Transcriptional analysis of scar-free wound healing during early stages of tail regeneration in the green anole lizard, Anolis carolinensis

Xu, Cindy; Hutchins, Elizabeth; Tokuyama, Minami; Wilson‐Rawls, Jeanne; Kusumi, Kenro

doi:10.1016/j.regen.2019.100025

Cited by 15 publications

(30 citation statements)

References 287 publications

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“…Table 1) also points out several major signaling pathway components, in addition to the Wnt--catenin pathway already mentioned, notably Jak/STAT [113,141,142], Notch [126,[143][144][145], and MAPK [108,142,143,146], in particular downstream of FGF/FGFR signaling [120,123,147,148], which are dynamically expressed in various regeneration contexts and steps (Figure 4). This analysis also unveils the importance of less-studied regulators, such as those linked to epigenetic modifications [112,143,149], non-coding RNAs [146,147], and importantly many unknown novel or species-specific regeneration genes [107,113,122,141,147,150].…”

Section:  Evolutionary History Of Regenerationmentioning

confidence: 81%

Animal regeneration in the era of transcriptomics

Bideau

Kerner

Hui

et al. 2021

Cell. Mol. Life Sci.

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Animal regeneration, the ability to restore a lost body part, is a process that has fascinated scientists for centuries. In this review, we first present what regeneration is and how it relates to development, as well as the widespread and diverse nature of regeneration in animals.Despite this diversity, animal regeneration includes three common mechanistic steps: initiation, induction and activation of progenitors, and morphogenesis. In this review article, we summarize and discuss from an evolutionary perspective the recent data obtained for a

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Section:  Evolutionary History Of Regenerationmentioning

confidence: 81%

Animal regeneration in the era of transcriptomics

Bideau

Kerner

Hui

et al. 2021

Cell. Mol. Life Sci.

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“…Furthermore, the initial injury and immunological response is critical in non-avian reptile regeneration 132,133 . Previous transcriptomic analyses in the green anole lizard revealed many genes involved in the innate and adaptive immune response as well as genes enriched for extracellular matrix remodeling, wound epidermis formation, and re-innervation, which together inhibit fibrosis and initiate the regenerative program 134 . However, in our study, all individuals analyzed in this study were either juveniles or sub-adults, raising the question of whether adult alligators exhibit the same repair with regrowth capacity after tail amputation.…”

Section: Discussionmentioning

confidence: 99%

Anatomical and histological analyses reveal that tail repair is coupled with regrowth in wild-caught, juvenile American alligators (Alligator mississippiensis)

Palade

Fisher

et al. 2020

Sci Rep

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Reptiles are the only amniotes that maintain the capacity to regenerate appendages. This study presents the first anatomical and histological evidence of tail repair with regrowth in an archosaur, the American alligator. The regrown alligator tails constituted approximately 6–18% of the total body length and were morphologically distinct from original tail segments. Gross dissection, radiographs, and magnetic resonance imaging revealed that caudal vertebrae were replaced by a ventrally-positioned, unsegmented endoskeleton. This contrasts with lepidosaurs, where the regenerated tail is radially organized around a central endoskeleton. Furthermore, the regrown alligator tail lacked skeletal muscle and instead consisted of fibrous connective tissue composed of type I and type III collagen fibers. The overproduction of connective tissue shares features with mammalian wound healing or fibrosis. The lack of skeletal muscle contrasts with lizards, but shares similarities with regenerated tails in the tuatara and regenerated limbs in Xenopus adult frogs, which have a cartilaginous endoskeleton surrounded by connective tissue, but lack skeletal muscle. Overall, this study of wild-caught, juvenile American alligator tails identifies a distinct pattern of wound repair in mammals while exhibiting features in common with regeneration in lepidosaurs and amphibia.

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“…In recent years, several lizard genomes have been characterized, including that of the green lizard Anolis carolinensis , the primary candidate for regeneration studies [ 19 ]. This allowed to carry out genomic and transcriptomic analysis of genes involved in wound healing, cell proliferation and inflammatory-immune response to injury [ 20 , 21 , 22 , 23 , 24 ]. Nonetheless, transgenic or knockout/knockdown lizard models are still difficult to generate due to the complexity of accessing zygotes for genetic manipulation.…”

Section: Experimental Approachesmentioning

confidence: 99%

Appendage Regeneration in Vertebrates: What Makes This Possible?

Daponte

Tylżanowski

Forlino

2021

Cells

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The ability to regenerate amputated or injured tissues and organs is a fascinating property shared by several invertebrates and, interestingly, some vertebrates. The mechanism of evolutionary loss of regeneration in mammals is not understood, yet from the biomedical and clinical point of view, it would be very beneficial to be able, at least partially, to restore that capability. The current availability of new experimental tools, facilitating the comparative study of models with high regenerative ability, provides a powerful instrument to unveil what is needed for a successful regeneration. The present review provides an updated overview of multiple aspects of appendage regeneration in three vertebrates: lizard, salamander, and zebrafish. The deep investigation of this process points to common mechanisms, including the relevance of Wnt/β-catenin and FGF signaling for the restoration of a functional appendage. We discuss the formation and cellular origin of the blastema and the identification of epigenetic and cellular changes and molecular pathways shared by vertebrates capable of regeneration. Understanding the similarities, being aware of the differences of the processes, during lizard, salamander, and zebrafish regeneration can provide a useful guide for supporting effective regenerative strategies in mammals.

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Transcriptional analysis of scar-free wound healing during early stages of tail regeneration in the green anole lizard, Anolis carolinensis

Cited by 15 publications

References 287 publications

Animal regeneration in the era of transcriptomics

Animal regeneration in the era of transcriptomics

Anatomical and histological analyses reveal that tail repair is coupled with regrowth in wild-caught, juvenile American alligators (Alligator mississippiensis)

Appendage Regeneration in Vertebrates: What Makes This Possible?

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