Potential involvement of a
 DMRT
 family member (
 Mr‐Dsx
 ) in the regulation of sexual differentiation and moulting in the giant river prawn
 Macrobrachium rosenbergii

Zhong, Ping; Zhou, Tingting; Yan, Zhang; Chen, Yujia; Yi, Jun-Qiao; Lin, Wen‐Yi; Guo, Zhen-Cong; Xu, Ai-Xuan; Yang, Shiping; Chan, S. C.; Wang, Wei

doi:10.1111/are.14262

Cited by 17 publications

(27 citation statements)

References 56 publications

(94 reference statements)

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“…Even in isopods, in which an elevated bacterial dosage of Wolbachia resulted in feminization, it was postulated that Wolbachia feminized males through intervention in the IAGswitch by instigating dysfunctional insulin receptors (Herran et al, 2020). However, while few other genes were found to be associated with the expression of IAG [MIH, GIH, CHH, CFSH, DMRT, and GEM (Li et al, 2015;Jin et al, 2019;Zhong et al, 2019;Jiang et al, 2020)], genes (other than the IAG itself) that act directly within the core of the IAG-switch cascade, have yet to be found.…”

Section: Introductionmentioning

confidence: 99%

The IAG-Switch and Further Transcriptomic Insights Into Sexual Differentiation of a Protandric Shrimp

et al. 2020

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The insulin-like androgenic gland hormone (IAG) secreted from the androgenic gland (AG) is a unique endocrine controller (the IAG-switch) of crustacean sexual differentiation. However, while previous studies of the IAG-switch focused mainly on sexual differentiation at early developmental stages of gonochoristic species, this mechanism is yet to be deciphered during naturally occurring sexual shifts in hermaphrodite species. The Northern spot shrimp, Pandalus platyceros, is a protandric hermaphrodite species, native to the North Pacific Ocean. We collected four stages of spot shrimp in Southeast Alaska including: juveniles, adult males, transitionals, and adult females. The AG was dissected from each stage and characterized histologically. Additionally, the IAG mRNA was sequenced. The function of the IAG-switch during the life history of this protandric species was demonstrated through monitoring IAG gene expression. Transcript levels were highest at the juvenile stage, then decreased significantly in mature males and became negligible in the transitional and female stages. Moreover, manipulating the IAG-switch via IAG loss of function in males through RNAi, induced the expected masculine to feminine sexual transformation that naturally occurs in this species. This included reduction in IAG transcript levels in males, elevation of vitellogenin gene expression in hepatopancreas and transformation of the gonad from an ovotestis containing both ovarian and testicular tissue to a true ovary with vitellogenic oocytes. Furthermore, a transcriptomic library from tissues associated with the endocrine axis upstream and downstream the IAG-switch yielded 1,801, 1,707, 1,946, and 182 differentially expressed genes between males, transitionals, and females in the AG, eyestalk, gonad, and hepatopancreas, respectively. Among these genes, the transcriptional pattern of six of them (all of them in the AG), between males, transitionals and females, had similar or inverted transcriptional pattern to that of IAG in P. platyceros. Five of these putatively IAG-switch associated genes are downregulated and one is upregulated throughout the P. platyceros shift from maleness to femaleness. Homolog protein sequences for the above novel genes were found in 17 other decapod species suggesting that they might represent conserved factors associated with the IAG-switch and involved in universal crustacean sexual differentiation mechanisms.

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Section: Introductionmentioning

confidence: 99%

The IAG-Switch and Further Transcriptomic Insights Into Sexual Differentiation of a Protandric Shrimp

et al. 2020

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“…This review serves as an outline reference for endocrine-driven sex determination and/or sexual differentiation systems in Malacostraca and Branchiopoda crustaceans. Although the transcriptional regulatory mechanisms between IAG and Dmrt genes have been investigated by gene knockdown approaches [ 161 , 162 ], the eyestalk (XO-SG complex)-derived neuropeptides that regulate IAG expression and those molecular networks are still largely unknown. Moreover, even among Malacostraca species, previous findings have so far demonstrated that endocrine systems vary in certain respects such as heterodimeric disulfide bond patterns of IAG.…”

Section: Discussionmentioning

confidence: 99%

Sex Determination and Differentiation in Decapod and Cladoceran Crustaceans: An Overview of Endocrine Regulation

