Transcriptional Changes Caused by Bisphenol A in  Oryzias javanicus, a Fish Species Highly Adaptable to Environmental Salinity

Woo, Seonock; Denis, Vianney; Yum, Seungshic

doi:10.3390/md12020983

Cited by 13 publications

(7 citation statements)

References 39 publications

(39 reference statements)

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“…Glutathione-S-transferase is a phase II metabolic detoxification enzyme. Woo et al (2014) reported increase in GST activity in Oryzias javanicus after exposure to BPA in sea water. Activity of GST also increased in kidney and liver of grass carp after exposure to 3 mg/l of BPA (Faheem and Lone 2017).…”

Section: Discussionmentioning

confidence: 96%

Bisphenol-A induced antioxidants imbalance and cytokines alteration leading to immune suppression during larval development of Labeo rohita

Faheem

Adeel

Khaliq

et al. 2020

Environ Sci Pollut Res

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Recently, the oxidative stress and immunotoxicity biomarkers have been extensively used in embryotoxicity using fish embryos as promising models especially after exposure to chemical-like environmental estrogens. Bisphenol-A (BPA) is an estrogenic endocrine disruptor and is ubiquitous in the aquatic environment. Larvae of Labeo rohita were exposed to low concentrations of BPA (10, 100, 1000 μg/l) for 21 days. Innate immune system, antioxidants parameters, and developmental alterations were used as biomarkers. Exposure to BPA caused developmental abnormalities including un-inflated swim bladder, delayed yolk sac absorption, spinal curvature, and edema of pericardium. Lipid peroxidation increased and activity of catalase (p < 0.05), superoxide dismutase (p < 0.05), and glutathione peroxidase (p < 0.01) decreased after exposure to BPA. Level of reduced glutathione also decreased (p < 0.05) in BPA-exposed group. Lower expression of tumor necrosis factor-α (p < 0.05) and interferon-γ (p < 0.001) was observed in BPA-exposed groups while expression of interleukin-10 increased (p < 0.05) in larvae exposed to 10 μg/l BPA. Moreover, exposure of BPA caused a concentration-dependent increase in expression of heat shock protein 70 (p < 0.05). The present study showed that the exposure to BPA in early life stages of Labeo rohita caused oxidative stress and suppress NF-κB signaling pathway leading to immunosuppression. The results presented here demonstrate the cross talk between heat shock protein 70 and cytokines expression.

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Section: Discussionmentioning

confidence: 96%

Bisphenol-A induced antioxidants imbalance and cytokines alteration leading to immune suppression during larval development of Labeo rohita

Faheem

Adeel

Khaliq

et al. 2020

Environ Sci Pollut Res

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“…This may be explained by the greater hardness and thickness of the egg shell of marine medaka embryos than zebrafish embryos and the longer incubation period (11 days compared with 48 h), resulted in greater protection of the embryo by the egg shell during medaka development 30,31 . In addition, salinity influences the toxic effect of pollutants 20,32 . In this study, exposure to 1 mg/L and 5 mg/L BPAF caused a hatching delay in marine medaka embryos and reduced the hatching rate.…”

Section: Discussionmentioning

confidence: 62%

“…These residual BPs threaten the health of local aquatic species and humans 17 . Furthermore, the salinity of the water in the estuary area may affect the toxic effects of BPs, including BPAF, on aquatic organisms 20 …”

Section: Introductionmentioning

confidence: 99%

Toxic effects of bisphenol AF on the embryonic development of marine medaka (Oryzias melastigma)

