Plant intracellular immune receptors comprise a large number of multi-domain proteins resembling animal NOD-like receptors (NLRs). Plant NLRs typically recognize isolate-specific pathogen-derived effectors, encoded by avirulence (AVR) genes, and trigger defense responses often associated with localized host cell death. The barley MLA gene is polymorphic in nature and encodes NLRs of the coiled-coil (CC)-NB-LRR type that each detects a cognate isolate-specific effector of the barley powdery mildew fungus. We report the systematic analyses of MLA10 activity in disease resistance and cell death signaling in barley and Nicotiana benthamiana. MLA10 CC domain-triggered cell death is regulated by highly conserved motifs in the CC and the NB-ARC domains and by the C-terminal LRR of the receptor. Enforced MLA10 subcellular localization, by tagging with a nuclear localization sequence (NLS) or a nuclear export sequence (NES), shows that MLA10 activity in cell death signaling is suppressed in the nucleus but enhanced in the cytoplasm. By contrast, nuclear localized MLA10 is sufficient to mediate disease resistance against powdery mildew fungus. MLA10 retention in the cytoplasm was achieved through attachment of a glucocorticoid receptor hormone-binding domain (GR), by which we reinforced the role of cytoplasmic MLA10 in cell death signaling. Together with our data showing an essential and sufficient nuclear MLA10 activity in disease resistance, this suggests a bifurcation of MLA10-triggered cell death and disease resistance signaling in a compartment-dependent manner.
Study of intraflagellar transport protein IFT20 in male germ cell development shows that it is essential for male fertility and spermiogenesis in mice, playing a role in the building of sperm flagella and the disposal of cytoplasmic components.
Eastward transport and clockwise rotation of crust around the southeastern margin of the Tibetan Plateau dominates active deformation east of the Eastern Himalayan Syntaxis. Current crustal movement inferred from GPS measurements indicates ongoing distortion of the traces of the active Red River fault and the Mesozoic Yalong-Yulong-Longmen Shan thrust belt. By extrapolating current rates back in time, we infer that this pattern of deformation developed since 10.1 ± 1.5 Ma. This date of initiation is approximately synchronous with a suite of tectonic phenomena, both near and far, within the wide Eurasia/Indian collision zone, including the initiation of slip on the Ganzi-Yushu-Xianshuihe fault and crustal thinning and E-W extension by normal faulting on N-S−trending rifts in the plateau interior. Accordingly, the eastward movement of eastern Tibet and the clockwise rotation of that material seem to be local manifestations of a larger geodynamic event at ca. 10−15 Ma that changed the kinematic style and reorganized deformation not only on the plateau-wide scale, but across the entire region affected by the India/Eurasia collision. Convective removal of some or all of Tibet’s mantle lithosphere seems to offer the simplest mechanism for these approximately simultaneous changes.
In this study, we document the kinematics and Late Quaternary slip rates of actively developing faults and folds on the northern side of the Altyn Tagh Fault (ATF) that accommodate uplift and lateral expansion of the northern Tibetan Plateau. Field observations and detailed measurements using Unmanned-Aerial-Vehicle Structure-from-Motion high-resolution imagery of offset fan surfaces, gullies, and channel risers coupled with optically stimulated luminescence and 10 Be ages constrain the timing and slip rates of the Sanweishan Fault (SWSF) and Nanjieshan Fault (NJSF) systems. The NE striking SWSF is characterized by sinistral strike slip with a top-to-the-NW thrusting component. Offset geomorphic markers and dating results yield Pleistocene strike slip and vertical uplift rates of 0.06-1.25 mm/a and 0.05-0.08 mm/a, respectively. The E-W trending NJSF is dominated by north and south directed thrusting and km-scale folding with variable components of sinistral strike slip. The calculated total N-S shortening rate across the NJSF is~0.3 mm/a. Low rates of deformation for the SWSF and NJSF account for less than 10% of the total intraplate strain accommodated along the northeasternmost ATF system. Over a 1,000-km length, the northward expansion of the Tibetan Plateau occurs by progressive northeastward growth of a transpressional duplex rooted SE into the ATF. An assumed crustal strength discontinuity along the northeast trending southern margin of the Tarim Craton focuses oblique convergence along the ATF. Oblique-slip thrusting and sinistral strike slip along the ATF and to the north accommodate the oblique convergence, consistent with the ENE directed geodetically derived crustal velocity field driven by India's continued indentation 1,500 km to the south.
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