Toyota

Miyakawa

Hiruta

et al. 2021

Genes

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Mechanisms underlying sex determination and differentiation in animals are known to encompass a diverse array of molecular clues. Recent innovations in high-throughput sequencing and mass spectrometry technologies have been widely applied in non-model organisms without reference genomes. Crustaceans are no exception. They are particularly diverse among the Arthropoda and contain a wide variety of commercially important fishery species such as shrimps, lobsters and crabs (Order Decapoda), and keystone species of aquatic ecosystems such as water fleas (Order Branchiopoda). In terms of decapod sex determination and differentiation, previous approaches have attempted to elucidate their molecular components, to establish mono-sex breeding technology. Here, we overview reports describing the physiological functions of sex hormones regulating masculinization and feminization, and gene discovery by transcriptomics in decapod species. Moreover, this review summarizes the recent progresses of studies on the juvenile hormone-driven sex determination system of the branchiopod genus Daphnia, and then compares sex determination and endocrine systems between decapods and branchiopods. This review provides not only substantial insights for aquaculture research, but also the opportunity to re-organize the current and future trends of this field.

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“…The insulin-like androgenic gland hormone (IAG) secreted from androgenic gland (AG) is a key regulator in sexual differentiation. Recent studies have shown that IAG itself is controlled by several genes and hormonal factors (Shen et al, 2014;Shi L. et al, 2019;Zhong et al, 2019). The detailed mechanisms and the role of sex-related genes and miRNAs in the IAG regulation are discussed in detail in other sections of the present review.…”

Section: Sexual Differentiation and Maturationmentioning

confidence: 91%

“…The Dmrt gene family plays essential roles in sexual differentiation. This gene family has been widely studied in vertebrates, nematodes, and insects, however, limited information is available regarding the molecular function of the Dmrt gene family in decapods (Zhong et al, 2019). To date, Dmrt has been isolated from different crustacean species including E. sinensis (Zhang and Qiu, 2010), the water flea D. magna (Toyota et al, 2013), S. verreauxi (Chandler et al, 2015), F. chinensis (Li et al, 2012b)…”

Section: Dmrt Gene Familymentioning

confidence: 99%

“…Recently, several sexual genes have been identified to be involved in the "eyestalk-IAG-testis" endocrine axis (Guo et al, 2019). It has been revealed that some of these genes are regulated by the eyestalk neurohormones and are located at the upstream of IAG and the sexual differentiation cascade (Shen et al, 2014;Ma et al, 2016a;Shi X. et al, 2019;Zheng et al, 2019;Zhong et al, 2019).…”

Section: Regulatory Mechanism Of Sex In Male Decapodsmentioning

confidence: 99%

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The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

et al. 2021

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Crustacean culture has been developing rapidly in various parts of the world. Therefore, it is important to understand their reproductive biology. Insulin-like androgenic gland hormone (IAG) secreted from the androgenic gland (AG) is widely accepted as a key regulator of sexual differentiation in male crustaceans. However, recently several sex-related genes (i.e., CFSH, DEAD-box family, Tra-2, Sxl, Dsx, Fem-1, Sox gene family, Foxl2, and Dmrt gene family) have been identified via transcriptomic analysis in crustaceans, indicating that sexual differentiation in crustaceans is more complicated than previously expected. It has been found that several non-coding RNAs (i.e., miRNAs, lncRNAs, and piRNAs) and IAG receptors may be involved in the sexual development of decapods. Identification and study of the regulation mechanism of sex-related genes, non-coding RNAs, and IAG receptors will provide valuable information regarding sexual development in decapods. In this review, the roles of hormonal and genetic factors in both males and females are discussed. In males, crustacean female sex hormone (CFSH), Sxl, Dmrt gene family, Dsx, Sox gene family, GEM, Fem-1, l-GnRH-III, and corazonin play important roles in IAG regulation in the “eyestalk-IAG-testis” endocrine axis. Unlike males, the regulation mechanism and interaction of sexual genes are relatively unknown in females. However, CFSH, IAG, Fem-1, FAMeT, Slo, UCHLs, Erk2, Cdc2, EGFR, Vg, VgR, and VIH seem to play crucial roles during ovarian development. This study summarizes the available information in the field, highlights gaps, and lays the foundations for further studies and a better understanding of the regulatory mechanism of sexual development in decapods.

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Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

Cited by 17 publications

References 56 publications

The IAG-Switch and Further Transcriptomic Insights Into Sexual Differentiation of a Protandric Shrimp

The IAG-Switch and Further Transcriptomic Insights Into Sexual Differentiation of a Protandric Shrimp

Sex Determination and Differentiation in Decapod and Cladoceran Crustaceans: An Overview of Endocrine Regulation

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

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