Transcriptome analysis of porcine immature oocytes and surrounding cumulus cells after vitrification and in vitro maturation

Jia, Baoyu; Xiang, Decai; Quan, Guobo; Zhang, Bin; Shao, Qingyong; Hong, Qionghua; Wu, Guoquan

doi:10.1016/j.theriogenology.2019.05.019

Cited by 22 publications

(16 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, by using the Smart-seq2 technique, it was found that vitrification mainly affected apoptosis in mouse oocytes (Lee et al, 2019 ). In addition, Jia et al used the Smart-seq2 technique to study the effects of vitrification on the porcine oocyte transcriptome (Jia et al, 2019 ) and found that 37 genes were differentially expressed in vitrified GV oocytes after IVM. Currently, the effect of vitrification on transcriptomic profiles in porcine somatic cloned blastocysts is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Transcriptome Profiling Revealed That Vitrification of Somatic Cloned Porcine Blastocysts Causes Substantial Perturbations in Gene Expression

Zhang

Wei

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Single-Cell Transcriptome Profiling Revealed That Vitrification of Somatic Cloned Porcine Blastocysts Causes Substantial Perturbations in Gene Expression

Zhang

Wei

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

“…However, most of the epigenetic and expressional changes were observed from vitrification of immature oocytes (GV stage) followed by in vitro maturation step which is known to decrease subsequent embryo development in several mammals, including humans [127,133,147,148]. Furthermore, the effects of vitrification on epigenetic patterns and expression could vary in a manner dependent on species and gene and may also depend on the genomic regions analysed.…”

Section: Discussionmentioning

confidence: 99%

“…In a murine model, Gao et al found no differences between vitrified and fresh oocytes [125]. On the contrary, differentially expressed genes were found in bovine and porcine oocytes [126][127][128]. In particular, transcription regulation, cell differentiation and mitosis, regulation of actin cytoskeleton, and apoptosis pathways were found to undergo changes as a result of the oocyte vitrification process.…”

Section: Impact Of Oocyte Vitrification On Gene Expressionmentioning

confidence: 98%

See 1 more Smart Citation

What impact does oocyte vitrification have on epigenetics and gene expression?

Barberet¹,

Barry²,

Choux³

et al. 2020

Clin Epigenet

View full text Add to dashboard Cite

Children conceived by assisted reproductive technologies (ART) have a moderate risk for a number of adverse events and conditions. The question whether this additional risk is associated with specific procedures used in ART or whether it is related to the intrinsic biological factors associated with infertility remains unresolved. One of the main hypotheses is that laboratory procedures could have an effect on the epigenome of gametes and embryos. This suspicion is linked to the fact that ART procedures occur precisely during the period when there are major changes in the organization of the epigenome. Oocyte freezing protocols are generally considered safe; however, some evidence suggests that vitrification may be associated with modifications of the epigenetic marks. In this manuscript, after describing the main changes that occur during epigenetic reprogramming, we will provide current information regarding the impact of oocyte vitrification on epigenetic regulation and the consequences on gene expression, both in animals and humans. Overall, the literature suggests that epigenetic and transcriptomic profiles are sensitive to the stress induced by oocyte vitrification, and it also underlines the need to improve our knowledge in this field.

show abstract

“…Optimization of cell preservation techniques requires deep understanding of the processes happening in the cell in response to preservation. Several studies have used RNA sequencing (RNA-seq) to measure global transcriptomic changes in oocytes after vitrification followed by culturing or in vitro maturation [24][25][26][27][28], which provided informative transcriptomic data on the oocyte state after at least several hours post vitrification/ warming. Another recent study performed RNA-seq on mouse ovarian tissue after vitrification followed by 20 days of autotransplantation [29].…”

Section: Introductionmentioning

confidence: 99%

Initial response of ovarian tissue transcriptome to vitrification or microwave-assisted dehydration in the domestic cat model

Amelkina

Comizzoli

2020

BMC Genomics

View full text Add to dashboard Cite

Background Long term preservation of living ovarian tissues is a critical approach in human reproductive medicine as well as in the conservation of rare animal genotypes. Compared to single cell preservation, optimization of protocols for tissues is highly complex because of the diversity of cells responding differently to non-physiological conditions. Using the prepubertal domestic cat as a model, the objective was to study immediate effects of vitrification or microwave-assisted dehydration on the global transcriptome dynamics in the ovarian cortex. RNA sequencing was performed on ovarian tissues (n = 6 individuals) from different conditions: fresh tissue after dissection (F), vitrified/warmed tissue (V), tissue dehydrated for 5 min (D5) or 10 min (D10) followed by rehydration. Differential gene expression analysis was performed for comparison pairs V vs. F, D10 vs. F, D5 vs. F and D10 vs. D5, and networks were built based on results of functional enrichment and in silico protein-protein interactions. Results The impact of the vitrification protocol was already measurable within 20 min after warming and involved upregulation of the expression of seven mitochondrial DNA genes related to mitochondrial respiration. The analysis of D10 vs. F revealed, 30 min after rehydration, major downregulation of gene expression with enrichment of in silico interacting genes in Ras, Rap1, PI3K-Akt and MAPK signaling pathways. However, comparison of D5 vs. F showed negligible effects of the shorter dehydration protocol with two genes enriched in Ras signaling. Comparison of D10 vs. D5 showed downregulation of only seven genes. Vitrification and dehydration protocols mainly changed the expression of different genes and functional terms, but some of the differentially expressed genes formed a major in silico protein-protein interaction cluster enriched for mitochondrial respiration and Ras/MAPK signaling pathways. Conclusions Our results showed, for the first time, different effects of vitrification and microwave-assisted dehydration protocols on the global transcriptome of the ovarian cortex (using the domestic cat as a biomedical model). Acquired data and networks built on the basis of differentially expressed genes (1) can help to better understand stress responses to non-physiological stresses and (2) can be used as directions for future preservation protocol optimizations.

show abstract

Transcriptome analysis of porcine immature oocytes and surrounding cumulus cells after vitrification and in vitro maturation

Cited by 22 publications

References 61 publications

Single-Cell Transcriptome Profiling Revealed That Vitrification of Somatic Cloned Porcine Blastocysts Causes Substantial Perturbations in Gene Expression

Single-Cell Transcriptome Profiling Revealed That Vitrification of Somatic Cloned Porcine Blastocysts Causes Substantial Perturbations in Gene Expression

What impact does oocyte vitrification have on epigenetics and gene expression?

Initial response of ovarian tissue transcriptome to vitrification or microwave-assisted dehydration in the domestic cat model

Contact Info

Product

Resources

About