Transcriptional regulation of PaPYLs, PaPP2Cs and PaSnRK2s during sweet cherry fruit development and in response to abscisic acid and auxin at onset of fruit ripening

Abstract

To further understand the basis of abscisic acid (ABA) signal transduction in regulating sweet cherry fruit development and ripening, and also the interaction between ABA and indole acetic acid (IAA) at the onset of fruit ripening, full-length or partial cDNA of three PaPYLs (ABA receptor), six PaPP2Cs (type 2C protein phosphatase) and six PaSnRK2s (subfamily 2 of SNF1-related kinases) were identified from sweet cherry. Multiple alignments and phylogeny analyses showed that most of the functional residues or domains were well conserved within each gene family; all PaPP2Cs belonged to the group A PP2C; PaSnRK2.4 and PaSnRK2.5 belonged to subclass III of SnRK2. PaPYL2/3, PaPP2C3/4/6 and PaSnRK2.4 were highly expressed at the early stages of fruit development. Moreover, PaPYL2, PaPP2C3/4, PaSnRK2.4 also had a high expression level at the onset of fruit ripening. Exogenous ABA treatment at 28 days after full bloom decreased the IAA level and promoted fruit ripening by increasing anthocyanin and soluble solids content. However, although IAA treatment induced the ABA accumulation, it delayed the onset of fruit ripening. Most of the PaPYLs and PaSnRK2s didn't strongly respond to ABA and IAA treatments at 28 days after full bloom, while expressions of PaPP2C3, PaPP2C5 and PaPP2C6 were significantly induced by exogenous ABA and PaPYL1 was significantly induced by exogenous IAA.

PacCYP707A2 negatively regulates cherry fruit ripening while PacCYP707A1 mediates drought tolerance

“…4 ). Three ABA receptor genes ( PYLs ), six 2C protein phosphatases ( PP2Cs ), and six subfamily 2 SNF1-related kinases ( SnRK2 s) were previously cloned from cherry fruits ( Wang et al , 2015 ). Of these, PacPYL2, PacPP2C3 , and PacSnRK2.3 were strongly expressed in sweet cherry fruits during ripening, but expression of the other genes was very low.…”

A novel sucrose transporter gene IbSUT4 involves in plant growth and response to abiotic stress through the ABF-dependent ABA signaling pathway in Sweetpotato

“…ABA is a broad-spectrum phytohormone involved in the regulation of stomatal opening, growth and development, and coordination of various stress signal transduction pathways during abiotic stress [16,17]. To date, several ABA-pathway genes responsible for plant tolerance to abiotic stress have been identified, including the ABA receptor PYL8/RCAR3 and the regulatory factors SNF1 (SNF1-activated protein kinase) and PP2C (2C protein phosphatases) [18][19][20][21]. Moreover, it has been reported that ABA is associated with the sugar response pathway in plants [22].…”

Implication of Abscisic Acid on Ripening and Quality in Sweet Cherries: Differential Effects during Pre- and Post-harvest

“…It has been shown that high concentrations of abscisic acid (ABA) are required for ripening in sweet cherries ( Luo et al, 2013 ; Wang et al, 2015 ). ABA is a sesquiterpenoid hormone, derived from carotenoids, that is implicated in several physiological processes, from seed dormancy to senescence processes, including plant stress responses and the regulation of fruit development ( Nambara and Marion-Poll, 2005 ; Finkelstein, 2013 ; Leng et al, 2014 ).…”