Transcriptional Regulation of Adipocyte Differentiation: A Central Role for CCAAT/Enhancer-binding Protein (C/EBP) β

Guo, Liang; Li, Xi; Tang, Qi

doi:10.1074/jbc.r114.619957

Cited by 284 publications

(246 citation statements)

References 71 publications

Supporting

Mentioning

230

Contrasting

Unclassified

Order By: Relevance

“…3). C/EBPa plays a very important role during adipocyte terminal differentiation [14]. In the present study, its expression was reduced by WAY-262611 and LiCl (Fig.…”

Section: Wnt/b-catenin Pathway Inhibits Adipogenic Transcription Pattmentioning

confidence: 80%

Curcumin raises lipid content by Wnt pathway in hepatic stellate cell

Zhang

et al. 2016

Journal of Surgical Research

View full text Add to dashboard Cite

“…3). C/EBPa plays a very important role during adipocyte terminal differentiation [14]. In the present study, its expression was reduced by WAY-262611 and LiCl (Fig.…”

Section: Wnt/b-catenin Pathway Inhibits Adipogenic Transcription Pattmentioning

confidence: 80%

Curcumin raises lipid content by Wnt pathway in hepatic stellate cell

Zhang

et al. 2016

Journal of Surgical Research

View full text Add to dashboard Cite

“…After hormonal induction, a rapid and transient increase in transcription and expression of C/EBP␤ and C/EBP␦ is observed in preadipocytes that activates the expression of PPAR␥ and C/EBP␣ (56). The activation of C/EBP␣ is followed by transcriptional activation of many genes encoding proteins involved in creating the adipocyte phenotype (GLUT4, SCD1, LEP, FABP4) (110). C/EBP, a dominant inhibitor of C/EBP␣ and -␤, is induced in late adipocyte differentiation and has been proposed as an inhibitor of adipogenesis.…”

Section: Ontogeny and Function Of White Brown And Beige Adipose Tissuementioning

confidence: 99%

Revisiting the adipocyte: a model for integration of cytokine signaling in the regulation of energy metabolism

Rodrı́guez

Ezquerro

Méndez‐Giménez

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

235

186

View full text Add to dashboard Cite

Adipose tissue constitutes an extremely active endocrine organ with a network of signaling pathways enabling the organism to adapt to a wide range of different metabolic challenges, such as starvation, stress, infection, and short periods of gross energy excess. The functional pleiotropism of adipose tissue relies on its ability to synthesize and release a huge variety of hormones, cytokines, complement and growth factors, extracellular matrix proteins, and vasoactive factors, collectively termed adipokines. Obesity is associated with adipose tissue dysfunction leading to the onset of several pathologies including type 2 diabetes, dyslipidemia, nonalcoholic fatty liver, or hypertension, among others. The mechanisms underlying the development of obesity and its associated comorbidities include the hypertrophy and/or hyperplasia of adipocytes, adipose tissue inflammation, impaired extracellular matrix remodeling, and fibrosis together with an altered secretion of adipokines. Recently, the potential role of brown and beige adipose tissue in the protection against obesity has been also recognized. In contrast to white adipocytes, which store energy in the form of fat, brown and beige fat cells display energy-dissipating capacity through the promotion of triacylglycerol clearance, glucose disposal, and generation of heat for thermogenesis. Identification of the morphological and molecular changes in white, beige, and brown adipose tissue during weight gain is of utmost relevance for the identification of pharmacological targets for the treatment of obesity and its associated metabolic diseases. white and brown adipogenesis; fat browning; adipocyte hypertrophy and hyperplasia; adipose tissue inflammation; extracellular matrix remodeling THE ADIPOSE ORGAN REPRESENTS a special loose connective tissue containing adipocytes and several cell types surrounded by capillary and innervation networks (47, 79, 82). Adipocytes comprise ϳ35-70% of adipose mass, and the other cell types found in the stroma-vascular fraction include preadipocytes, mesenchymal stem cells, macrophages and other immune cells, and endothelial and smooth muscle cells, among others (79). The classical functions of adipose tissue are the storage of energy in the form of triacylglycerols (TG) and as thermal insulator. Adipocytes were formerly classified into two main types: white adipocytes, which store energy in the form of fat, and brown adipocytes, which induce thermogenesis (82). The current classification scheme includes a third category of adipocytes, termed beige or brite (brown-in-white) adipocytes, which can be considered inducible brown-like cells with thermogenic properties (340). Since the identification of leptin in 1994 (359), adipose tissue has emerged as an extremely active endocrine organ that secretes a huge variety of hormones, cytokines, growth factors, and vasoactive factors that are collectively termed adipokines (67, 81,235,262). Over the last three decades, overwhelming evidence has proven that adipokines not only influence adipobiol...

