Transcriptional control of flavonoid biosynthesis by MYB–bHLH–WDR complexes

Xu, Wenjia; Dubos, Christian; Lepiniec, Loïc

doi:10.1016/j.tplants.2014.12.001

Cited by 1,362 publications

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“…The activity of anthocyanin biosynthesis enzymes is primarily regulated by complexes that consist of R2R3-MYB transcription factors, basic helix-loop-helix (bHLH) transcription factors, and WD40 proteins (Koes et al, 2005;Lai et al, 2013;Xu et al, 2015). In lilies, LhMYB12 (R2R3-MYB) determines tepal-specific anthocyanin biosynthesis together with LhbHLH2 (Nakatsuka et al, 2009) and LhWD40A (Suzuki et al, 2016).…”

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confidence: 99%

LhMYB12, Regulating Tepal Anthocyanin Pigmentation in Asiatic Hybrid Lilies, is Derived from Lilium dauricum and L. bulbiferum

Yamagishi

Nakatsuka

2017

Hortic J

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Genes encoding a MYB12 transcription factor, which regulates anthocyanin biosynthesis, are the key genes causing variations in the anthocyanin colors in lily flowers. However, the origin of the MYB12 in Asiatic hybrid lilies (Lilium spp.) is not completely known. In this study, we analyzed anthocyanin pigments in tepals of L. maculatum, L. lancifolium, L. callosum, L. leichtlinii, L. davidii, L. bulbiferum, and L. dauricum, and clarified that L. dauricum and L. bulbiferum accumulated a single anthocyanin, cyanidin 3-O-rutinoside, in entire tepals, although these wild species had orange-colored tepals. The sequencing of MYB12 genes revealed seven allelic sequences among ten L. dauricum plants and two allelic sequences in one L. bulbiferum plant. These MYB12 sequences were the same as or similar to the sequences isolated from pink Asiatic hybrid lily cultivars, indicating that L. dauricum and L. bulbiferum have contributed to the anthocyanin coloration of these cultivars.

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confidence: 99%

LhMYB12, Regulating Tepal Anthocyanin Pigmentation in Asiatic Hybrid Lilies, is Derived from Lilium dauricum and L. bulbiferum

Yamagishi

Nakatsuka

2017

Hortic J

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“…The main structural genes of anthocyanin biosynthesis include phenylalanine ammonia lyase, chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase, flavonoid 3-hydroxylase, dihydroflavonol 4-reductase, leucoanthocyanidin dioxygenase, and flavonoid 3-O-glucosyltransferase (Dunlop and Curtis 1991). The metabolism and regulation of anthocyanin biosynthesis are mainly achieved through the regulated expression of structural genes (Gion et al 2012;Xu et al 2015), and three types of transcription factors, MYB, bHLH, and WD40, regulate the expression of these structural genes (Broun 2005;Koes et al 2005). The allelic variation in the MYB genes plays a key role in the color differentiation of natural plant populations.…”

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confidence: 99%

TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

Zong

Liu

et al. 2017

Cereal Research Communications

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Purple pericarp is an interesting and useful trait in Triticum aestivum, but the molecular mechanism behind this phenotype remains unclear. The allelic variation in the MYB transcriptors is associated with the phenotype of pigmented organs in many plants. In this study, a MYB transcription factor gene, TaMYB3, was isolated using homology-based cloning and a differentially expressed gene mining approach, to verify the function of the MYB transcriptor in the purple pericarp. The coding sequence of TaMYB3 in cultivar Gy115 was the same as that in cultivar Opata. TaMYB3 was localized to FL0.62-0.95 on chromosome 4BL. The TaMYB3 protein contains DNA-binding and transcription-activation domains, and clustered on a phylogenetic tree with the MYB proteins that regulates anthocyanin and proanthocyanin biosynthesis. TaMYB3 localized in the nuclei of Arabidopsis thaliana and wheat protoplasts after it was transiently expressed with PEG transformation. TaMYB3 induced anthocyanin synthesis in the pericarp cells of Opata in the dark in collaboration with the basic helix-loop-helix protein ZmR, which is also the function of ZmC1. However, TaMYB3 alone did not induce anthocyanin biosynthesis in the pericarp cells of the white grain wheat cultivar Opata in the light after bombardment, whereas the single protein ZmR did. Light increased the expression of TaMYB3 in the pericarp of Gy115 and Opata, but only induced anthocyanin biosynthesis in the grains of Gy115. Our results extend our understanding of the molecular mechanism of the purple pericarp trait in T. aestivum.

