Transcriptional regulation-mediating ROS homeostasis and physio-biochemical changes in wild tomato (Solanum chilense) and cultivated tomato (Solanum lycopersicum) under high salinity

Kashyap, Sandeep; Kumari, Nishi; Mishra, Pallavi; Moharana, Durga Prasad; Aamir, Mohd; Singh, Bijendra; Prasanna, H. C.

doi:10.1016/j.sjbs.2020.06.032

Cited by 29 publications

(17 citation statements)

References 75 publications

(82 reference statements)

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“…Then, the absorbance of the mixture can be measured at 530 nm by UV–Vis spectrophotometer. The assessment of MDA content was done by following the method described in previous publication [ 41 ]. Briefly, 200 mg fresh samples were homogenized with 5 mL TCA having 0.25% 2-thiobarbituric acid (TBA).…”

Section: Methodsmentioning

confidence: 99%

Cerium oxide nanoparticles improve cotton salt tolerance by enabling better ability to maintain cytosolic K+/Na+ ratio

Liu

Chen

et al. 2021

J Nanobiotechnol

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Background Salinity is a worldwide factor limiting the agricultural production. Cotton is an important cash crop; however, its yield and product quality are negatively affected by soil salinity. Use of nanomaterials such as cerium oxide nanoparticles (nanoceria) to improve plant tolerance to stress conditions, e.g. salinity, is an emerged approach in agricultural production. Nevertheless, to date, our knowledge about the role of nanoceria in cotton salt response and the behind mechanisms is still rare. Results We found that PNC (poly acrylic acid coated nanoceria) helped to improve cotton tolerance to salinity, showing better phenotypic performance, higher chlorophyll content (up to 68% increase) and biomass (up to 38% increase), and better photosynthetic performance such as carbon assimilation rate (up to 144% increase) in PNC treated cotton plants than the NNP (non-nanoparticle control) group. Under salinity stress, in consistent to the results of the enhanced activities of antioxidant enzymes, PNC treated cotton plants showed significant lower MDA (malondialdehyde, up to 44% decrease) content and reactive oxygen species (ROS) level such as hydrogen peroxide (H2O2, up to 79% decrease) than the NNP control group, both in the first and second true leaves. Further experiments showed that under salinity stress, PNC treated cotton plants had significant higher cytosolic K+ (up to 84% increase) and lower cytosolic Na+ (up to 77% decrease) fluorescent intensity in both the first and second true leaves than the NNP control group. This is further confirmed by the leaf ion content analysis, showed that PNC treated cotton plants maintained significant higher leaf K+ (up to 84% increase) and lower leaf Na+ content (up to 63% decrease), and thus the higher K+/Na+ ratio than the NNP control plants under salinity stress. Whereas no significant increase of mesophyll cell vacuolar Na+ intensity was observed in PNC treated plants than the NNP control under salinity stress, suggesting that the enhanced leaf K+ retention and leaf Na+ exclusion, but not leaf vacuolar Na+ sequestration are the main mechanisms behind PNC improved cotton salt tolerance. qPCR results showed that under salinity stress, the modulation of HKT1 but not SOS1 refers more to the PNC improved cotton leaf Na+ exclusion than the NNP control. Conclusions PNC enhanced leaf K+ retention and Na+ exclusion, but not vacuolar Na+ sequestration to enable better maintained cytosolic K+/Na+ homeostasis and thus to improve cotton salt tolerance. Our results add more knowledge for better understanding the complexity of plant-nanoceria interaction in terms of nano-enabled plant stress tolerance. Graphic abstract

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Section: Methodsmentioning

confidence: 99%

Cerium oxide nanoparticles improve cotton salt tolerance by enabling better ability to maintain cytosolic K+/Na+ ratio

Liu

Chen

et al. 2021

J Nanobiotechnol

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“…Hence, it becomes more important to know stress-responsive mechanisms evolved by S. chilense that allows it to survive in life-threatening aridity, high temperature, drought, and salt stress environment in Northern Chile of Atacama Desert 41 . Earlier studies have suggested that S. chilense shows a promising response to drought and salt-tolerance as compared to the cultivated tomato 42 – 44 . Besides these, enhanced salt adaptation and tolerance activities such as mobilization of H 2 O 2 components, higher plant water holding capacity, growth, and increased enzymatic antioxidant capacity are well-documented in S. chilense 42 , 44 – 46 .…”

Section: Introductionmentioning

confidence: 99%

“…Earlier studies have suggested that S. chilense shows a promising response to drought and salt-tolerance as compared to the cultivated tomato 42 – 44 . Besides these, enhanced salt adaptation and tolerance activities such as mobilization of H 2 O 2 components, higher plant water holding capacity, growth, and increased enzymatic antioxidant capacity are well-documented in S. chilense 42 , 44 – 46 . Unfortunately, the information on molecular salt stress mechanisms of this species during salinity is lacking.…”

Section: Introductionmentioning

confidence: 99%

Understanding salt tolerance mechanism using transcriptome profiling and de novo assembly of wild tomato Solanum chilense

