The effects of phosphorus limitation on carbon metabolism in diatoms

Brembu, Tore; Mühlroth, Alice; Alipanah, Leila; Bones, Atle M.

doi:10.1098/rstb.2016.0406

Cited by 111 publications

(121 citation statements)

References 97 publications

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“…8). Reduction of photosynthesis and growth arrest is a common response to most stresses in diatoms suggesting that ppGpp may play an important role in stress acclimation (Litchman et al , 2003; Allen et al , 2011; Brembu et al , 2017). Other cell responses to ppGpp, such as the dramatic increase in HSP20, are similar to those found in response to dark stress (Bai et al , 2016).…”

Section: Discussionmentioning

confidence: 99%

ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

Avilán

Lebrun

Puppo

et al. 2020

Preprint

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Chloroplasts retain elements of a bacterial stress response pathway that is mediated by the signalling nucleotides guanosine penta-and tetraphosphate, or (p)ppGpp. In the model flowering plant Arabidopsis, ppGpp acts as a potent regulator of plastid gene expression and influences photosynthesis, plant growth and development.However, little is known about ppGpp metabolism or its evolution in other photosynthetic eukaryotes.• Here, we studied the function of ppGpp in the diatom P. tricornutum using transgenic lines containing an inducible system for ppGpp accumulation. We used these lines to investigate the effects of ppGpp on growth, photosynthesis, lipid metabolism and protein expression.• We demonstrate that ppGpp accumulation reduces photosynthetic capacity and promotes a quiescent-like state with reduced proliferation and ageing. Strikingly, using non-targeted proteomics, we discovered that ppGpp accumulation also leads to the coordinated upregulation of a protein protection response in multiple cellular compartments.• Our findings highlight the importance of ppGpp as a fundamental regulator of chloroplast function across different domains of life, and lead to new questions about the molecular mechanisms and roles of (p)ppGpp signalling in photosynthetic eukaryotes.

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Section: Discussionmentioning

confidence: 99%

ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

Avilán

Lebrun

Puppo

et al. 2020

Preprint

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“…In this environment, they permanently interact with other microorganisms including marine bacteria (Bruckner, Rehm, Grossart, & Kroth, ), and have to cope with fluctuations in light and nutrient availability (Magni & Montani, ; Moerdijk‐Poortvliet et al., ; Stocker, ). Therefore, microalgae have developed adaptation mechanisms and can, for example, take up and store dissolved phosphorous or nitrogen under replete conditions (Brembu, Muhlroth, Alipanah, & Bones, ; Lin, Litaker, & Sunda, ; Rogato et al., ). Some benthic diatoms including the model species Seminavis robusta are motile and move in a gliding manner by excreting extracellular polymeric substances (EPS) from elongated slits (raphae) in their biomineralized cell wall (Molino & Wetherbee, ).…”

Section: Introductionmentioning

confidence: 99%

Selective chemoattraction of the benthic diatom Seminavis robusta to phosphate but not to inorganic nitrogen sources contributes to biofilm structuring

et al. 2018

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Diatoms frequently dominate marine and freshwater biofilms as major primary producers. Nutrient resources in these biofilms are patchily distributed and fluctuate dynamically over time. We recently reported that this spatially and temporally structured environment can be exploited by motile diatoms that use chemoattraction to dissolved silicate (dSi) under Si starvation. Here, we show that the behavioral response of diatoms is more complex and selective as cells are also responding to gradients of dissolved phosphate (dP) when starved in this nutrient. In contrast, neither nitrate nor ammonium (dN) triggers an attractive response under nitrogen limitation. Video monitoring and movement pattern analysis of the model diatom Seminavis robusta revealed that dP attraction is mediated by a combined chemokinetic and chemotactic response. After locating nutrient hotspots, the microalgae slow down and recover from the limitation. The fastest recovery in terms of growth was observed after dSi limitation. In agreement with the lack of directional response, recovery from dN limitation was slowest, indicating that no short-term benefit would be drawn by the algae from the location of transient hotspots of this resource. Our results highlight the ability of diatoms to adapt to nutrient limitation by active foraging and might explain their success in patchy benthic environments.

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“…Algal growth is influenced by the transport of soluble inorganic phosphorus, which passes through the cell membrane (Singh et al 2018). Cell division is disrupted when algae suffer from the condition of P deficiency (Brembu et al 2017).…”

Section: Resultsmentioning

confidence: 99%

Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media

Susanty

Oksari

2020

Nusantara Biosci

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Abstract. Susanty D, Oksari AA. 2020. Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media. Nusantara Bioscience 12: 28-32. Intensive chicken broiler farms create waste in the environment. Chicken Broiler Waste (CBW) was used as culture media for Chlorella sp. InaCC M39 and Chlorella sorokiniana InaCC M38 at various concentrations (2, 4, 6, 8, and 10%), and compared to AF6 media. The growth of Chlorella was observed every 48 hours for 10 days by counting the number of cells using a hemocytometer. The chloroform extract of Chlorella sp. and C. sorokiniana were analyzed for their phytochemical content to determine the presence of alkaloids, flavonoids, saponin, steroids, and tannin. The results showed that 2% of CBW media was the best medium for the growth of Chlorella with nitrogen (N), phosphorus (P), and potassium (K) content was 0.01% N; 0.01% P; 0.06% K respectively. The highest specific growth rate of Chlorella sp. on 2% CBW medium was on the 8th day of culture (0.8133) with cell density of 1.6 x 106 cells/mL, while the highest specific growth rate of Chlorella sorokiniana was on the 6th day (0.8907) with cell density of 2.99 x 107 cells/mL. The chloroform extract of the biomass of Chlorella sorokiniana contained steroid and saponin

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The effects of phosphorus limitation on carbon metabolism in diatoms

Cited by 111 publications

References 97 publications

ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

Selective chemoattraction of the benthic diatom Seminavis robusta to phosphate but not to inorganic nitrogen sources contributes to biofilm structuring

Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media

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