Transcriptional response of the terpenoid indole alkaloid pathway to the overexpression of ORCA3 along with jasmonic acid elicitation of Catharanthus roseus hairy roots over time

Peebles, Christie A. M.; Hughes, Erik H.; Shanks, Jacqueline V.; San, Ka‐Yiu

doi:10.1016/j.ymben.2008.09.002

Cited by 135 publications

(114 citation statements)

References 35 publications

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“…Alternatively, CrWRKY1 regulates ORCA3 by repressing CrMYC2, which controls the JA-responsive expression of ORCA3 (Zhang et al, 2011). Our results are in agreement with recent observations showing that JA induction increases TIA accumulation in C. roseus hairy roots, yet, overexpression of ORCA3 does not enhance TIA production (Peebles et al, 2009). Correspondingly, JA elicitation, but not ORCA3 overexpression, up-regulates TDC expression.…”

Section: Overexpression or Repression Of Crwrky1 Affects The Accumulasupporting

confidence: 92%

“…In general, the transcript levels of TDC, DXS, G10H, SGD, and ORCA2 are lower in the ORCA3-overexpressing lines than in the JA-induced lines. The authors thus concluded that (1) a negative regulatory mechanism overrides the activation of these genes by ORCA3 and (2) another strong positive regulator of the TIA pathway exists in C. roseus (Peebles et al, 2009). Here, we demonstrate that CrWRKY1 is a negative regulator of ORCA3 and a positive regulator of the TIA pathway.…”

Section: Overexpression or Repression Of Crwrky1 Affects The Accumulasupporting

confidence: 60%

“…Relying on the detoxification of 4-MT as selection has led to the isolation of ORCA3, an AP2-like TF that upregulates TDC (van der . Overexpression of ORCA3 increases TDC activity and the accumulation of tryptamine in C. roseus suspension cells (van der Fits and Memelink, 2000) but not in hairy roots (Peebles et al, 2009). In contrast, CrWRKY1 hairy roots not only have increased TDC activity but also accumulate nearly 2-fold more tryptamine than control roots.…”

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confidence: 99%

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The Transcription Factor CrWRKY1 Positively Regulates the Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus

et al. 2011

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Catharanthus roseus produces a large array of terpenoid indole alkaloids (TIAs) that are an important source of natural or semisynthetic anticancer drugs. The biosynthesis of TIAs is tissue specific and induced by certain phytohormones and fungal elicitors, indicating the involvement of a complex transcriptional control network. However, the transcriptional regulation of the TIA pathway is poorly understood. Here, we describe a C. roseus WRKY transcription factor, CrWRKY1, that is preferentially expressed in roots and induced by the phytohormones jasmonate, gibberellic acid, and ethylene. The overexpression of CrWRKY1 in C. roseus hairy roots up-regulated several key TIA pathway genes, especially Tryptophan Decarboxylase (TDC), as well as the transcriptional repressors ZCT1 (for zinc-finger C. roseus transcription factor 1), ZCT2, and ZCT3. However, CrWRKY1 overexpression repressed the transcriptional activators ORCA2, ORCA3, and CrMYC2. Overexpression of a dominant-repressive form of CrWRKY1, created by fusing the SRDX repressor domain to CrWRKY1, resulted in the downregulation of TDC and ZCTs but the up-regulation of ORCA3 and CrMYC2. CrWRKY1 bound to the W box elements of the TDC promoter in electrophoretic mobility shift, yeast one-hybrid, and C. roseus protoplast assays. Up-regulation of TDC increased TDC activity, tryptamine concentration, and resistance to 4-methyl tryptophan inhibition of CrWRKY1 hairy roots. Compared with control roots, CrWRKY1 hairy roots accumulated up to 3-fold higher levels of serpentine. The preferential expression of CrWRKY1 in roots and its interaction with transcription factors including ORCA3, CrMYC2, and ZCTs may play a key role in determining the root-specific accumulation of serpentine in C. roseus plants.

