Transcriptional responses to phosphorus stress in the marine diatom, Chaetoceros affinis, reveal characteristic genes and expression patterns in phosphorus uptake and intracellular recycling

Shih, Chi-Yu; Kang, Lee-Kuo; Chang, Jeng Kuei

doi:10.1016/j.jembe.2015.05.001

Cited by 12 publications

(23 citation statements)

References 58 publications

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“…Concomitant with the change in relative transcript abundance, APase activity was significantly increased in the low P treatment relative to the control for G. oceanica (Table S3 ). P-dependent induction of an APase transcript was observed in C. affinis strain CCMP 160 (Shih et al, 2015 ), consistent with the observation here and parallels to G. oceanica can be inferred from transcription of APases in E. huxleyi (Xu et al, 2006 , 2010 ). It is increasingly recognized that APase activity is a common response to P stress in phytoplankton (Dyhrman, 2016 ).…”

Section: Resultssupporting

confidence: 87%

Conserved Transcriptional Responses to Nutrient Stress in Bloom-Forming Algae

et al. 2017

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The concentration and composition of bioavailable nitrogen (N) and phosphorus (P) in the upper ocean shape eukaryotic phytoplankton communities and influence their physiological responses. Phytoplankton are known to exhibit similar physiological responses to limiting N and P conditions such as decreased growth rates, chlorosis, and increased assimilation of N and P. Are these responses similar at the molecular level across multiple species? To interrogate this question, five species from biogeochemically important, bloom-forming taxa (Bacillariophyta, Dinophyta, and Haptophyta) were grown under similar low N, low P, and replete nutrient conditions to identify transcriptional patterns and associated changes in biochemical pools related to N and P stress. Metabolic profiles, revealed through the transcriptomes of these taxa, clustered together based on species rather than nutrient stressor, suggesting that the global metabolic response to nutrient stresses was largely, but not exclusively, species-specific. Nutrient stress led to few transcriptional changes in the two dinoflagellates, consistent with other research. An orthologous group analysis examined functionally conserved (i.e., similarly changed) responses to nutrient stress and therefore focused on the diatom and haptophytes. Most conserved ortholog changes were specific to a single nutrient treatment, but a small number of orthologs were similarly changed under both N and P stress in 2 or more species. Many of these orthologs were related to photosynthesis and may represent generalized stress responses. A greater number of orthologs were conserved across more than one species under low P compared to low N. Screening the conserved orthologs for functions related to N and P metabolism revealed increased relative abundance of orthologs for nitrate, nitrite, ammonium, and amino acid transporters under N stress, and increased relative abundance of orthologs related to acquisition of inorganic and organic P substrates under P stress. Although the global transcriptional responses were dominated by species-specific changes, the analysis of conserved responses revealed functional similarities in resource acquisition pathways among different phytoplankton taxa. This overlap in nutrient stress responses observed among species may be useful for tracking the physiological ecology of phytoplankton field populations.

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Section: Resultssupporting

confidence: 87%

Conserved Transcriptional Responses to Nutrient Stress in Bloom-Forming Algae

et al. 2017

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“…In both T. pseudonana and P. tricornutum this pathway is induced at the transcript level by P limitation [26,28,30]. The OPPP provides reducing power for biosynthetic processes in the form of NADPH, and may be induced to counteract lower NADPH production as a consequence of reduced photosynthesis during P limitation.…”

Section: Carbon Metabolism Responses To Phosphorus Limitation In Diatomsmentioning

confidence: 99%

“…The oxidative pentose phosphate pathway (OPPP) in diatoms appears to be cytosolic, in contrast to plants, where most steps of the pathway take place in the chloroplast [ 37 , 50 ]. In both T. pseudonana and P. tricornutum this pathway is induced at the transcript level by P limitation [ 26 , 28 , 30 ]. The OPPP provides reducing power for biosynthetic processes in the form of NADPH, and may be induced to counteract lower NADPH production as a consequence of reduced photosynthesis during P limitation.…”

