Transcriptional regulation of the flavonoid pathway in the skin of dark-grown ‘Cripps’ Red’ apples in response to sunlight

Takos, M. Adam; Robinson, P.P.; Walker, Amanda R.

doi:10.1080/14620316.2006.11512131

Cited by 47 publications

(34 citation statements)

References 27 publications

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“…5A). This is similar to the pattern observed for MdUFGT transcript levels and correlates with anthocyanin synthesis when bagged fruit were uncovered (Takos et al, 2006a). In control fruit, which were grown under normal light conditions, MdMYB1 transcripts were approximately 25-fold higher than in the bagged fruit and this level did not fluctuate by more than approximately 2-fold throughout the course of the experiment.…”

Section: Mdmyb1 Expression Analysissupporting

confidence: 63%

“…Walker, and A.M. Takos, personal communication). Coinciding with the reexposure to sunlight, we found increased transcript levels of all the flavonoid structural genes required for anthocyanin synthesis: MdCHS, MdCHI, MdF3H, MdDFR, MdLDOX, and MdUFGT, but synthesis of flavonols and CTs was not induced (Takos et al, 2006a).…”

Section: Isolation Of Mdmyb1mentioning

confidence: 84%

“…Consistent with a role in regulating anthocyanin synthesis, MdMYB1 gene transcripts increased within 1 d when bags were removed from apple fruit exposing skin to sunlight. This increase in MdMYB1 transcripts was accompanied by the accumulation of anthocyanin and flavonoid structural gene transcripts in the skin (Takos et al, 2006a). Other anthocyanin MYB regulators have also been shown to respond to light; for example, in Arabidopsis anthocyanin synthesis and expression of both AtPAP1 and AtPAP2 respond to light under stress conditions (Vanderauwera et al, 2005).…”

Section: Characterization Of Mdmyb1mentioning

confidence: 99%

“…The anthocyanin pathway was manipulated artificially in apples by covering fruit on trees with bags early in the season to exclude sunlight. When the bags were removed during the fruit ripening phase, reexposure to sunlight induced anthocyanin synthesis after about 2 to 3 d in Cripps' Red and Cripps' Pink fruit (Takos et al, 2006a; J.-L. Parker, S.P. Robinson, A.R.…”

Section: Isolation Of Mdmyb1mentioning

confidence: 99%

“…We recently demonstrated that in the red skin cultivar Cripps' Red, several flavonoid genes required for anthocyanin synthesis were coordinately transcribed in response to light exposure (Takos et al, 2006a). However, the expression of the MdLAR1 and MdANR genes, required for synthesis of CTs, was not light regulated.…”

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confidence: 99%

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Light-Induced Expression of a MYB Gene Regulates Anthocyanin Biosynthesis in Red Apples

Takos¹,

Jaffé²,

Jacob³

et al. 2006

Plant Physiol.

888

776

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Anthocyanins are secondary metabolites found in higher plants that contribute to the colors of flowers and fruits. In apples (Malus domestica Borkh.), several steps of the anthocyanin pathway are coordinately regulated, suggesting control by common transcription factors. A gene encoding an R2R3 MYB transcription factor was isolated from apple (cv Cripps' Pink) and designated MdMYB1. Analysis of the deduced amino acid sequence suggests that this gene encodes an ortholog of anthocyanin regulators in other plants. The expression of MdMYB1 in both Arabidopsis (Arabidopsis thaliana) plants and cultured grape cells induced the ectopic synthesis of anthocyanin. In the grape (Vitis vinifera) cells MdMYB1 stimulated transcription from the promoters of two apple genes encoding anthocyanin biosynthetic enzymes. In ripening apple fruit the transcription of MdMYB1 was correlated with anthocyanin synthesis in red skin sectors of fruit. When dark-grown fruit were exposed to sunlight, MdMYB1 transcript levels increased over several days, correlating with anthocyanin synthesis in the skin. MdMYB1 gene transcripts were more abundant in red skin apple cultivars compared to non-red skin cultivars. Several polymorphisms were identified in the promoter of MdMYB1. A derived cleaved amplified polymorphic sequence marker designed to one of these polymorphisms segregated with the inheritance of skin color in progeny from a cross of an unnamed red skin selection (a sibling of Cripps' Pink) and the non-red skin cultivar Golden Delicious. We conclude that MdMYB1 coordinately regulates genes in the anthocyanin pathway and the expression level of this regulator is the genetic basis for apple skin color.

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Section: Mdmyb1 Expression Analysissupporting

confidence: 63%

Section: Isolation Of Mdmyb1mentioning

confidence: 84%

Section: Characterization Of Mdmyb1mentioning

confidence: 99%

Section: Isolation Of Mdmyb1mentioning

confidence: 99%

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confidence: 99%

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