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Section: Overexpression or Repression Of Crwrky1 Affects The Accumulasupporting

confidence: 92%

Section: Overexpression or Repression Of Crwrky1 Affects The Accumulasupporting

confidence: 60%

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confidence: 99%

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The Transcription Factor CrWRKY1 Positively Regulates the Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus

et al. 2011

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“…4B). Previously, MIA metabolite levels had not been found to increase significantly in ORCA3-overexpressing C. roseus hairy root lines either, not even after loganin and tryptophan feeding (23). This observation correlated not only with the anticipated lack of G8O induction but, unexpectedly, also with unaltered tryptophan decarboxylase (TDC) and repressed strictosidine-β-glucosidase (SGD) expression (23), two ORCA3-inducible genes in cell cultures (14).…”

Section: Bis1 Specifically Up-regulates the Iridoid Genes In C Roseumentioning

confidence: 94%

“…The use of TFs can be a powerful tool in such metabolic engineering programs. ORCA3 has been overexpressed in cell suspensions, hairy roots, and plants in attempts to increase MIA production (14,(23)(24)(25). However, because ORCA3 does not activate iridoid genes, MIA accumulation in these lines required either coexpression with G8O or feeding with iridoid precursors.…”

Section: Significancementioning

confidence: 99%

The bHLH transcription factor BIS1 controls the iridoid branch of the monoterpenoid indole alkaloid pathway in Catharanthus roseus

Moerkercke

Steensma

Schweizer

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

172

163

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Plants make specialized bioactive metabolites to defend themselves against attackers. The conserved control mechanisms are based on transcriptional activation of the respective plant speciesspecific biosynthetic pathways by the phytohormone jasmonate. Knowledge of the transcription factors involved, particularly in terpenoid biosynthesis, remains fragmentary. By transcriptome analysis and functional screens in the medicinal plant Catharanthus roseus (Madagascar periwinkle), the unique source of the monoterpenoid indole alkaloid (MIA)-type anticancer drugs vincristine and vinblastine, we identified a jasmonate-regulated basic helix-loop-helix (bHLH) transcription factor from clade IVa inducing the monoterpenoid branch of the MIA pathway. The bHLH iridoid synthesis 1 (BIS1) transcription factor transactivated the expression of all of the genes encoding the enzymes that catalyze the sequential conversion of the ubiquitous terpenoid precursor geranyl diphosphate to the iridoid loganic acid. BIS1 acted in a complementary manner to the previously characterized ethylene response factor Octadecanoid derivative-Responsive Catharanthus APETALA2-domain 3 (ORCA3) that transactivates the expression of several genes encoding the enzymes catalyzing the conversion of loganic acid to the downstream MIAs. In contrast to ORCA3, overexpression of BIS1 was sufficient to boost production of highvalue iridoids and MIAs in C. roseus suspension cell cultures. Hence, BIS1 might be a metabolic engineering tool to produce sustainably high-value MIAs in C. roseus plants or cultures.basic helix loop helix | Catharanthus roseus | jasmonate | Madagascar periwinkle | iridoids

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Regulatory and Biosynthetic Mechanisms Underlying Plant Chemical Defense Responses to Biotic Stresses

Chezem

Clay

2015

Molecular Mechanisms in Plant Adaptation

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Transcriptional response of the terpenoid indole alkaloid pathway to the overexpression of ORCA3 along with jasmonic acid elicitation of Catharanthus roseus hairy roots over time

Cited by 135 publications

References 35 publications

The Transcription Factor CrWRKY1 Positively Regulates the Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus

The Transcription Factor CrWRKY1 Positively Regulates the Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus

The bHLH transcription factor BIS1 controls the iridoid branch of the monoterpenoid indole alkaloid pathway in Catharanthus roseus

Regulatory and Biosynthetic Mechanisms Underlying Plant Chemical Defense Responses to Biotic Stresses

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