Kashyap

Prasanna

Kumari

et al. 2020

Sci Rep

Self Cite

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Soil salinity affects the plant growth and productivity detrimentally, but Solanum chilense, a wild relative of cultivated tomato (Solanum lycopersicum L.), is known to have exceptional salt tolerance. It has precise adaptations against direct exposure to salt stress conditions. Hence, a better understanding of the mechanism to salinity stress tolerance by S. chilense can be accomplished by comprehensive gene expression studies. In this study 1-month-old seedlings of S. chilense and S. lycopersicum were subjected to salinity stress through application of sodium chloride (NaCl) solution. Through RNA-sequencing here we have studied the differences in the gene expression patterns. A total of 386 million clean reads were obtained through RNAseq analysis using the Illumina HiSeq 2000 platform. Clean reads were further assembled de novo into a transcriptome dataset comprising of 514,747 unigenes with N50 length of 578 bp and were further aligned to the public databases. Genebank non-redundant (Nr), Viridiplantae, Gene Ontology (GO), KOG, and KEGG databases classification suggested enrichment of these unigenes in 30 GO categories, 26 KOG, and 127 pathways, respectively. Out of 265,158 genes that were differentially expressed in response to salt treatment, 134,566 and 130,592 genes were significantly up and down-regulated, respectively. Upon placing all the differentially expressed genes (DEG) in known signaling pathways, it was evident that most of the DEGs involved in cytokinin, ethylene, auxin, abscisic acid, gibberellin, and Ca2+ mediated signaling pathways were up-regulated. Furthermore, GO enrichment analysis was performed using REVIGO and up-regulation of multiple genes involved in various biological processes in chilense under salinity were identified. Through pathway analysis of DEGs, “Wnt signaling pathway” was identified as a novel pathway for the response to the salinity stress. Moreover, key genes for salinity tolerance, such as genes encoding proline and arginine metabolism, ROS scavenging system, transporters, osmotic regulation, defense and stress response, homeostasis and transcription factors were not only salt-induced but also showed higher expression in S. chilense as compared to S. lycopersicum. Thus indicating that these genes may have an important role in salinity tolerance in S. chilense. Overall, the results of this study improve our understanding on possible molecular mechanisms underlying salt tolerance in plants in general and tomato in particular.

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“…The positive effects of ET in salt tolerance have been illustrated in A. thaliana (Yang et al ., 2013) and Zea mays (Freitas et al ., 2018). In a study by Kashyap et al ., (2020), S. chilense plants under salt stress coped better than cultivated S. lycopersicum due to a better anti-oxidant system. We also observed high basal levels of the phytohormone ABA in the coastal population LA2932 (Figure S5 and S6).…”

Section: Discussionmentioning

confidence: 99%

Laminarin-triggered defence responses are geographically dependent for natural populations ofSolanum chilense

Kahlon

Förner

Muser

et al. 2021

Preprint

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Natural plant populations are highly polymorphic and often show intraspecific quantitative, variation in resistance properties against pathogens. The activation of the underlying defence responses can depend on the perception of conserved pathogen-associated molecular patterns (PAMPs). To dissect and understand such variation, we evaluated the diversity of responses induced by laminarin (representing a general glucan elicitor of plant immune responses) in the wild tomato species Solanum chilense. We confirm considerable overlap of the plant's global transcriptional responses to laminarin and to the oomycete pathogen Phytophthora infestans. We further measured key components of basal defence responses such as reactive oxygen species (ROS) production and levels of diverse phytohormones and their derivatives upon elicitation with laminarin in 83 plants originating from nine natural populations of S. chilense from distinct geographic origin. We found high diversity in these components at basal and elicitor-induced levels. We generated generalised linear mixed models (GLMMs) with these components to explain the observed resistance phenotype against P. infestans in the plants and found that additive effects of multiple components best explain resistance at the species level. For individual components, we observed the strongest positive correlation between the resistance phenotype and ethylene (ET) production upon laminarin elicitation. The strength of this correlation differed between individual populations. Chemical inhibition of ET production in individuals from a population, in which ET production was associated with resistance, provoked more severe disease symptoms. Our findings reveal high diversity in the strength of induced defence responses within a species and in the basal levels of other stress-related phytohormones. We show the involvement of multiple components with a quantitatively different contribution of individual components to resistance in geographically separated populations of S. chilense against P. infestans.

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Transcriptional regulation-mediating ROS homeostasis and physio-biochemical changes in wild tomato (Solanum chilense) and cultivated tomato (Solanum lycopersicum) under high salinity

Cited by 29 publications

References 75 publications

Cerium oxide nanoparticles improve cotton salt tolerance by enabling better ability to maintain cytosolic K+/Na+ ratio

Cerium oxide nanoparticles improve cotton salt tolerance by enabling better ability to maintain cytosolic K+/Na+ ratio

Understanding salt tolerance mechanism using transcriptome profiling and de novo assembly of wild tomato Solanum chilense

Laminarin-triggered defence responses are geographically dependent for natural populations ofSolanum chilense

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