Transcriptome analysis of the response of silkworm to drastic changes in ambient temperature

Guo, Huizhen; Huang, Ching-Hua; Jiang, Liang; Cheng, Tingcai; Feng, Tieshan; Xia, Qingyou

doi:10.1007/s00253-018-9387-5

Cited by 24 publications

(8 citation statements)

References 48 publications

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“…These pathways might be involved in the response of the rice leaf folder larvae to heat stress. It has beeen found that the protein turnover, immune processes and signal transduction pathways had a close relationship with heat tolerance of Glyphodes pyloalis larvae [48]; several immune-related genes were downregulated when the fifth instar larvae of silkworm exposed to 37°C for 9 h [20]. In this study, furthermore, we found that the different KEGG pathways enriched for DEGs in the heatacclimated and unacclimated larvae when they were exposed to heat stress.…”

Section: Discussionsupporting

confidence: 59%

“…The increased expression levels of heat shock protein genes have been found in insects when exposed to heat stress [20,21,36,40]. Heat shock proteins, such as HSP40 and HSP70, protect insects from direct heat injuries [41,42].…”

Section: Discussionmentioning

confidence: 99%

“…Under high temperature conditions, evaporative cooling improves insect thermotolerance [18]. A general cellular response of insects to high temperature is the inductive heat shock proteins (Hsps) which protect insects from heat injuries [19,20]. For example, an upregulation of gene expression of the Hsp40 was found in thermotolerant lines of Drosophila melanogaster when they subjected to a mild heat shock [21].…”

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptome analysis of the rice leaf folder (Cnaphalocrocis medinalis) to heat acclimation

Quan

Wang

et al. 2020

BMC Genomics

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Background: The rice leaf folder Cnaphalocrocis medinalis Güenée is a serious insect pest of rice in Asia. This pest occurs in summer, and it is sensitive to high temperature. However, the larvae exhibit heat acclimation/adaptation. To understand the underlying mechanisms, we established a heat-acclimated strain via multigenerational selection at 39°C. After heat shock at 41°C for 1 h, the transcriptomes of the heat-acclimated (S-39) and unacclimated (S-27) larvae were sequenced, using the unacclimated larvae without exposure to 41°C as the control. Results: Five generations of selection at 39°C led larvae to acclimate to this heat stress. Exposure to 41°C induced 1160 differentially expressed genes (DEGs) between the heat-acclimated and unacclimated larvae. Both the heatacclimated and unacclimated larvae responded to heat stress via upregulating genes related to sensory organ development and structural constituent of eye lens, whereas the unacclimated larvae also upregulated genes related to structural constituent of cuticle. Compared to unacclimated larvae, heat-acclimated larvae downregulated oxidoreductase activity-related genes when encountering heat shock. Both the acclimated and unacclimated larvae adjusted the longevity regulating, protein processing in endoplasmic reticulum, antigen processing and presentation, MAPK and estrogen signaling pathway to responsed to heat stress. Additionally, the unacclimated larvae also adjusted the spliceosome pathway, whereas the heat-acclimated larvae adjusted the biosynthesis of unsaturated fatty acids pathway when encountering heat stress. Although the heat-acclimated and unacclimated larvae upregulated expression of heat shock protein genes under heat stress including HSP70, HSP27 and CRYAB, their biosynthesis, metabolism and detoxification-related genes expressed differentially. Conclusions: The rice leaf folder larvae could acclimate to a high temperature via multigenerational heat selection. The heat-acclimated larvae induced more DEGs to response to heat shock than the unacclimated larvae. The changes in transcript level of genes were related to heat acclimation of larvae, especially these genes in sensory organ development, structural constituent of eye lens, and oxidoreductase activity. The DEGs between heat-acclimated and unacclimated larvae after heat shock were enriched in the biosynthesis and metabolism pathways. These results are helpful to understand the molecular mechanism underlying heat acclimation of insects.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptome analysis of the rice leaf folder (Cnaphalocrocis medinalis) to heat acclimation

