Genome-Wide Identification and Analysis of MYB Transcription Factor Family in Hibiscus hamabo

Abstract

MYB transcription factors constitute one of the largest gene families in plants and play essential roles in the regulation of plant growth, responses to stress, and a wide variety of physiological and biochemical processes. In this study, 204 MYB proteins (HhMYBs) were identified in the Hibiscus hamabo Sieb. et Zucc (H. hamabo) genome and systematically analyzed based on their genomic sequence and transcriptomic data. The candidate HhMYB proteins and MYBs of Arabidopsis thaliana were divided into 28 subfamilies based on the analysis of their phylogenetic relationships and their motif patterns. Expression analysis using RNA-seq and quantitative real-time PCR (qRT-PCR) indicated that most HhMYBs are differentially regulated under drought and salt stresses. qRT-PCR analysis of seven selected HhMYBs suggested that the HhMYB family may have regulatory roles in the responses to stress and hormones. This study provides a framework for a more comprehensive analysis of the role of MYBs in the response to abiotic stress in H. hamabo.

Molecular Biology of Ornamental Plants

“…In addition, twenty RsAP2 genes were selected for a qRT-PCR, and the results showed that most of the RsAP2 genes responded to these abiotic stresses [12]. Liu et al analyzed 204 HhMYB family members in Hibiscus hamabo using RNA-seq and a qRT-PCR, and found that HhMYB was involved in plant responses to salt and drought stress to varying degrees [13]. Song et al identified and analyzed 25 SWEET genes in Rosa chinensis 'Old Blush', and through a transcriptome analysis, SWEET2a and SWEET10c were found to be candidate genes involved in the rose's ability to tolerate cold conditions [14].…”

Genome-Wide Identification of MYB Transcription Factors and Their Function on Floral Volatile Compounds Biosynthesis in Antirrhinum majus L.

“…The v-MYB avivan myoblastsis virus oncogene homolog (MYB) TFs family is the largest TFs family in plants working in plant growth and development, secondary metabolism and resistance to biotic and abiotic stress [4][5][6][7]. The first MYB was identified in the avian myoblast virus, and the first plant MYB was isolated from Zea mays [8,9]. Subsequently, researchers have continuously identified and elucidated the biological functions of MYB family members across various species [10][11][12].…”