rotata. In this study, we identified suitable reference genes for the analysis of gene expression related to the medicinal compound synthesis in wild L. rotata subjected to five different-altitude habitats. Based on the RNA-Seq data of wild L. rotata from five different regions, the stability of 15 candidate internal reference genes was analyzed using geNorm, NormFinder, BestKeeper, and RefFinder. TFIIS, EF-1α, and CYP22 were the most suitable internal reference genes in the leaves of L. rotata from different regions, while OBP, TFIIS, and CYP22 were the optimal reference genes in the roots of L. rotata. The reference genes identified here would be very useful for gene expression studies with different tissues in L. rotata from different habitats.

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Transcriptome Analysis Provides Insights into Potentilla bifurca Adaptation to High Altitude

Cited by 5 publications

References 50 publications

Insights into the Adaptation to High Altitudes from Transcriptome Profiling: A Case Study of an Endangered Species, Kingdonia uniflora

Insights into the Adaptation to High Altitudes from Transcriptome Profiling: A Case Study of an Endangered Species, Kingdonia uniflora

Metabolites of Geum aleppicum and Sibbaldianthe bifurca: Diversity and α-Glucosidase Inhibitory Potential

Evaluation of Candidate Reference Genes for Gene Expression Analysis in Wild Lamiophlomis rotata

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