Huang

Gao

Chen

et al. 2023

Environmental Toxicology

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Bisphenol AF (BPAF), an emerging environmental endocrine disruptor, has been detected in surface waters worldwide and has adverse effects on aquatic organisms. The accumulation of BPAF in oceans and its potential toxic effect on marine organisms are important concerns. In this study, the effects of BPAF (10, 100, 1, and 5 mg/L) on marine medaka (Oryzias melastigma) were evaluated, including effects on the survival rate, heart rate, hatchability, morphology, and gene expression in embryos. The survival rate of marine medaka embryos was significantly lower after treatment with 5 mg/L BPAF than in the solvent control group. Exposure to 1 mg/L and 5 mg/L BPAF significantly reduced hatchability. Low‐dose BPAF (10 μg/L) significantly accelerated the heart rate of embryos, while high‐dose BPAF (5 mg/L) significantly decreased the heart rate. BPAF exposure also resulted in notochord curvature, pericardial edema, yolk sac cysts, cardiovascular bleeding, and caudal curvature in marine medaka. At the molecular level, BPAF exposure affected the transcript levels of genes involved in the thyroid system (dio1, dio3a, trhr2, tg, and thra), cardiovascular system (gata4, atp2a1, and cacna1da), nervous system (elavl3 and gap43), and antioxidant and inflammatory systems (sod, pparβ, and il‐8) in embryos. These results indicate that BPAF exposure can alter the expression of functional genes, induce abnormal development, and reduce the hatching and survival rates in marine medaka embryos. Overall, BPAF can adversely affect the survival and development of marine medaka embryos, and BPAF may not be an ideal substitute for BPA.

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“…The nonspecific nature of such effects of environmental stress on macromolecular damage and the induction of upp1 during acute heat stress in black rockfish ( Sebastes schlegelii ) supports its role for replenishing building blocks for RNA pools during stress [ 86 ]. Moreover, the upp2 gene of Javanese medaka ( Oryzias javanicus ), was shown to be induced by yet another type of stress, bisphenol A (BPA), which is a potent environmental toxicant, implicating upp2 in the compensation of BPA chemical toxicant stress [ 87 ].…”

Section: Discussionmentioning

confidence: 99%

Prediction and Experimental Validation of a New Salinity-Responsive Cis-Regulatory Element (CRE) in a Tilapia Cell Line

Kim

Wang

Kültz

2022

Life

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Transcriptional regulation is a major mechanism by which organisms integrate gene x environment interactions. It can be achieved by coordinated interplay between cis-regulatory elements (CREs) and transcription factors (TFs). Euryhaline tilapia (Oreochromis mossambicus) tolerate a wide range of salinity and thus are an appropriate model to examine transcriptional regulatory mechanisms during salinity stress in fish. Quantitative proteomics in combination with the transcription inhibitor actinomycin D revealed 19 proteins that are transcriptionally upregulated by hyperosmolality in tilapia brain (OmB) cells. We searched the extended proximal promoter up to intron1 of each corresponding gene for common motifs using motif discovery tools. The top-ranked motif identified (STREME1) represents a binding site for the Forkhead box TF L1 (FoxL1). STREME1 function during hyperosmolality was experimentally validated by choosing two of the 19 genes, chloride intracellular channel 2 (clic2) and uridine phosphorylase 1 (upp1), that are enriched in STREME1 in their extended promoters. Transcriptional induction of these genes during hyperosmolality requires STREME1, as evidenced by motif mutagenesis. We conclude that STREME1 represents a new functional CRE that contributes to gene x environment interactions during salinity stress in tilapia. Moreover, our results indicate that FoxL1 family TFs are contribute to hyperosmotic induction of genes in euryhaline fish.

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Transcriptional Changes Caused by Bisphenol A in Oryzias javanicus, a Fish Species Highly Adaptable to Environmental Salinity

Cited by 13 publications

References 39 publications

Bisphenol-A induced antioxidants imbalance and cytokines alteration leading to immune suppression during larval development of Labeo rohita

Bisphenol-A induced antioxidants imbalance and cytokines alteration leading to immune suppression during larval development of Labeo rohita

Toxic effects of bisphenol AF on the embryonic development of marine medaka (Oryzias melastigma)

Prediction and Experimental Validation of a New Salinity-Responsive Cis-Regulatory Element (CRE) in a Tilapia Cell Line

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