Transcriptional regulation of hydroxypyruvate reductase gene expression by cytokinin in etiolated pumpkin cotyledons

Andersen, Brian R.; Jin, Ge; Chen, Rui; Ertl, John R.; Chen, Chong-Maw

doi:10.1007/bf00197579

Cited by 23 publications

(8 citation statements)

References 28 publications

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“…There is an increasing body of information on cytokininresponsive genes in higher plants, but genes that are known to be regulated at the transcriptional level are currently limited. These include genes for msr1 (pLS216) (Dominov et al, 1992), hydroxypyruvate reductase (Andersen et al, 1996), chalcone synthase and dihydroflavonol reductase (Deikman and Ulrich, 1995), and C4Ppc1 (Suzuki et al, 1994). Our results clearly demonstrate that ZmCip1 is a cytokinin-inducible gene that is expressed dosedependently at physiological concentrations, requiring no protein synthesis.…”

Section: Discussionmentioning

confidence: 60%

A response‐regulator homologue possibly involved in nitrogen signal transduction mediated by cytokinin in maize

et al. 1998

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SummaryA cDNA clone, pZmCip1, encoding a maize (Zea mays) cytokinin-inducible protein 1 was isolated utilizing the differential display technique, and studied using the expression of ZmCip1 in nitrogen-starved maize plants. The cloned cDNA contained an open reading frame consisting of 157 amino acids with a predicted molecular mass of 16.7 kDa, which possesses similarity with the response-regulators of bacterial two-component signalling systems. In detached leaves, accumulation of ZmCip1 transcript by t-zeatin was dose-dependent in a range of 10 -9 M to 10 -7 M, and occurred within 30 min after treatment. The effect of t-zeatin was replaceable by isopentenyladenosine or isopentenyl-adenosine-5Ј-monophosphate. Pretreatment of detached leaves with cycloheximide did not inhibit the accumulation of the transcript. In whole plants, ZmCip1 transcript was transiently accumulated exclusively in leaves by supply of nitrate or ammonium ions to the roots, whereas the transcript was not accumulated in detached leaves by supply of the nitrogen nutrients. Both the cytokinin-and nitrate-responsive accumulations of ZmCip1 transcript were accompanied by an increase in the immunotitratable protein. Isopentenyladenosine and/or its phosphorylated form(s) accumulated in roots 2 h after supply of nitrate to plants. These results, taken together, suggest that ZmCip1 is a primary response gene to cytokinins, and that it involves, at least in part, the nitrogen-signal transduction mediated by cytokinin in maize.

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Section: Discussionmentioning

confidence: 60%

A response‐regulator homologue possibly involved in nitrogen signal transduction mediated by cytokinin in maize

et al. 1998

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“…Cytokinins also regulate the expression of NADH-HPR gene by acting at the level of transcription and posttranscriptionally. Light is a major factor in the induction of NADH-HPR protein synthesis and the dark causes a strong reduction in its level (Andersen et al 1996). Probably, for this reason the reaction of reduction of hydroxypyruvate to glycerate in dark-grown Chlorella vulgaris is very low.…”

Section: Discussionmentioning

confidence: 98%

Cellular response of light/dark-grown green alga Chlorella vulgaris Beijerinck (Chlorophyceae) to exogenous adenine- and phenylurea-type cytokinins

Piotrowska

Czerpak

2009

Acta Physiol Plant

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Treatment exposed to light Chlorella vulgaris Beijerinck (Chlorophyceae) with adenine-(BA, Kin, Z) and phenylurea-type (DPU) cytokinins effects positively on alga viability by 1.5-to twofold increase in cell number, chlorophylls, carotenoids, monosaccharides and glycolate content as well as NADH-dependent hydroxypyruvate reducing enzyme activity (NADH-HPR) extensively involved in carbon metabolism. Cytokinins enhance nitrogen assimilation by stimulation of NADH-dependent glutamate dehydrogenase (NADH-GDH) aminating activity finally leading to higher protein level and its secretion as well as polypeptide accumulation in the range of molecular weight 12-195 kDa. In the dark, cytokinins mimic the regulatory effect of light upon algal cell division, metabolite content and stimulate carbon recycling for Calvin cycle reactions by enhancing of light-dependent NADH-HPR activity. The delaying of protein degradation and stimulation of their secretion to environment, triggering polypeptide accumulation and twofold higher NADH-GDH activity catalysing amino acids biosynthesis are observed in the dark-grown microalgae in response to cytokinins. Chlorella vulgaris exhibits sensitivity on cytokinins in the following order of their stimulating properties: DPU [ Z [ Kin [ BA in both light and dark conditions. Understanding of cytokinin role in lower plants under different light conditions could be a step toward the elucidation of the evolution of hormone regulation and their action at molecular level.

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“…However, the abundance of the large subunit of Rubisco did not vary appreciably upon cytokinin treatment in our present study, demonstrating once more that transcript amounts do not necessarily reflect protein amounts. Andersen et al (1996) suggested a transcriptional regulation of specific nuclear genes by cytokinin in etiolated, excised Cucurbita cotyledons, whereas Kusnetsov et al (1994) suggested a cytokinin action at the post-transcriptional level in excised Lupinus cotyledons. By analogy to blue-light signal transduction (Reymond et al 1992), this regulation may involve protein phosphorylation, especially as this type of modification is functional in PSII assembly (Allen and Staehelin 1994).…”

Section: Exogenous Cytokinin Cures a Developmental Moss Mutantmentioning

confidence: 96%

Cytokinin affects nuclear- and plastome-encoded energy-converting plastid enzymes

Kasten

Buck

Nuske

et al. 1997

Planta

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Cytokinins induce two specific morphological alterations in mosses: (i) the differentiation of a tip-growing cell into a three-faced apical cell (the so-called bud), and (ii) the division of chloroplasts. In a developmental mutant of the moss Physcomitrella patens (Hedw.) B.S.G. (mutant PC22) impeded in both cellular differentiation (bud production) and chloroplast division, addition of cytokinin (N6-delta 2-isopentenyladenine) led to bud production after 3 d in the wild type and after 7 d in the mutant. Hormone induced a division of the mutant macrochloroplasts starting within 24 h and ongoing for 72 h. During this period the abundances of several plastid proteins changed in both genotypes as judged by two-dimensional-protein gel electrophoresis, silver staining and subsequent quantification with novel computer software. Eight of these polypeptides were isolated independently, subjected to microsequencing and thus identified, resulting in the first protein sequence data from a moss. Three polypeptides (24 kDa, 22 kDa, 20 kDa) were found to be homologous to enhancer protein OEE2 of the oxygen-evolving complex, four to represent isoforms of phosphoglycerate kinase (EC 2.7.2.3), and one was identified as the beta-chain of chloroplast ATPase (EC 3.6.1.34). Possible involvement of these key enzymes of the chloroplast energy-conversion machinery in organelle division and in cellular differentiation is discussed. Further sequence information was obtained from both subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39). Amounts of these polypeptides were not appreciably affected by cytokinin in moss chloroplasts.

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Transcriptional regulation of hydroxypyruvate reductase gene expression by cytokinin in etiolated pumpkin cotyledons

Cited by 23 publications

References 28 publications

A response‐regulator homologue possibly involved in nitrogen signal transduction mediated by cytokinin in maize

A response‐regulator homologue possibly involved in nitrogen signal transduction mediated by cytokinin in maize

Cellular response of light/dark-grown green alga Chlorella vulgaris Beijerinck (Chlorophyceae) to exogenous adenine- and phenylurea-type cytokinins

Cytokinin affects nuclear- and plastome-encoded energy-converting plastid enzymes

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