Transcriptome analysis of main roots of Panax notoginseng identifies genes involved in saponin biosynthesis under arsenic stress

Zu, Yanqun; Li, Zuran; Mei, Xinyue; Wu, Jiong; Cheng, Shicong; Jiang, Yueqin; Li, Yuan

doi:10.1016/j.plgene.2018.08.001

Cited by 17 publications

(6 citation statements)

References 24 publications

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“…Other depleted fatty acids, such as octadecenoic acids, can be found in root hairs of Glycine max (a relative of lupin within Fabaceae), where they are thought to be involved in interactions with symbiotic rhizobacteria (Brechenmacher et al, 2010). Saponins have also recently been identified in root exudates of Glycine max (Tsuno et al, 2018) and at high concentrations in roots of Panax notoginseng in arsenic contaminated soils (Zu et al, 2018). In addition, some saponins have been shown to protect mammalian cells from arsenic (Manna et al, 2007) and have been used as a biosurfactant to treat arsenic contaminated soils (Gusiatin, 2014).…”

Section: Discussionmentioning

confidence: 99%

Phytochelatin and coumarin enrichment in root exudates of arsenic‐treated white lupin

Frémont

Sas

Sarrazin

et al. 2021

Plant Cell & Environment

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Soil contamination with toxic metalloids, such as arsenic, can represent a substantial human health and environmental risk. Some plants are thought to tolerate soil toxicity using root exudation, however, the nature of this response to arsenic remains largely unknown. Here, white lupin plants were exposed to arsenic in a semihydroponic system and their exudates were profiled using untargeted liquid chromatography-tandem mass spectrometry. Arsenic concentrations up to 1 ppm were tolerated and led to the accumulation of 12.9 μg As g À1 dry weight (DW) and 411 μg As g À1 DW in above-ground and belowground tissues, respectively. From 193 exuded metabolites, 34 were significantly differentially abundant due to 1 ppm arsenic, including depletion of glutathione disulphide and enrichment of phytochelatins and coumarins. Significant enrichment of phytochelatins in exudates of arsenic-treated plants was further confirmed using exudate sampling with strict root exclusion. The chemical tolerance toolkit in white lupin included nutrient acquisition metabolites as well as phytochelatins, the major intracellular metal-binding detoxification oligopeptides which have not been previously reported as having an extracellular role. These findings highlight the value of untargeted metabolite profiling approaches to reveal the unexpected and inform strategies to mitigate anthropogenic pollution in soils around the world.

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Section: Discussionmentioning

confidence: 99%

Phytochelatin and coumarin enrichment in root exudates of arsenic‐treated white lupin

Frémont

Sas

Sarrazin

et al. 2021

Plant Cell & Environment

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“…It suggested that the mechanism of AMF affecting the ginsenosides synthesis in P. quinquefolius might be mainly through the activation of the MVA pathway. Studies had showed that the changes of genes involved in saponin biosynthesis were closely associated with the saponin content and composition (Zu et al 2018;Li et al 2016). Under the regulation of AMF, the key enzyme genes were e ciently transcribed and expressed in order to increase the accumulation of ginsenoside with high value, and ultimately improved the quality and economic value of P. quinquefolius.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Characterization of Panax Quinquefolius L. seedlings in Response to Arbuscular Mycorrhizal Fungi using RNA-seq

Yang

Zhang

et al. 2021

Preprint

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Panax quinquefolius L. has been considered as an important traditional Chinese medicine with a history of more than 300 years in China. Ginsenoside is the main bioactive component. Our research group has found that the accumulation of ginsenoside could be affected by arbuscular mycorrhizal fungi (AMF). However the underlying mechanism how AMF affected the biosynthesis of ginsenoside in P. quinquefolius is still unclear. In this study, the RNA-seq analysis was used to evaluate the effects of AMF (Rhizophagus intraradices, R. intraradices) on the expression of ginsenoside synthesis related genes in P. quinquefolius root. The results indicated that a symbiotic relationship between R. intraradices and P. quinquefolius was established. RNA-seq achieved approximately 48.62 G reads of all samples. Assembly of all the reads involved in all samples produced 63420 transcripts and 24137 unigenes. Differential expression analysis was performed between the control and AMF group. A total of 111 differentially expressed genes (DEGs) in response to AMF vs control were identified, 78 and 33 transcripts were upregulated and downregulated, respectively. Based on the functional analysis, Gene ontology (GO) analysis revealed that most DEGs were related to stress responses and cellular metabolic processes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis identified transduction, plant hormone signal transduction and terpenoids and polyketides biosynthesis pathways. Furthermore, the expression of glycolysis-related genes and ginsenoside synthesis related genes was largely induced by AMF. In conclusion, our results comprehensively elucidated the molecular mechanism how AMF affected the biosynthesis of ginsenoside in P.quinquefolius by transcriptome profiling.

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“…Saponin is a dammarane-type tetracyclic triterpene whose synthesis pathway includes the mevalonate pathway and the 2C-methyl-4-phosphate-4D-erythritol pathway [45,46]. The mevalonate pathway is dominant, which is co-catalyzed by a number of enzymes.…”

Section: Enzymology and Proteomics Mechanisms Of The Metabolism Of Saponin Under CD Stress With Lime Applicationmentioning

confidence: 99%

Effects of Lime Application on Activities of Related Enzymes and Protein Expression of Saponin Metabolism of <i>Panax notoginseng</i> under Cadmium Stress

Li¹,

Jiang²,

Zu³

et al. 2020

Pol. J. Environ. Stud.

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The soil Cd pollution has become one of the most important environmental problems [1]. Due to a series of activities of humans, including irrigation of farmland sewage and industrial wastes, the contents of heavy metals in the soil increased [2][3][4]. Cadmium in soil could be absorbed by plants, which affects plant growth and physiological metabolism and reduces the yield and quality of crops [5,6] . Plants reduce the toxic effects of heavy metals by altering their own structure

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Transcriptome analysis of main roots of Panax notoginseng identifies genes involved in saponin biosynthesis under arsenic stress

Cited by 17 publications

References 24 publications

Phytochelatin and coumarin enrichment in root exudates of arsenic‐treated white lupin

Phytochelatin and coumarin enrichment in root exudates of arsenic‐treated white lupin

Transcriptome Characterization of Panax Quinquefolius L. seedlings in Response to Arbuscular Mycorrhizal Fungi using RNA-seq

Effects of Lime Application on Activities of Related Enzymes and Protein Expression of Saponin Metabolism of <i>Panax notoginseng</i> under Cadmium Stress

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