Quan

Wang

et al. 2020

BMC Genomics

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“…Dehydrogenases are a type of oxidoreductases that oxidize substrates by reducing an electron acceptor and are important for their role in detoxification of metabolites. Malate dehydrogenase (BmMDH1) and 6-phosphogluconate dehydrogenase (Bm6PGD) were previously found to be differentially expressed in Dazao silkworms exposed to different temperatures, which implies enhancement in energy metabolism and ATPase expression at low temperatures [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Global Profiling of Genes Expressed in the Silk Glands of Philippine-Reared Mulberry Silkworms (Bombyx mori)

Peña

Lao

Bautista

2022

Insects

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RNA sequencing was used to assemble transcriptome data for Philippine-reared silkworm and compare gene expression profiles of strains reared in high- and low-temperature environments. RNA was isolated from the silk glands of fifth instar larvae and mRNA-enriched libraries were sequenced using Illumina NextSeq 500. Transcriptome reads were assembled using reference-based and de novo assemblers, and assemblies were evaluated using different metrics for transcriptome quality, including the read mapping rate, E90N50, RSEM-eval, and the presence of single-copy orthologs. All transcriptome assemblies were able to reconstruct >40,000 transcripts. Differential expression analysis found 476 differentially expressed genes (DEGs; 222 upregulated, 254 downregulated) in strains reared in different temperatures. Among the top DEGs were myrosinase, heat shock proteins, serine protease inhibitors, dehydrogenases, and regulators of the juvenile hormone. Validation of some of the top DEGs using qPCR supported the findings of the in silico analysis. GO term enrichment analysis reveals an overrepresentation of GO terms related to nucleotide metabolism and biosynthesis, lipid and carbohydrate metabolic processes, regulation of transcription, nucleotide binding, protein binding, metal binding, catalytic activity, oxidoreductase activity, and hydrolase activity. The data provided here will serve as a resource for improving local strains and increasing silk production of Philippine-reared B. mori strains.

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“…Clean reads were mapped to the silkworm genome. The gene ontology (GO) analysis and KEGG annotation of the differentially expressed genes (DEGs) were executed using WEGO online and iPathCons, respectively (52, 60). PTEN was cloned using the BmPTEN ORF primer, and its expression after BmPGRP2-2 RNAi and overexpression was evaluated.…”

Section: Methodsmentioning

confidence: 99%

Distinct Functions of Bombyx mori Peptidoglycan Recognition Protein 2 in Immune Responses to Bacteria and Viruses

et al. 2019

Self Cite

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Peptidoglycan recognition protein (PGRP) is an important pattern recognition receptor in innate immunity that is vital for bacterial recognition and defense in insects. Few studies report the role of PGRP in viral infection. Here we cloned two forms of PGRP from the model lepidopteran Bombyx mori : BmPGRP2-1 is a transmembrane protein, whereas BmPGRP2-2 is an intracellular protein. BmPGRP2-1 bound to diaminopimelic acid (DAP)-type peptidoglycan (PGN) to activate the canonical immune deficiency (Imd) pathway. BmPGRP2-2 knockdown reduced B. mori nucleopolyhedrovirus (BmNPV) multiplication and mortality in cell lines and in silkworm larvae, while its overexpression increased viral replication. Transcriptome and quantitative PCR (qPCR) results confirmed that BmPGRP2 negatively regulated phosphatase and tensin homolog ( PTEN ). BmPGRP2-2 expression was induced by BmNPV, and the protein suppressed PTEN-phosphoinositide 3-kinase (PI3K)/Akt signaling to inhibit cell apoptosis, suggesting that BmNPV modulates BmPGRP2-2-PTEN-PI3K/Akt signaling to evade host antiviral defense. These results demonstrate that the two forms of BmPGRP2 have different functions in host responses to bacteria and viruses.

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Transcriptome analysis of the response of silkworm to drastic changes in ambient temperature

Cited by 24 publications

References 48 publications

Comparative transcriptome analysis of the rice leaf folder (Cnaphalocrocis medinalis) to heat acclimation

Comparative transcriptome analysis of the rice leaf folder (Cnaphalocrocis medinalis) to heat acclimation

Global Profiling of Genes Expressed in the Silk Glands of Philippine-Reared Mulberry Silkworms (Bombyx mori)

Distinct Functions of Bombyx mori Peptidoglycan Recognition Protein 2 in Immune Responses to Bacteria and Viruses

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