Transcriptional variants of Dmrt1 and expression of four Dmrt genes in the blunt snout bream, Megalobrama amblycephala

Su, Li-Na; Zhang, Fengjuan; Ding, Zhujin; Gao, Zexia; Wen, Jiufu; Wei, Wei; Wang, Qijun; Wang, Weimin; Liu, Hong

doi:10.1016/j.gene.2015.07.044

Cited by 20 publications

(15 citation statements)

References 52 publications

(72 reference statements)

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“…The evolutionary relationships of the dmrt genes in fish were subsequently examined using both phylogenetic and synteny analyses. Zhou et al (2008) showed that unlike mammals and other groups that only harbored one dmrt2, zebrafish carries a second paralog of dmrt2(2a), dmrt2b, which was subsequently identified in many other fish species (Zhou et al, 2008;Liu et al, 2009;Su et al, 2015;Lyu et al, 2019). The 17 representative fish species analyzed in the present study belong to Actinopterygii, with the exception of coelacanth L. chalumnae that belongs to Sarcopterygii.…”

Section: Discussionmentioning

confidence: 61%

“…The most conserved were shown in red fonts. (Fajkowska et al, 2016); A. japonica (Jeng et al, 2019); Acanthopagrus schlegelii (Wu and Chang, 2018); Anoplopoma fimbria (Smith et al, 2013); C. semilaevis (Cui et al, 2017); D. rerio (Lin et al, 2017;Webster et al, 2017); Epinephelus coioides (Lyu et al, 2019); G. morhua (Johnsen and Andersen, 2012); Gobiocypris rarus (Cao et al, 2012); Halichoeres poecilopterus (Miyake et al, 2012); L. chalumnae (Forconi et al, 2013); M. amblycephala (Su et al, 2015); M. salmoides (Yan et al, 2019); O. latipes and X. maculatus (Kondo et al, 2002); O. niloticus (Wei et al, 2019); Odontesthes bonariensis (Fernandino et al, 2008); O. mykiss (Marchand et al, 2000); Plecoglossus altivelis (Wang et al, 2014); Sebastes schlegeli (Ma et al, 2014); Solea senegalensis (Portela-Bens et al, 2017); T. rubripes (Yamaguchi et al, 2006); X. maculatus (Veith et al, 2006b) dmrt2 (dmrt2, 2a) 15 C. semilaevis (Zhu et al, 2019); Carassius auratus (Jiang et al, 2012); Carassius auratus gibelio (Liu and Gui, 2011); D. rerio (Zhou et al, 2008;Lu et al, 2017); G. morhua (Johnsen and Andersen, 2012); E. coioides (Lyu et al, 2019); Labeo rohita (Sahoo et al, 2019); M. albus (Sheng et al, 2014); M. amblycephala (Su et al, 2015); O. latipes and X. maculatus (Kondo et al, 2002); S. senegalensis (Portela-Bens et al, 2017); Scophthalmus maximus…”

Section: Discussionmentioning

confidence: 99%

“…The genomes of amphibians, reptiles, birds, and mammals contain only a single dmrt2 gene, whereas fish harbor two dmrt2 genes (dmrt2a and dmrt2b) (Liu et al, 2009;Su et al, 2015;Lyu et al, 2019). DMRT2 is widely distributed in the tissues of mammals and fish, and is expressed in both testes and ovaries (Kim et al, 2003;Winkler et al, 2004;El-Mogharbel et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Genomics Studies on the dmrt Gene Family in Fish

Dong

et al. 2020

Front. Genet.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Genomics Studies on the dmrt Gene Family in Fish

Dong

et al. 2020

Front. Genet.

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“…The Dmrt gene family is a group of genes with essential roles in animal sex differentiation (Su et al, ; Teaniniuraitemoana et al, ). While these genes have been widely studied in insects, nematodes and vertebrates (Hong et al, ; Vinas et al, ; Zarkower, ), information on the molecular characteristics and functions of Dmrt genes in crustaceans is still limited (Yang et al, ; Yu et al, , ).…”

Section: Discussionmentioning

confidence: 99%

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

et al. 2019

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Macrobrachium rosenbergii is an important aquaculture species known as the king of freshwater prawn. Macrobrachium rosenbergii displays remarkable sexual dimorphism, yet our knowledge of the molecular mechanism underpinning sex determination and differentiation of M. rosenbergii is still fragmentary. In this study, through in silico prediction and experimental characterization, we identified a new Dmrt gene (Mr-Dsx) from M. rosenbergii. The cDNA of Mr-Dsx included a 115 bp 5′ untranslated region (UTR), a 2,568 bp open reading frame (ORF) and a 259 bp 3′ UTR. Mr-Dsx was expressed in a wide range of tissues, and showed dynamic expression changes at different moulting stages in the testis, androgenic gland (AG) and eyestalk. In response to unilateral eyestalk ablation (UEA), Mr-Dsx was significantly induced in the testis and AG. Gene knockdown of Mr-Dsx resulted in pronounced suppression of the insulin-like androgenic hormone (IAG) gene. The results suggest that Mr-Dsx is likely to be negatively regulated by the eyestalk neurohormones, and Mr-Dsx may participate in the transcriptional activation of IAG. The study characterized the potential roles of a newly identified Dmrt homologue from M. rosenbergii, and would contribute to a deeper understanding of the molecular regulatory network underlying sex differentiation and moulting of prawns. K E Y W O R D S crustacean, Dmrt, Macrobrachium rosenbergii, moulting, sexual differentiation

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“…It is also expressed in MSG and medium size testis (Fig b). Su et al, (2015) has found higher expression of dmrt2a/terra in ovary than the testis in the adult stage and during gonadogenesis the expression level is vice versa in blunt snout bream Megalobrama amblycephala. In the platyfish, prominent expression of dmrt2a/terra was observed in the gills and is detectable in the gonads of both sexes in medaka, fugu and zebrafish (Veith et al, 2006).…”

Section: Dmrt2a5mentioning

confidence: 99%

Dimorphic Expression of Selected Genes in Lepidocephalus thermalis using Cross-Specific Primers

Balaganesan

Karalmarx

Shakila

2020

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Background: The existence of two distinct forms within a species that differ in one or more characteristics is known as dimorphism. The gonads are the primary organs in teleost to show sexual dimorphism. Lepidocephalus thermalis belongs to the Cobitidae family. No expression study on the developmental stages was done on this species. Since there is no specific primers reported for L. thermalis, the study has been carried out with the help of the specific primers of catfish. Methods: qRT- PCR is an acknowledged method for gene expression analysis due to its precision and reproducibility. In the current study, the expression of 14 different transcription factors involved in sex differentiation of Indian spiny loach during different developmental stages was analyzed using qRT- PCR and has been compared among the different stages of gonadal development for that transcription factor. Results: Gene expression patterns have been obtained from the total RNA isolated from MSG (Meso nephric gonadal complex), medium stage ovary, medium stage testis, large stage ovary and large stage testis. From the study, it has been analyzed that only sf1 has higher expression in testis and all the other transcription factors has shown higher expression in ovary.

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Transcriptional variants of Dmrt1 and expression of four Dmrt genes in the blunt snout bream, Megalobrama amblycephala

Cited by 20 publications

References 52 publications

Comparative Genomics Studies on the dmrt Gene Family in Fish

Comparative Genomics Studies on the dmrt Gene Family in Fish

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

Dimorphic Expression of Selected Genes in Lepidocephalus thermalis using Cross-Specific Primers

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