Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness

Minina, Elena A.; Moschou, Panagiotis N.; Vetukuri, Ramesh R.; Sánchez-Vera, Victoria; Cardoso, Catarina; Liu, Qinsong; Elander, Pernilla H.; Dalman, Kerstin; Beganovic, Mirela; Yilmaz, Jenny Lindberg; Marmon, Sofia; Shabala, Lana; Suárez, María Fernanda; Ljung, Karin; Novák, Ondřej; Shabala, Sergey; Stymne, Sten; Hofius, Daniel; Bozhkov, Peter V.

doi:10.1093/jxb/ery010

Cited by 134 publications

(155 citation statements)

References 81 publications

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“…Similar defects are seen with the atg4, atg5, atg7, and atg12 mutants under carbon starvation (Phillips et al, 2008;Chung et al, 2010). In contrast, in Arabidopsis, overexpression of ATG5 and ATG7 stimulates the lipidation of ATG8, autophagosome formation, and autophagic flux, concomitant with increased growth, seed set, and seed oil content (Minina et al, 2018). Overexpression of ATG8 in Arabidopsis not only increases autophagosome number and promotes autophagic activity, but also improves nitrogen remobilization efficiency and grain filling (Chen et al, 2019b).…”

Section: Autophagy During Growth and Developmentmentioning

confidence: 61%

Autophagy: An Intracellular Degradation Pathway Regulating Plant Survival and Stress Response

et al. 2020

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Autophagy is an intracellular process that facilitates the bulk degradation of cytoplasmic materials by the vacuole or lysosome in eukaryotes. This conserved process is achieved through the coordination of different autophagy-related genes (ATGs). Autophagy is essential for recycling cytoplasmic material and eliminating damaged or dysfunctional cell constituents, such as proteins, aggregates or even entire organelles. Plant autophagy is necessary for maintaining cellular homeostasis under normal conditions and is upregulated during abiotic and biotic stress to prolong cell life. In this review, we present recent advances on our understanding of the molecular mechanisms of autophagy in plants and how autophagy contributes to plant development and plants' adaptation to the environment.

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Section: Autophagy During Growth and Developmentmentioning

confidence: 61%

Autophagy: An Intracellular Degradation Pathway Regulating Plant Survival and Stress Response

et al. 2020

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“…The ATG5-ATG12 conjugate is required for autophagosome formation, closure and transfer to the vacuole [32,33]. In line with this, fewer autophagosomes were identified by MDC labelling in atg5-1 [34] and these mutants are characterized by a massive carbon starvation phenotype and early senescence. When grown on soil, they cannot survive a 5-6 day dark-induced carbon starvation ( Figure 4) [32].…”

Section: Discussionmentioning

confidence: 59%

Differential degradation of RNA species by autophagy related pathways in plants

Hickl

Drews

Girke

et al. 2019

Preprint

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An important function of the plant vacuole is the recycling of the delivered proteins and RNA by autophagy. We provide the first plant vacuolar small RNome by isolation of intact vacuoles from Barley and Arabidopsis, subsequent RNA purification and Next Generation Sequencing. In these vacuolar sRNomes, all types of cellular RNAs were found including those of chloroplast origin, suggesting a bulk-type of RNA transfer to, and breakdown in vacuoles. ATG5 is a major representative of autophagy genes and the vacuolar RNA composition in corresponding knockout plants differed clearly from controls as most chloroplast derived RNA species were missing. Moreover, the read length distribution of RNAs found in ATG5 mutants differed to control samples, indicating altered RNA processing. In contrast, vacuolar RNA length and composition of plants lacking the vacuolar RNase2 (rns2-2), involved in cellular RNA homeostasis, showed minor alterations, only. Our data therefore suggests that mainly autophagy components are responsible for selective transport and targeting of different RNA species into the vacuole for degradation. In addition, mature miRNAs were detected in all vacuolar preparations, however in ATG5 mutants at much lower frequency, indicating a new biological role for vacuolar miRNAs apart from becoming degraded.

