Regulatory and Biosynthetic Mechanisms Underlying Plant Chemical Defense Responses to Biotic Stresses

Chezem, William R.; Clay, Nicole K.

doi:10.1002/9781118860526.ch5

Cited by 1 publication

(1 citation statement)

References 165 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these pathways, the flavonoid biosynthesis pathway is a ‘model’ for secondary metabolite production. Flavonoids perform different functions in plants, including color formation and resistance to abiotic and biotic stresses ( Nishihara and Nakatsuka, 2011 ; Chezem and Clay, 2015 ; Davies et al., 2018 ). Much effort has focused on revealing the flavonoid biosynthesis pathway involved in seed color formation using Arabidopsis TRANSPARENT TESTA ( TT ) mutants, such as mutants of TT1 - TT19 , TTG1 , and TTG2 ( Albert et al., 1997 ; Dong et al., 2001 ; Bharti and Khurana, 2003 ; Kerhoas et al., 2006 ; Fu et al., 2007 ; Stracke et al., 2007 ; Yoshida et al., 2008 ; Cutanda-Perez et al., 2009 ; Baudry et al., 2010 ; Appelhagen et al., 2011 ; Chen et al., 2013 ; Pang et al., 2013 ; Stein et al., 2013 ; Zhang et al., 2013 ; Gesell et al., 2014 ; Ichino et al., 2015 ; Gonzalez et al., 2016 ; Zhang et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis identifies candidate genes related to seed coat color in rapeseed

Guan

Shi²,

Chen³

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Yellow seed coat in rapeseed (Brassica napus) is a desirable trait that can be targeted to improve the quality of this oilseed crop. To better understand the inheritance mechanism of the yellow-seeded trait, we performed transcriptome profiling of developing seeds in yellow- and black-seeded rapeseed with different backgrounds. The differentially expressed genes (DEGs) during seed development showed significant characteristics, these genes were mainly enriched for the Gene Ontology (GO) terms carbohydrate metabolic process, lipid metabolic process, photosynthesis, and embryo development. Moreover, 1206 and 276 DEGs, which represent candidates to be involved in seed coat color, were identified between yellow- and black-seeded rapeseed during the middle and late stages of seed development, respectively. Based on gene annotation, GO enrichment analysis, and protein–protein interaction network analysis, the downregulated DEGs were primarily enriched for the phenylpropanoid and flavonoid biosynthesis pathways. Notably, 25 transcription factors (TFs) involved in regulating flavonoid biosynthesis pathway, including known (e.g., KNAT7, NAC2, TTG2 and STK) and predicted TFs (e.g., C2H2-like, bZIP44, SHP1, and GBF6), were identified using integrated gene regulatory network (iGRN) and weight gene co-expression networks analysis (WGCNA). These candidate TF genes had differential expression profiles between yellow- and black-seeded rapeseed, suggesting they might function in seed color formation by regulating genes in the flavonoid biosynthesis pathway. Thus, our results provide in-depth insights that facilitate the exploration of candidate gene function in seed development. In addition, our data lay the foundation for revealing the roles of genes involved in the yellow-seeded trait in rapeseed.

show abstract