PacCYP707A2 negatively regulates cherry fruit ripening while PacCYP707A1 mediates drought tolerance

Li, Qian; Chen, Pei; Dai, Shengjie; Sun, Yufei; Yuan, Bing; Kai, Wenbin; Pei, Yuelin; He, S.; Liang, Bin; Zhang, Yushu; Leng, Ping

doi:10.1093/jxb/erv169

Cited by 59 publications

(49 citation statements)

References 42 publications

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“…Moreover, down-regulation of FaNCED1 , which encodes 9- cis -epoxycarotenoid dioxygenase (a rate-limiting enzyme in ABA biosynthesis), and FaBG3 , which encodes β-glucosidase (an enzyme which hydrolyzes ABA glucose esters to release free ABA), led to the delay of fruit ripening and coloration, presumably due to a decrease of free ABA levels in these fruits [12, 14]. These data are supported by additional work performed in sweet cherry, where decreasing the transcriptional level of PacCYP707A2 , which encodes a key enzyme in the oxidative catabolism of ABA, via VIGS (Virus Induced Gene Silencing), led to an increase in the ABA content and promoted the coloration and ripening of fruit [15]. Recent work has demonstrated that ABA is not only important in the ripening of non-climacteric fruits, but also plays an essential role in the ripening of several climacteric fruits, particularly tomato.…”

Section: Introductionmentioning

confidence: 73%

Abscisic acid pathway involved in the regulation of watermelon fruit ripening and quality trait evolution

et al. 2017

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Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) is a non-climacteric fruit. The modern sweet-dessert watermelon is the result of years of cultivation and selection for fruits with desirable qualities. To date, the mechanisms of watermelon fruit ripening, and the role of abscisic acid (ABA) in this process, has not been well understood. We quantified levels of free and conjugated ABA contents in the fruits of cultivated watermelon (97103; C. lanatus subsp. vulgaris), semi-wild germplasm (PI179878; C. lanatus subsp. mucosospermus), and wild germplasm (PI296341-FR; C. lanatus subsp. lanatus). Results showed that ABA content in the fruits of 97103 and PI179878 increased during fruit development and ripening, but maintained a low steady state in the center flesh of PI296341-FR fruits. ABA levels in fruits were highest in 97103 and lowest in PI296341-FR, but no obvious differences in ABA levels were observed in seeds of these lines. Examination of 31 representative watermelon accessions, including different C. lanatus subspecies and ancestral species, showed a correlation between soluble solids content (SSC) and ABA levels in ripening fruits. Furthermore, injection of exogenous ABA or nordihydroguaiaretic acid (NDGA) into 97103 fruits promoted or inhibited ripening, respectively. Transcriptomic analyses showed that the expression levels of several genes involved in ABA metabolism and signaling, including Cla009779 (NCED), Cla005404 (NCED), Cla020673 (CYP707A), Cla006655 (UGT) and Cla020180 (SnRK2), varied significantly in cultivated and wild watermelon center flesh. Three SNPs (-738, C/A; -1681, C/T; -1832, G/T) in the promoter region of Cla020673 (CYP707A) and one single SNP (-701, G/A) in the promoter of Cla020180 (SnRK2) exhibited a high level of correlation with SSC variation in the 100 tested accessions. Our results not only demonstrate for the first time that ABA is involved in the regulation of watermelon fruit ripening, but also provide insights into the evolutionary mechanisms of this phenomenon.

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Section: Introductionmentioning

confidence: 73%

Abscisic acid pathway involved in the regulation of watermelon fruit ripening and quality trait evolution

et al. 2017

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“…The expression of AAO3 and ABA2 were elevated in dehydration stressed Arabidopsis, which explained that AAO3 and ABA2 participated in plants tolerance to drought stress [45][46][47] . While abscisic acid 8'-hydroxylases (CYP707A1) involved in ABA catabolism, regulated ABA content in Arabidopsis, sweet cherry 48,49 . Besides, ABA biosynthesis in root was also regulated by leaf dehydration to trigger ABA induced responses in roots 37 .…”