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“…PAs are potentially attractive compounds in terms of human health because of their scavenging capacity for free radicals; PAs are also known to confer astringency or bitterness to fruit and vegetables (Aron and Kennedy, 2008;Bagchi et al, 2014;Dixon et al, 2005). The commercial and biological significance of PA biosynthesis has resulted in extensive studies being conducted on the PA biosynthetic pathway and its transcriptional regulators, especially in the model plant Arabidopsis thaliana (Lepiniec et al, 2006;Saito et al, 2013;Xu et al, 2015). Persimmon (Diospyros kaki Thunb.…”

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Comparative Mapping of the ASTRINGENCY Locus Controlling Fruit Astringency in Hexaploid Persimmon (Diospyros kaki Thunb.) with the Diploid D. lotus Reference Genome

et al. 2018

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Persimmon (Diospyros kaki) is a tree crop species that originated in East Asia, consists mainly of hexaploid individuals (2n = 6x = 90) with some nonaploid individuals. One of the unique characteristics of persimmon is the continuous accumulation of proanthocyanidins (PAs) in its fruit until the middle of fruit development, resulting in a strong astringent taste even at commercial fruit maturity. Among persimmon cultivars, pollination-constant and non-astringent (PCNA) types cease PA accumulation in early fruit development and become non-astringent at commercial maturity. PCNA is an allelic trait to non-PCNA and is controlled by a single locus called the ASTRINGENCY (AST) locus. Previous segregation analyses indicated that the AST locus shows hexasomic inheritance; a recessive allele, ast, at this locus confers PCNA. Here, we report a shuttle mapping approach to delimit the AST locus region in the hexaploid persimmon genome by using D. lotus, a diploid relative of D. kaki, as a reference. A D. lotus F 1 population of 333 individuals and 296 D. kaki siblings segregating for the PCNA trait were used to map the AST region using haplotype-specific markers covering the AST region. This indicated that the AST locus is syntenic to an approximately 915-kb region of the D. lotus genome. In this 915-kb region, we found several candidates for AST that were revealed from the fruit transcriptome of a population segregating for the PCNA trait. These results could provide important clues for the isolation of AST in hexaploid persimmon.Key Words: candidate gene, chromosome walking, polyploidy, proanthocyanidins. IntroductionProanthocyanidins (PAs) are a class of final products of the flavonoid biosynthetic pathway that generally have protective roles against biotic and abiotic stresses. PAs are potentially attractive compounds in terms of human health because of their scavenging capacity for free radicals; PAs are also known to confer astringency or bitterness to fruit and vegetables (Aron and Kennedy, 2008;Bagchi et al., 2014;Dixon et al., 2005). The commercial and biological significance of PA biosynthesis has resulted in extensive studies being conducted on the PA biosynthetic pathway and its transcriptional regulators, especially in the model plant Arabidopsis thaliana (Lepiniec et al., 2006;Saito et al., 2013;Xu et al., 2015). Persimmon (Diospyros kaki Thunb.), an economically important fruit crop cultivated in temperate and Mediterranean climates, shows a distinctive characteristic of PA accumulation in its fruit. In persimmon fruit, the mean degree of polymerization of PA is high (approximately 10-40), and unlike other important fruit crops, persimmon fruit accumulates a vast amount of PA until the middle of fruit development, resulting in a This article is an Advance Online Publication of the authors' corrected proof. Note that minor changes may be made before final version publication. strong astringent taste even at commercial maturity (Akagi et al., 2009a(Akagi et al., , 2011. Depending on the genotype, persimmon fru...

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Transcriptional control of flavonoid biosynthesis by MYB–bHLH–WDR complexes

Cited by 1,362 publications

References 135 publications

<i>LhMYB12</i>, Regulating Tepal Anthocyanin Pigmentation in Asiatic Hybrid Lilies, is Derived from <i>Lilium dauricum</i> and <i>L. bulbiferum</i>

<i>LhMYB12</i>, Regulating Tepal Anthocyanin Pigmentation in Asiatic Hybrid Lilies, is Derived from <i>Lilium dauricum</i> and <i>L. bulbiferum</i>

TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

Comparative Mapping of the <i>ASTRINGENCY</i> Locus Controlling Fruit Astringency in Hexaploid Persimmon (<i>Diospyros kaki</i> Thunb.) with the Diploid <i>D. lotus</i> Reference Genome

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