Transcriptional responses in Japanese medaka (Oryzias latipes) exposed to binary mixtures of an estrogen and anti-estrogens

Sun, Liwei; Shao, Xiaolu; Hu, Xinhua; Chi, Jian; Jin, Yuanxiang; Ye, Weihong; Fu, Zhengwei

doi:10.1016/j.aquatox.2011.08.024

Cited by 28 publications

(16 citation statements)

References 43 publications

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“…Although one result of this type of response might be considered spurious, observation of three similar relationships by chance seems unlikely and indicates a complex effect pathway in the biological system. Similar responses were also observed in our previous study, which focused on the transcriptional profiling of genes in response to binary mixtures of estrogen and antiestrogens (Sun et al, ). In a study using the effect‐directed analysis for river sediment, Weiss et al () indicated that the methods available for toxicity screening at present were not sufficient for the evaluation of EDC cocktails, which are generally encountered in the “real world.” The results obtained in this study provided further evidence for the argument that the effects of mixtures are not well predicted by gene transcription profiles based on the results of individual chemicals, as we suggested in the our previous report (Sun et al, ).…”

Section: Discussionsupporting

confidence: 88%

“…Similar responses were also observed in our previous study, which focused on the transcriptional profiling of genes in response to binary mixtures of estrogen and antiestrogens (Sun et al, ). In a study using the effect‐directed analysis for river sediment, Weiss et al () indicated that the methods available for toxicity screening at present were not sufficient for the evaluation of EDC cocktails, which are generally encountered in the “real world.” The results obtained in this study provided further evidence for the argument that the effects of mixtures are not well predicted by gene transcription profiles based on the results of individual chemicals, as we suggested in the our previous report (Sun et al, ). Therefore, the identification of chemicals that cause effects induced by exposure to environmental samples, which are much more complicated than the binary mixtures tested in most studies, remains a daunting challenge in ecotoxicology.…”

Section: Discussionsupporting

confidence: 88%

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Transcriptional responses in male Japanese medaka exposed to antiandrogens and antiandrogen/androgen mixtures

Sun

Peng

Liu

et al. 2015

Environmental Toxicology

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The occurrence of androgenic endocrine disrupting chemicals (EDCs) in water is thought to be linked to deviation from normal male developmental and reproductive functions in exposed aquatic organisms. Because aquatic environments represent a chemically complex medium, the combined effects of androgenic EDCs require urgent attention. In the present study, the effects of two model androgen receptor (AR) antagonists, flutamide (FLU), and vinclozolin (VIN), were first determined individually in male Japanese medaka using the transcriptional response for genes associated with the hypothalamic-pituitary-gonadal axis. The fish were further exposed to binary mixtures of VIN and 17β-trenbolone (TRE, AR agonist) to confirm the theoretical opposing effects of the AR antagonist and agonist. The results showed that exposure to FLU or VIN alone induced very similar transcriptional responses, demonstrating that gene transcription analysis could be successfully employed in identifying the action of single chemicals. For example, both exposures increased the transcription of cyp17b but decreased that of cyp19b in the gonad, demonstrating the compensatory response for AR blockage. However, in the case of exposure to mixtures, although the joint antagonistic action of TRE and VIN affected the most genes, the transcription profiles after exposure to mixtures were not consistent with expectations based on the results for individual chemicals, such as hepatic vtg, and star or cyp19a in gonads. Therefore, the limitation of gene transcription analyses in exposures to mixtures, as well as the potential for the extrapolation of single chemicals, should be considered in future studies. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1591-1599, 2016.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 88%

Transcriptional responses in male Japanese medaka exposed to antiandrogens and antiandrogen/androgen mixtures

Sun

Peng

Liu

et al. 2015

Environmental Toxicology

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“…At higher doses, interactions either synergistic (supra-additive) or antagonistic (infra-additive) are more easy to identify. Antagonistic effects are reported in response to co-exposure to EDCs with known MoA, namely estrogens and antiestrogens (Sun et al, 2011;Wu et al, 2014). Toxicokinetic interactions occur when a contaminant modifies the absorption of others (e.g., Tan et al, 2012;Su et al, 2013).…”

Section: Emerging Concern With Legacy Pollutants and Emerging Contamimentioning

confidence: 99%

Introduction

Amiard‐Triquet¹

2015

Aquatic Ecotoxicology

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The aquatic environment appears as the final destination for most of anthropogenic contaminants released from industry, agriculture, urbanization, transport, tourism, and everyday life. On the other hand, inland, coastal, and marine waters provide many services important for human well-being. The conservation of ecosystems and human health is based on a sound assessment of the risks associated with the presence of contaminants in the aquatic environment. The aim of this book is to use cross-analyses of procedures, biological models, and contaminants to design ecotoxicological tools suitable for better environmental assessments, particularly in the case of emerging contaminants and emerging concern with legacy pollutants.

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“…There is a growing concern about a high variety of environmental pollutants affecting human and wildlife health (Sun et al, 2011). Among them, there is a family of contaminants known generically as endocrine disrupting chemicals (EDCs).…”

Section: Introductionmentioning

confidence: 99%

Vitellogenin expression in wild cyprinid Petroleuciscus esfahani as a biomarker of endocrine disruption along the Zayandeh Roud River, Iran

et al. 2016

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Aquatic environments are the ultimate sink for most of anthropogenic pollutants. The Zayandeh Roud River is the most important river in the central Iranian Plateau, supplying water to a large population. In order to determine the potential occurrence and in vivo effects of Endocrine Disrupting Chemicals (EDCs) with estrogenic or anti-androgenic properties we analyzed the wild populations of an extensively distributed endemic fish species, Petroleuciscus esfahani. For this purpose, specimens were caught from two sites upstream and two sites downstream of the expected major anthropogenic pollution sources. P. esfahani full-length cDNAs for vitellogenin (vtg), with 4177 base pairs (bp) encoding a 1339 amino acids (aa), and for β-actin (actb), with 1776 bp encoding a 375 aa, were amplified and cloned. Hepatic vtg mRNA expression levels were measured by quantitative real-time PCR. Condition factor, gonadosomatic index and sex ratio were calculated and compared with vtg expression. Gonad histology was performed to study the possible presence of intersex condition. Detection of vtg transcripts in male individuals from the two downstream sampling sites supports the hypothesis of exposure to EDCs in these regions. Higher vtg expression in male individuals, together with reduced gonad size and condition factor, in specimens from the site located downstream of the major steel mill plant suggest a major endocrine disruption in this area.

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Transcriptional responses in Japanese medaka (Oryzias latipes) exposed to binary mixtures of an estrogen and anti-estrogens

Cited by 28 publications

References 43 publications

Transcriptional responses in male Japanese medaka exposed to antiandrogens and antiandrogen/androgen mixtures

Transcriptional responses in male Japanese medaka exposed to antiandrogens and antiandrogen/androgen mixtures

Introduction

Vitellogenin expression in wild cyprinid Petroleuciscus esfahani as a biomarker of endocrine disruption along the Zayandeh Roud River, Iran

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