Transcriptional regulation of the waaAE-coaD operon by PhoP and RcsAB in Yersinia pestis biovar Microtus

Liu, Lei; Fang, Nan; Sun, Yicheng; Yang, Huiying; Zhang, Yiquan; Han, Yanping; Zhou, Dongsheng

doi:10.1007/s13238-014-0110-8

Cited by 11 publications

(11 citation statements)

References 10 publications

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“…Therefore, CRP- and PhoP-mediated regulation of Y. pestis biofilm production may be dependent on a similar mechanism. According to the conclusions obtained in this study and previous studies ( Rebeil et al, 2013 ; Zhang et al, 2013a , 2015 ; Liu et al, 2014 ), the complex regulatory interactions of cAMP-CRP and PhoP/PhoQ facilitate the cellular pathways governed by these two regulatory systems to merge into a single global regulatory circuit in Y. pestis , which responds to environmental stimuli and controls the expression of genes involved in various functions in flea vectors, thereby enabling better survival and transmission of this pathogen.…”

Section: Discussionsupporting

confidence: 71%

“…Therefore, CRP-dependent activation of phoPQ -YPO1632 occurs in a direct manner, while CRP-dependent activation of waaAE-coaD occurs in an indirect manner. In addition, our previous study showed that PhoP directly stimulates the transcription of waaAE-coaD ( Liu et al, 2014 ), and thereby CRP-mediated activation of waaAE-coaD is most likely to function by directly acting on phoPQ -YPO1632.…”

Section: Resultsmentioning

confidence: 99%

“…The DNase I footprinting experiments were carried out to determine the precise CRP sites for the direct CRP target genes. Collection of data from the translational/transcriptional start sites, the core promoter –10 and –35 elements, the Shine–Dalgarno sequences for ribosomal binding, and the CRP sites (in this study) and PhoP sites ( Zhang et al, 2013a ; Liu et al, 2014 ) enabled us to characterize the organization of the promoters of gmhA ( Figure 5A ), phoP ( Figure 5B ), and waaA ( Figure 5C ).…”

Section: Resultsmentioning

confidence: 99%

“…However, CRP is able to stimulate biofilm development by directly activating the transcription of gmhA and meanwhile indirectly promoting the transcription of waaAE-coaD. CRP stimulates the transcription of waaAE-coaD through acting on PhoPQ , a direct activator of waaAE-coaD ( Liu et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, PhoPQ is induced by low pH in the flea gut, and controls physiological adaptation to acid and other stresses encountered during infection of the fleas; it simultaneously stimulates the formation of flea-borne infectious Y. pestis biofilms ( Rebeil et al, 2013 ; Vadyvaloo et al, 2015 ). Nevertheless, PhoP-mediated regulation of Y. pestis biofilm formation relies on its modulation of the waaAE-coaD operon rather than the hms genes ( Liu et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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CRP Is an Activator of Yersinia pestis Biofilm Formation that Operates via a Mechanism Involving gmhA and waaAE-coaD

et al. 2016

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gmhA encodes a phosphoheptose isomerase that catalyzes the biosynthesis of heptose, a conserved component of lipopolysaccharide (LPS). GmhA plays an important role in Yersinia pestis biofilm blockage in the flea gut. waaA, waaE, and coaD constitute a three-gene operon waaAE-coaD in Y. pestis. waaA encodes a transferase that is responsible for binding lipid-A to the core oligosaccharide of LPS. WaaA is a key determinant in Y. pestis biofilm formation, and the waaA expression is positively regulated by the two-component regulatory system PhoP/PhoQ. WaaE is involved in LPS modification and is necessary for Y. pestis biofilm production. In this study, the biofilm-related phenotypic assays indicate that the global regulator CRP stimulates Y. pestis biofilm formation in vitro and on nematodes, while it has no regulatory effect on the biosynthesis of the biofilm-signaling molecular 3′,5′-cyclic diguanosine monophosphate. Further gene regulation experiments disclose that CRP does not regulate the hms genes at the transcriptional level but directly promotes the gmhA transcription and indirectly activates the waaAE-coaD transcription through directly acting on phoPQ-YPO1632. Thus, it is speculated that CRP-mediated carbon catabolite regulation of Y. pestis biofilm formation depends on the CRP-dependent carbon source metabolic pathways of the biosynthesis, modification, and transportation of biofilm exopolysaccharide.

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Section: Discussionsupporting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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CRP Is an Activator of Yersinia pestis Biofilm Formation that Operates via a Mechanism Involving gmhA and waaAE-coaD

et al. 2016

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Genetic Regulation of Yersinia pestis

Han

Fang

Liu

et al. 2016

Advances in Experimental Medicine and Biology

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Y. pestis exhibits dramatically different traits of pathogenicity and transmission, albeit their close genetic relationship with its ancestor-Y. pseudotuberculosis, a self-limiting gastroenteric pathogen. Y. pestis is evolved into a deadly pathogen and transmitted to mammals and/or human beings by infected flea biting or directly contacting with the infected animals. Various kinds of environmental changes are implicated into its complex life cycle and pathogenesis. Dynamic regulation of gene expression is critical for environmental adaptation or survival, primarily reflected by genetic regulation mediated by transcriptional factors and small regulatory RNAs at the transcriptional and posttranscriptional level, respectively. The effects of genetic regulation have been shown to profoundly influence Y. pestis physiology and pathogenesis such as stress resistance, biofilm formation, intracellular survival, and replication. In this chapter, we mainly summarize the progresses on popular methods of genetic regulation and on regulatory patterns and consequences of many key transcriptional and posttranscriptional regulators, with a particular emphasis on how genetic regulation influences the biofilm and virulence of Y. pestis.

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Transcriptional Regulation Between the Two Global Regulators RovA and CRP in Yersinia pestis biovar Microtus

et al. 2018

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Transcriptional regulation of the waaAE-coaD operon by PhoP and RcsAB in Yersinia pestis biovar Microtus

Cited by 11 publications

References 10 publications

CRP Is an Activator of Yersinia pestis Biofilm Formation that Operates via a Mechanism Involving gmhA and waaAE-coaD

CRP Is an Activator of Yersinia pestis Biofilm Formation that Operates via a Mechanism Involving gmhA and waaAE-coaD

Genetic Regulation of Yersinia pestis

Transcriptional Regulation Between the Two Global Regulators RovA and CRP in Yersinia pestis biovar Microtus

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