Transcriptome Analysis of Salt Stress in Hibiscus hamabo Sieb. et Zucc Based on Pacbio Full-Length Transcriptome Sequencing

Abstract

Hibiscus hamabo Sieb. et Zucc is an important semi-mangrove plant with great morphological features and strong salt resistance. In this study, by combining single molecule real time and next-generation sequencing technologies, we explored the transcriptomic changes in the roots of salt stressed H. hamabo. A total of 94,562 unigenes were obtained by clustering the same isoforms using the PacBio RSII platform, and 2269 differentially expressed genes were obtained under salt stress using the Illumina platform. There were 519 differentially expressed genes co-expressed at each treatment time point under salt stress, and these genes were found to be enriched in ion signal transduction and plant hormone signal transduction. We used Arabidopsis thaliana (L.) Heynh. transformation to confirm the function of the HhWRKY79 gene and discovered that overexpression enhanced salt tolerance. The full-length transcripts generated in this study provide a full characterization of the transcriptome of H. hamabo and may be useful in mining new salt stress-related genes specific to this species, while facilitating the understanding of the salt tolerance mechanisms.

Genome-Wide Identification and Analysis of MYB Transcription Factor Family in Hibiscus hamabo

“…As a semi-mangrove plant, H. hamabo has a high resistance to saline and alkaline soils and drought and barren environments [ 8 ]. Some Arabidopsis MYB transcription factors have been reported to be involved in plant resistance to salt and drought stresses [ 30 , 31 ].…”

“…H. hamabo is an excellent material for the study of plant salt tolerance. At present, studies on salt tolerance in H. hamabo have mainly been based on morphological observations: investigations of the physiological and molecular mechanisms involved [ 6 , 7 , 8 , 9 ]. Previously, we presented the genome sequencing of H. hamabo using PacBio, Illumina, and Hi-C sequencing technology and analyzed its salt tolerance mechanism based on genome and transcriptome data [ 2 ].…”

PacBio Full-Length Transcriptome Sequencing Reveals the Mechanism of Salt Stress Response in Sonneratia apetala

“…Several transcription factors, also known as cis-acting factors, have been reported to be associated with response to salt stress, which include MYB, WRKY, bHLH, bZIP and NAC [9]. Additionally, plants also respond to salt stress by regulating osmotic homeostasis [10]. For example, they increase the production of substances such as proline to regulate plant osmotic pressure [11].…”