Transcriptome Analysis Identifies Novel Mechanisms Associated with the Antitumor Effect of Chitosan-Stabilized Selenium Nanoparticles

Abstract

Selenium nanoparticles (SeNPs) have been receiving special attention in recent years due to their antioxidant capacity and antitumor properties. However, the mechanisms associated with these properties remain to be elucidated. For this reason, a global transcriptome analysis has been designed in this work and it was carried out using human hepatocarcinoma cells and chitosan-stabilized SeNPs (Ch-SeNPs) to identify new targets and pathways related to the antitumor mechanisms associated with Ch-SeNPs. The results obtained confirm the alteration of the cell cycle and the effect of Ch-SeNPs on different tumor suppressors and other molecules involved in key mechanisms related to cancer progression. Furthermore, we demonstrated the antioxidant properties of these nanoparticles and their capacity to induce senescence, which was further confirmed through the measurement of β-galactosidase activity.

Production of Cardamine violifolia selenium-enriched peptide using immobilized Alcalase on Fe3O4 modified by tannic acid and polyethyleneimine

“…As a necessary micronutrient for human health, Se has the effects of delaying aging, enhancing immune function, and preventing cancer. [1][2][3] A deciency in Se intake apparently affects the function and metabolism of gut microbes and even causes certain diseases such as Keshan disease. 4 Therefore, Se supplements in daily life have become a hotspot in nutrition research.…”

Selenium nanoparticles reduce cardiomyocyte apoptosis in Ascites Syndrome in Broiler Chickens via the ATF6-DR5 signaling pathway

“…Furthermore, endoplasmic reticulum stress is linked to a variety of cardiovascular diseases [6]. Selenium (Se), an essential trace element in humans and animals, is involved in numerous important physiological processes and exerts biological effects in the form of selenoproteins [25]. Selenoproteins are powerful antioxidants that play a role in immune enhancement and the prevention of cardiovascular disease [26].…”

A Multi-Omics Approach to Evaluate the Toxicity Mechanisms Associated with Silver Nanoparticles Exposure

“…This technology is based on the extraction of the mRNA, which is transformed into cDNA, which, in turn, is labeled with a fluorescence probe and then hybridized with its corresponding target [ 17 , 18 ]. Therefore, the integration of both techniques allows for the elucidation of the status of different metabolic pathways and, thus, provides a better understanding of the biological processes that are involved after a cytotoxic insult [ 19 , 20 ].…”