Section: Discussionmentioning

confidence: 99%

Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis

Yang

Guo

Zhong

et al. 2020

Sci Rep

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Opisthopappus taihangensis (Ling) Shih, as a relative of chrysanthemum, mainly survives on the cracks of steep slopes and cliffs. Due to the harsh environment in which O. taihangensis lives, it has evolved strong adaptive traits to drought stress. The root system first perceives soil water deficiency, triggering a multi-pronged response mechanism to maintain water potential; however, the drought tolerance mechanism of O. taihangensis roots remains unclear. Therefore, roots were selected as materials to explore the physiological and molecular responsive mechanisms. We found that the roots had a stronger water retention capacity than the leaves. This result was attributed to ABA accumulation, which promoted an increased accumulation of proline and trehalose to maintain cell osmotic pressure, activated SOD and POD to scavenge ROS to protect root cell membrane structure and induced suberin depositions to minimize water backflow to dry soil. Transcriptome sequencing analyses further confirmed that O. taihangensis strongly activated genes involved in the ABA signalling pathway, osmolyte metabolism, antioxidant enzyme activity and biosynthesis of suberin monomer. overall, these results not only will provide new insights into the drought response mechanisms of O. taihangensis but also will be helpful for future drought breeding programmes of chrysanthemum. Drought stress is one of the most common abiotic stresses that threatens the healthy growth and development of plants. With the further aggravation of global warming and shortages of fresh water associated with population growth, it is estimated that drought stress will severely reduce the yield and quality of crops and ornamental plants 1. Therefore, further exploration of the physiological and molecular mechanisms is necessary for breeding drought-tolerant plants. Plants have evolved physiological, biochemical and molecular strategies to adapt to arid environments and to prevent cells from water deficiency. Physiological adaptability, including abscisic acid (ABA) content changes, proline accumulation, and superoxide dismutase (SOD) and peroxidase (POD) enzyme activities, are fundamental for plants to withstand drought stress 2-5. Drought-responsive molecular mechanisms have been divided into two terms: those that directly protect plants against drought stress and those that regulate targeted gene expression and signal transduction in plants in response to drought 6. The first term includes genes encoding proteins that function by protecting cell turgor, such as enzymes participating in the biosynthesis of various osmoprotectants 4,7,8. Moreover, late-embryogenesis-abundant proteins, chaperones and antioxidant enzymes directly prevent plants from drought damage. The second term of genes mainly comprises transcription factors, which are activated by signal transduction pathways and regulate functional genes 2,9. Furthermore, protein kinases, protein phosphatases, enzymes related to phospholipid metabolism and ubiquitin ligase play significant roles in the signal trans...

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“…Another study by Medina-Puche et al (2014) showed that F. x ananassa plants subjected to drought stress increased endogenous ABA levels as well as the expression of MYB and anthocyanin accumulation in fruit tissues. Finally, Li et al (2015) showed that silencing the 8 0 -hydroxylase (CYP707A2) gene, which encodes a key enzyme in the oxidative catabolism of ABA, further increased anthocyanin accumulation as well as endogenous ABA levels, and stimulated the expression of the transcription factor MYBA, all compared to the control (without silenced CYP707A2). Consequently, Li et al (2015) suggested that anthocyanin synthesis is tightly regulated by endogenous ABA levels.…”

Section: Induction Mechanism Under Drought Stressmentioning

confidence: 99%

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

González‐Villagra

Kurepin

2017

Planta

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Main conclusion ABA is involved in anthocyanin synthesis through the regulation of microRNA156, augmenting the level of expression of anthocyanin synthesisrelated genes and, therefore, increasing anthocyanin level.Drought stress is the main cause of agricultural crop loss in the world. However, plants have developed mechanisms that allow them to tolerate drought stress conditions. At cellular level, drought stress induces changes in metabolite accumulation, including increases in anthocyanin levels due to upregulation of the anthocyanin biosynthetic pathway. Recent studies suggest that the higher anthocyanin content observed under drought stress conditions could be a consequence of a rise in the abscisic acid (ABA) concentration. This plant hormone crosses the plasma membrane by specific transporters, and it is recognized at the cytosolic level by receptors known as pyrabactin resistance (PYR)/regulatory component of ABA receptors (PYR/RCARs) that regulate downstream components. In this review, we discuss the hypothesis regarding the involvement of ABA in the regulation of microRNA156 (miRNA156), which is upregulated as part of dehydration stress responsiveness in different species. The miRNA156 upregulation produces a greater level of anthocyanin gene expression, forming the multienzyme complex that will synthesize an increased level of anthocyanins at the cytosolic face of the rough endoplasmic reticulum (RER). After synthesis, anthocyanins are transported from the RER to the vacuole by two possible models of transport: (1) membrane vesicle-mediated transport, or (2) membrane transporter-mediated transport. Thus, the aim was to analyze the recent findings on synthesis, transport and the possible mechanism by which ABA could increase anthocyanin synthesis under drought stress conditions potentially throughout microRNA156 (miRNA156).

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PacCYP707A2 negatively regulates cherry fruit ripening while PacCYP707A1 mediates drought tolerance

Abstract: Highlight PacCYP707A2 plays a primary role in regulating ABA levels during the onset of cherry fruit ripening, while PacCYP707A1 regulates the ABA content in response to dehydration.

Cited by 59 publications

References 42 publications

Abscisic acid pathway involved in the regulation of watermelon fruit ripening and quality trait evolution

Abscisic acid pathway involved in the regulation of watermelon fruit ripening and quality trait evolution

Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

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