Transcriptional patterns of<i>Coffea arabica</i>L. nitrate reductase, glutamine and asparagine synthetase genes are modulated under nitrogen suppression and coffee leaf rust

Baba, Viviane Yumi; Braghini, Masako Toma; Santos, Tiago Benedito dos; Carvalho, Kênia Mara Baiocchi de; Soares, João Danillo Moura; Ivamoto, Suzana Tiemi; Maluf, Mirian Perez; Padilha, Lílian; Paccola-Meirelles, Luzia Doretto; Pereira, Luiz Filipe Protásio; Domingues, Douglas Silva

doi:10.7717/peerj.8320

Cited by 9 publications

(6 citation statements)

References 48 publications

(66 reference statements)

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“…Adequate N supply is a key factor for plant resistance to pathogens [ 26 , 27 ]. Excessive N application stimulates plant growth, thus promoting disease occurrence in grain crops and vegetables [ 26 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Glutamine synthetase gene PpGS1.1 negatively regulates the powdery mildew resistance in Kentucky bluegrass

Sun

Xie

Chen

et al. 2022

Horticulture Research

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Excessive nitrogen (N) application may induce powdery mildew (PM) in perennial grasses, but the resistance mechanisms to PM remain unclear. This study evaluated the physiological and molecular mechanisms of PM resistance affected by N supplies in Kentucky bluegrass (Poa pratensis L.). Cultivar ‘Bluemoon’ (N tolerant) and ‘Balin’ (N sensitive) were treated with low N (0.5 mM), normal N (15 mM), and high N (30 mM) for 21 d in a greenhouse. With increasing N levels, the disease growth was more severe in ‘Balin’ than in ‘Bluemoon’. RNA-seq and weighted gene coexpression network analysis revealed that the PpGS1.1 gene encoding glutamine synthetase was a potential hub gene for PM resistance after comparisons across cultivars and N treatments. The N metabolism pathway was connected with the plant-pathogen interaction pathway via PpGS1.1. The expression of PpGS1.1 in rice protoplasts indicated that the protein was located in the nucleus and cytoplasm. Overexpression of PpGS1.1 in wild type Kentucky bluegrass increased carbon and N contents, and the transgenic plants became more susceptible to PM with a lower wax density. The most differentially expressed genes (DEGs) for N metabolism were upregulated and DEGs for fatty acid metabolism pathway were downregulated in the overexpression lines. The results elucidated mechanisms of PM resistance in relation to N metabolism in Kentucky bluegrass.

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Section: Introductionmentioning

confidence: 99%

Glutamine synthetase gene PpGS1.1 negatively regulates the powdery mildew resistance in Kentucky bluegrass

Sun

Xie

Chen

et al. 2022

Horticulture Research

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“…These physical-chemical characteristics are favorable and determinant to ensure yield and low incidence reports of coffee rust and thus maintain important relationships between plants, microorganisms and fungi as reflected in the high content of organic matter ( Baba et al., 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Characterization of Coffea arabica L. parent plants and physicochemical properties of associated soils, Peru

Vigo¹,

Bobadilla²,

Valqui³

et al. 2022

Heliyon

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“…The experiment was conducted in a greenhouse at the Coffee Center ''Alcides Carvalho'', (Instituto Agrono ˆmico de Campinas-IAC, Campinas, Brazil) as described in Baba et al (2020). In this experiment, six months-old plants with uniform leaf size and development were used.…”

Section: Hemileia Vastatrix Infectionmentioning

confidence: 99%

The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants

Santos

Baba

Vieira

et al. 2021

Physiol Mol Biol Plants

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The high costs of N fertilizers in the coffee production emphasizes the need to optimize fertilization practices and improve nitrogen use efficiency. Urea is widespread in nature, characterizing itself as a significant source of nitrogen for the growth and development of several organisms. Thus, the characterization of genes involved in urea transport in coffee plants is an important research topic for the sustainable production of this valuable cash crop. In the current study, we evaluated the expression of the DUR3 gene under abiotic and biotic stresses in coffee plants. Here, we show that the expression of a high-affinity urea transporter gene (CaDUR3) was upregulated by N starvation in leaves and roots of two out of three C. arabica cultivars examined. Moreover, the CaDUR3 gene was differentially expressed in coffee plants under different abiotic and biotic stresses. In plants of cv. IAPAR59, CaDUR3 showed an increased expression in leaves after exposure to water deficit and heat stress, while it was downregulated in plants under salinity. Upon infection with H. vastatrix (coffee rust), the CaDUR3 was markedly up-regulated at the beginning of the infection process in the disease susceptible Catuaı ´Vermelho 99 in comparison with the resistant cultivar. These results indicate that besides urea acquisition and N-remobilization, CaDUR3 gene may be closely involved in the response to various stresses.

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Transcriptional patterns ofCoffea arabicaL. nitrate reductase, glutamine and asparagine synthetase genes are modulated under nitrogen suppression and coffee leaf rust

Cited by 9 publications

References 48 publications

Glutamine synthetase gene PpGS1.1 negatively regulates the powdery mildew resistance in Kentucky bluegrass

Glutamine synthetase gene PpGS1.1 negatively regulates the powdery mildew resistance in Kentucky bluegrass

Characterization of Coffea arabica L. parent plants and physicochemical properties of associated soils, Peru

The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants

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