show abstract

“…25) We previously reported that FAD24 acted in concert with HBO1 to promote MCE by controlling DNA replication. 7) In this study, we showed that fad24 is a positive regulator of S phase re-entry of quiescent C2C12 myoblasts.…”

Section: Discussionmentioning

confidence: 99%

Fad24, a Positive Regulator of Adipogenesis, Is Required for S Phase Re-entry of C2C12 Myoblasts Arrested in G0 Phase and Involved in p27<sup>Kip1</sup> Expression at the Protein Level

Ochiai

Nishizuka

Osada

et al. 2016

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Factor for adipocyte differentiation 24 ( fad24) is a positive regulator of adipogenesis. We previously found that human fad24 is abundantly expressed in skeletal muscle. However, the function of fad24 in skeletal muscle remains largely unknown. Because skeletal muscle is a highly regenerative tissue, we focused on the function of fad24 in skeletal muscle regeneration. In this paper, we investigated the role of fad24 in the cell cycle re-entry of quiescent C2C12 myoblasts-mimicked satellite cells. The expression levels of fad24 and histone acetyltransferase binding to ORC1 (hbo1), a FAD24-interacting factor, were elevated at the early phase of the regeneration process in response to cardiotoxin-induced muscle injury. The knockdown of fad24 inhibited the proliferation of quiescent myoblasts, whereas fad24 knockdown did not affect differentiation. S phase entry following serum activation is abrogated by fad24 knockdown in quiescent cells. Furthermore, fad24 knockdown cells show a marked accumulation of p27 Kip1 protein. These results suggest that fad24 may have an important role in the S phase re-entry of quiescent C2C12 cells through the regulation of p27Kip1 at the protein level.Key words muscle regeneration; S phase re-entry; p27 Kip1; satellite cell; C2C12 cell; adipocyte differentiationTo elucidate the molecular mechanism of adipocyte differentiation, we previously isolated 102 genes whose expression was upregulated in the early stage of adipocyte differentiation using the polymerase chain reaction (PCR)-subtraction-cloning method.1,2) Using the rapid amplification of cDNA ends (RACE) and cDNA library screening, we identified five novel genes, factor for adipocyte differentiation ( fad) 24, fad49, fad104, fad123, and fad158. 3-6)The gene fad24 is the mammalian homolog of Noc3, which is involved in DNA replication in yeast. Gain-of-function and loss-of-function experiments have demonstrated that FAD24 promotes adipogenesis by controlling DNA replication. 3,7)Furthermore, to identify the functions of fad24 during adipogenesis in vivo, we generated transgenic (TG) mice.8) The over-expression of fad24 increased the number of smaller adipocytes in white adipose tissue and improved glucose metabolism activity. These results strongly suggest that fad24 plays a crucial role in promoting adipocyte differentiation in vitro and in vivo. Recently, we generated fad24-deficient mice using a gene-targeting strategy and revealed that fad24 is essential for pre-implantation embryos to develop into blastocysts. 9) These results indicate that fad24 is required for not only adipogenesis but also pre-implantation development.We have previously reported that human fad24 is abundantly expressed in the skeletal muscle.3) Furthermore, fad24 promotes the proliferation of C2C12 cells which is a satellitederived myogenic cell line.10) It was reported that a mutation of zebrafish fad24 caused muscle degeneration accompanied by leukocyte infiltration.11) These studies implicated that fad24 plays important roles in skeletal muscles. Howeve...

show abstract

Transcriptional Regulation of Adipocyte Differentiation: A Central Role for CCAAT/Enhancer-binding Protein (C/EBP) β

Cited by 284 publications

References 71 publications

Curcumin raises lipid content by Wnt pathway in hepatic stellate cell

Curcumin raises lipid content by Wnt pathway in hepatic stellate cell

Revisiting the adipocyte: a model for integration of cytokine signaling in the regulation of energy metabolism

Fad24, a Positive Regulator of Adipogenesis, Is Required for S Phase Re-entry of C2C12 Myoblasts Arrested in G0 Phase and Involved in p27<sup>Kip1</sup> Expression at the Protein Level

Contact Info

Product

Resources

About