Section: Carbon Metabolism Responses To Phosphorus Limitation In Diatmentioning

confidence: 99%

“…Genes encoding SQDG biosynthesis enzymes are induced under P limitation in both T. pseudonana, P. tricornutum and S. costatum , as well as in other diatoms [ 26 , 28 , 31 , 32 ]. However, P limitation does not influence expression of the SQDG biosynthesis gene SQD2 in C. affinis [ 30 ]. This may point to a higher P storage capacity in C. affinis , or a lower level of P limitation in this experiment.…”

Section: Carbon Metabolism Responses To Phosphorus Limitation In Diatmentioning

confidence: 99%

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The effects of phosphorus limitation on carbon metabolism in diatoms

Brembu¹,

Mühlroth²,

Alipanah³

et al. 2017

Phil. Trans. R. Soc. B

110

113

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Phosphorus is an essential element for life, serving as an integral component of nucleic acids, lipids and a diverse range of other metabolites. Concentrations of bioavailable phosphorus are low in many aquatic environments. Microalgae, including diatoms, apply physiological and molecular strategies such as phosphorus scavenging or recycling as well as adjusting cell growth in order to adapt to limiting phosphorus concentrations. Such strategies also involve adjustments of the carbon metabolism. Here, we review the effect of phosphorus limitation on carbon metabolism in diatoms. Two transcriptome studies are analysed in detail, supplemented by other transcriptome, proteome and metabolite data, to gain an overview of different pathways and their responses. Phosphorus, nitrogen and silicon limitation responses are compared, and similarities and differences discussed. We use the current knowledge to propose a suggestive model for the carbon flow in phosphorus-replete and phosphorus-limited diatom cells.This article is part of the themed issue ‘The peculiar carbon metabolism in diatoms’.

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“…On the other hand, P transporter proteins found in diatoms show homology with members of the SLC34 and SLC20 gene families, which are distributed in all organisms [1,[60][61][62]. The results obtained so far have demonstrated that the upregulation of genes encoding P transport proteins and APases is one of the main strategies that diatoms use to cope with P limitation [1,31,56,57,60,61].…”

Section: P Sensing In Different Organismsmentioning

confidence: 99%

Biochemical and Molecular Aspects of Phosphorus Limitation in Diatoms and Their Relationship with Biomolecule Accumulation

Lovio-Fragoso

Jesús-Campos

López‐Elías

et al. 2021

Biology

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Diatoms are the most abundant group of phytoplankton, and their success lies in their significant adaptation ability to stress conditions, such as nutrient limitation. Phosphorus (P) is a key nutrient involved in the transfer of energy and the synthesis of several cellular components. Molecular and biochemical mechanisms related to how diatoms cope with P deficiency are not clear, and research into this has been limited to a few species. Among the molecular responses that have been reported in diatoms cultured under P deficient conditions is the upregulation of genes encoding enzymes related to the transport, assimilation, remobilization and recycling of this nutrient. Regarding biochemical responses, due to the reduction of the requirements for carbon structures for the synthesis of proteins and phospholipids, more CO2 is fixed than is consumed by the Calvin cycle. To deal with this excess, diatoms redirect the carbon flow toward the synthesis of storage compounds such as triacylglycerides and carbohydrates, which are excreted as extracellular polymeric substances. This review aimed to gather all current knowledge regarding the biochemical and molecular mechanisms of diatoms related to managing P deficiency in order to provide a wider insight into and understanding of their responses, as well as the metabolic pathways affected by the limitation of this nutrient.

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Transcriptional responses to phosphorus stress in the marine diatom, Chaetoceros affinis, reveal characteristic genes and expression patterns in phosphorus uptake and intracellular recycling

Cited by 12 publications

References 58 publications

Conserved Transcriptional Responses to Nutrient Stress in Bloom-Forming Algae

Conserved Transcriptional Responses to Nutrient Stress in Bloom-Forming Algae

The effects of phosphorus limitation on carbon metabolism in diatoms

Biochemical and Molecular Aspects of Phosphorus Limitation in Diatoms and Their Relationship with Biomolecule Accumulation

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