Transcriptional expression levels and biochemical markers of oxidative stress in the earthworm Eisenia andrei after exposure to 2,4-dichlorophenoxyacetic acid (2,4-D)

Hattab, Sabrine; Boughattas, Iteb; Boussetta, Hamadi; Viarengo, Aldo; Bannı, Mohamed; Sforzini, Susanna

doi:10.1016/j.ecoenv.2015.07.014

Cited by 61 publications

(21 citation statements)

References 41 publications

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“…Peixoto et al, (2006) reported that CAT activity was inhibited and induced when exposed to certain pollutants and concluded that it was not suitable for use as a biomarker in toxicology studies (Jeon et al, 2015). The decrease in the CAT activity in the present study agree with the results obtained by exposing different fish species to different pesticides (Nwani et al, 2010;Hattab et al, 2015). The results indicate that TE lipid peroxidation is accompanied by simultaneous decreases in the activities of antioxidant enzymes such as CAT and SOD.…”

Section: Resultssupporting

confidence: 83%

Temefos’a Maruz Kalan Gökkuşağı Alabalıklarında, Antioksidan Cevaplar

Parlak

Uçar

Alak

et al. 2019

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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In this study, rainbow trout was exposed to temephos (TE) during 96h acute toxicity conditions. The toxic compound was given in doses of 3 mg L-1 and 6 mg L-1. Furthermore, we assessment antioxidant enzymes activity (glucose-6-phosphate dehydrogenase (G6PD), catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD), malondialdehyde (MDA) level in liver and acetylcholinesterase (AChE) in brain. CAT, SOD, GR, G6PD, and MDA in liver were demonstrated significant effects by the treatments (P<0.05). TE exposure resulted in the inhibition of AChE activity, which, compared with the control group, decreased in all doses (P<0.05). The results showed that temephos inhibited liver tissue's enzyme activities and increased MDA levels. In the light of these data, we can say that temephos toxicity should be taken into consideration in aquatic environments.

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Section: Resultssupporting

confidence: 83%

Temefos’a Maruz Kalan Gökkuşağı Alabalıklarında, Antioksidan Cevaplar

Parlak

Uçar

Alak

et al. 2019

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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“…For a correct assessment of soil contamination, the use of bioassays to quickly screen exposure and to show potential effects of contaminants is mandatory. Due to their life-style, earthworms are the first to be exposed to soils contaminants and, therefore, an environmental pollutant occurs earlier and their harmful effects are more evident than in other organisms [6,26,27]. To our knowledge, this is the first study that aims to investigate the effects of aloin exposure on DNA alterations in a species that is relevant to soil ecology.…”

Section: Discussionmentioning

confidence: 99%

“…Our data provided clues about the negative effect of aloin on this biomarker in a dose dependent manner. L/C volume ratio is reported to be a very sensitive cellular marker for evaluating the effects of environmental contaminants on living organisms [6,11]. Stressed cells affected by noxious molecules react by metabolizing cellular macromolecules and organelles.…”

Section: Discussionmentioning

confidence: 99%

“…Earthworms may represent a good allternative for ecotoxicological testing in terrestrial environment, since they are excellent indicators of soil fertility. They are also considered as cosmopolitan and found in many different types of soils and above all, they are vulnerable to soil contaminants [6]. An assortment of data available for comparison executed on Eisenia species, have been demonstrated as substantial toxicity test results [7].…”

Section: Introductionmentioning

confidence: 99%

“…The Micronucleus frequency (MN) has become one of the standard technique for evaluating DNA damage because of its simplicity, sensitivity, speed, and economy [11]. The MN applied to earthworms has been widely used to assess the genotoxicity of heavy metals, pesticides [6], and multi-contaminated field soils [11]. This work aims to evaluate the effect of four sublethal concentrations of aloin crude extracts on growth and DNA damage in the earth worm Eisenia Andrei and to investigate the gene expression regulation of genes involved in DNA repair mechanism; P21 and topoisomerase using quantitative reverse-transcription PCR (qRT-PCR).…”

Section: Introductionmentioning

confidence: 99%

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Evidence of the Genotoxic Effect of Aloin on Earth Worms Eisenia Andrei

Lanouar¹,

Boughattas²,

Mkhinini³

et al. 2017

Foc Sci

Self Cite

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The aim of this work is to investigate the genotoxic effect and related gene expression in the worms Eisenia andrei exposed to four sublethal concentrations of aloin crude extracts (10, 50, 100 and 200 g/kg soils) for 7 and 14 days. Methods: First, worm's growth after the exposure periods was assessed. Micronucleus (MN) frequency in worm's coelomocytes was evaluated for the potential genotoxic effect of aloin. The gene expressions regulations were evaluated with the genes involved in DNA repair: the P21 and topoisomerase, The Lysosomal/cytoplasm ratio was scored as general biomarker of cytotoxicity. Results: Our results demonstrate a significant increase in the MN frequency along the aloin increasing concentrations associated with a significant reduction in worm's growth. Similarly Lysosomal/Cytoplasm ration was highly affected by aloin particularly at higher concentrations and after 14 days of exposure. The gene expression pattern of P21 and Topoisomerase was markedly up-regulated as low concentrations and down-regulated at higher concentrations. Conclusions: Our data should be particularly considered in view of the toxic effect of aloe vera wastes mainly at industrial level.

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Effects of an herbicide on physiology, morphology, and fitness of the dung beetle Euoniticellus intermedius (Coleoptera: Scarabaeidae)

González‐Tokman

Martínez-Morales

Farrera

et al. 2016

Enviro Toxic and Chemistry

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Some agrochemical compounds threaten nontarget organisms and their functions in the ecosystem. The authors experimentally evaluated the effects of one of the most common herbicide mixtures used worldwide, containing 2,4-dichlorophenoxyacetic acid and picloram, on dung beetles, which play fundamental roles in the function of natural and managed ecosystems. The present study employed techniques of physiology and geometric morphometrics, besides including fitness measurements, to assess the effects of the herbicide in the introduced beetle Euoniticellus intermedius. Because herbicide components promote oxidative stress and affect survival in certain insects, the authors predicted negative effects on the beetles. Unexpectedly, no effect of herbicide concentration was found on clutch size, sex ratio, and fluctuating asymmetry, and it even increased physiological condition and body size in exposed beetles. Because the studied species presents 2 male morphs, the authors, for the first time, evaluated the effect of a pollutant on the ratio of these morphs. Contrary to the prediction, the herbicide mixture increased the proportion of major males. Thus, the herbicide does not threaten populations of the studied beetles. The present study discusses how both negative and positive effects of pollutants on wild animals modify natural and sexual selection processes occurring in nature, which ultimately impact population dynamics. The authors recommend the use of physiological and geometric morphometrics techniques to assess the impact of pollutants on nontarget animals. Environ Toxicol Chem 2017;36:96-102. © 2016 SETAC.

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Transcriptional expression levels and biochemical markers of oxidative stress in the earthworm Eisenia andrei after exposure to 2,4-dichlorophenoxyacetic acid (2,4-D)

Cited by 61 publications

References 41 publications

Temefos’a Maruz Kalan Gökkuşağı Alabalıklarında, Antioksidan Cevaplar

Temefos’a Maruz Kalan Gökkuşağı Alabalıklarında, Antioksidan Cevaplar

Evidence of the Genotoxic Effect of Aloin on Earth Worms Eisenia Andrei

Effects of an herbicide on physiology, morphology, and fitness of the dung beetle Euoniticellus intermedius (Coleoptera: Scarabaeidae)

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