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“…The Phvul.010G130300 gene was annotated as 'autophagy-related gene 12 (ATG12)' which is known for its roles in plant autophagic nutrient recycling during senescence and survival under N-and C-limiting conditions (Doelling et al 2002;Thompson et al 2005) through its attachment to ATG5 by a ubiquitin-like conjugation system. Plants with enhanced autophagy show elevated levels of expression of genes involved in anthocyanin and flavonoid biosynthesis (Masclaux-Daubresse et al 2014;Minina et al 2018). The genes Phvul.010G130500, Phvul.010G130600, and Phvul.010G131400 were annotated as 'MYB proteins' which are involved in transcriptional regulation of PA biosynthesis in a variety of plant species (Akagi et al 2009;Koyama et al 2014;Lu et al 2017) and were the most interesting candidates in the present study.…”

Section: Resultsmentioning

confidence: 84%

A R2R3-MYB gene-based marker for the non-darkening seed coat trait in pinto and cranberry beans (Phaseolus vulgaris L.) derived from ‘Wit-rood boontje’

Erfatpour

Pauls

2020

Theor Appl Genet

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Key message The gene Phvul.010G130600 which codes for a MYB was shown to be tightly associated with seed coat darkening in Phaseolus vulgaris and a single nucleotide deletion in the allele in Wit-rood disrupts a transcription activation region that likely prevents its functioning in this non-darkening genotype. Abstract The beige and white background colors of the seed coats of conventional pinto and cranberry beans turn brown through a process known as postharvest darkening (PHD). Seed coat PHD is attributed to proanthocyanidin accumulation and its subsequent oxidation in the seed coat. The J gene is an uncharacterized classical genetic locus known to be responsible for PHD in common bean (P. vulgaris) and individuals that are homozygous for its recessive allele have a non-darkening (ND) seed coat phenotype. A previous study identified a major colorimetrically determined QTL for seed coat color on chromosome 10 that was associated with the ND trait. The objectives of this study were to identify a gene associated with seed coat postharvest darkening in common bean and understand its function in promoting seed coat darkening. Amplicon sequencing of 21 candidate genes underlying the QTL associated with the ND trait revealed a single nucleotide deletion (c.703delG) in the candidate gene Phvul.010G130600 in non-darkening recombinant inbred lines derived from crosses between ND 'Witrood boontje' and a regular darkening pinto genotype. In silico analysis indicated that Phvul.010G130600 encodes a protein with strong amino acid sequence identity (70%) with a R2R3-MYB-type transcription factor MtPAR, which has been shown to regulate proanthocyanidin biosynthesis in Medicago truncatula seed coat tissue. The deletion in the 'Wit-rood boontje' allele of Phvul.010G130600 likely causes a translational frame shift that disrupts the function of a transcriptional activation domain contained in the C-terminus of the R2R3-MYB. A gene-based dominant marker was developed for the dominant allele of Phvul.010G130600 which can be used for marker-assisted selection of ND beans.Communicated by Albrecht E. Melchinger. Electronic supplementary materialThe online version of this article (https ://doi.org/10.1007/s0012 2-020-03571 -7) contains supplementary material, which is available to authorized users.

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Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness

Abstract: Autophagy-related proteins Atg5 and Atg7 are rate-limiting components of autophagic flux in Arabidopsis. Overexpression of ATG5 or ATG7 genes stimulates Atg8 lipidation, autophagosome formation, and autophagic flux, leading to improved plant fitness.

Cited by 134 publications

References 81 publications

Autophagy: An Intracellular Degradation Pathway Regulating Plant Survival and Stress Response

Autophagy: An Intracellular Degradation Pathway Regulating Plant Survival and Stress Response

Differential degradation of RNA species by autophagy related pathways in plants

A R2R3-MYB gene-based marker for the non-darkening seed coat trait in pinto and cranberry beans (Phaseolus vulgaris L.) derived from ‘Wit-rood boontje’

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