Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance

Balbontín, Cristián; Ayala, H. G.; Rubilar, Joselyn; Cote, Jessica L.; Figueroa, Carlos R.

doi:10.4067/s0718-58392014000200006

Cited by 61 publications

(49 citation statements)

References 36 publications

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“…The accumulation of galacturonate in skin tissues could be associated with the activation of βgalactosidase and the solubilization of pectin [34]. This observation is consistent with results previously obtained [35], who found that the up-regulation of various cell-wall genes, such as βgalactosidase in sweet cherry fruit contribute to a greater flexibility and elasticity of the skin, which in turn is reflected by a lower percent of cracking. In this regard, cracking-susceptible sweet cherry cultivars release higher level of soluble pectin fractions during hydrocooling conditions [25].…”

Section: Discussionsupporting

confidence: 91%

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Michailidis

Karagiannis

Tanou³

et al. 2020

Preprint

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Background: Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivocracking assay, the 'Christensen method' (cracking evaluation following fruit immersion in water) is commonly used; however, this test does not adequately simulate environmental conditions.Herein, we have designed and evaluated a cracking protocol, named 'Waterfall method', in which fruits are continuously wetted under controlled conditions. Results: The application of this method alone, or in combination with 'Christensen method, was shown to be a reliable approach to characterize sweet cherry cracking behavior. Seventeen cherry cultivars were tested for their cracking behavior using both protocols, and primary as well as secondary metabolites identification was performed in skin tissue using a combined GC-MS and UPLC-MS/MS platform. Significant variations of some of the detected metabolites were discovered and important cracking index-metabolite correlations were identified. Conclusions:We have established an alternative/complementary method of cherry cracking characterization alongside to Christiansen assay.Additional file 1: Supplementary Table S1. Physiological traits of seventeen cherry cultivars at harvest and MANOVA output. Supplementary Table S2. Texture properties, main cracking of cultivars at harvest and MANOVA output. Additional file 2:Additional file 3: Supplementary Table S3. Quantitative results of skin primary metabolite analysis and MANOVA output. Supplementary Table S4. Quantitative results of skin secondary metabolite analysis and MANOVA output. Additional file 4:

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Section: Discussionsupporting

confidence: 91%

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Michailidis

Karagiannis

Tanou³

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…The accumulation of galacturonate in skin tissues could be associated with the activation of βgalactosidase and the solubilization of pectin [31]. This observation is consistent with results previously obtained [32], who found that the up-regulation of various cell-wall genes, such as βgalactosidase in sweet cherry fruit contribute to a greater flexibility and elasticity of the skin, which in turn is reflected by a lower percent of cracking. In this regard, cracking-susceptible sweet cherry cultivars release higher level of soluble pectin fractions during hydrocooling conditions [22].…”

Section: Physiological Traits and Skin Metabolites In Relations To Crsupporting

confidence: 91%

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Michailidis

Karagiannis

Tanou³

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivo cracking assay, the ‘Christensen method’ (cracking evaluation following fruit immersion in water) is commonly used; however, this test has been questioned. Herein, we have designed and evaluated a cracking protocol, named ‘Waterfall method’, in which fruits are continuously wetted under controlled conditions. Results: The application of this method alone, or in combination with ‘Christensen method, was shown to be a reliable approach to characterize sweet cherry cracking behavior. Seventeen cherry cultivars were tested for their cracking behavior using both protocols, and primary as well as secondary metabolites identification was performed in skin tissue using a combined GC-MS and UPLC-MS/MS platform. Significant variations of some of the detected metabolites were discovered and important cracking index–metabolite correlations were identified. Conclusions: We have established an efficient cracking protocolwhich may facilitate breeding for new sweet cherry cultivars with high resistance tocracking.

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“…To evaluate cracking tolerance, fruit of similar size from different treatments were sorted visually to remove any damaged fruit. A set of 25 stem-attached fruits from each biological sample (tree) was placed in distilled water at 20 °C for 6 h. Cracking index (CI) was calculated by the method used by Balbontín et al (2014), based in the formula reported by Christensen (1972). CI = ((6a + 5b + 4c + 3d + 2e+1f) (MPV)-1) 100 where a, b, c, d, e and f represent the number of cracked fruit at 1, 2, 3, 4, 5 and 6 h respectively, MPV is maximum possible value (25 fruits × 6 h).…”

Section: Cracking Tolerance and Fruit Quality Assessmentsmentioning

confidence: 99%

Effect of abscisic acid and methyl jasmonate preharvest applications on fruit quality and cracking tolerance of sweet cherry

Balbontín

Gutiérrez²,

Wolff

et al. 2018

Chil. j. agric. res.

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Rain-induced cherry fruit cracking is one of the most important problems in the cherry industry, and its occurrence causes significant economic losses. Sweet cherry (Prunus avium [L.] L.) is a non-climacteric fruit affected by both abscisic acid (ABA) and methyl jasmonate (MeJA) during development. The objective of this study was to evaluate the effect of these phytohormones on cracking susceptibility and quality parameters of sweet cherry fruit ('Bing'), located in the central region of Chile. During two seasons, independent pre-harvest applications of ABA (0.1 mM) and MeJA (0.4 mM) or both combined, at fruit developmental stages of fruit set or fruit color change, significantly reduced the number of mature cracked fruit after 6 h of immersion in water (p < 0.05). In both seasons the combinations of ABA and MeJA applied at fruit set reducing cracking index in an 87% compared to the control without compromising the weight or the diameter of the fruits. Moreover, in the second season ABA and MeJA applications at fruit set increased fruit firmness (11% and 6% respectively) and fruit color parameters regardless of the fruit stage at application, although slight decreases in soluble solids content were observed in most of the treatments.

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Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance

Cited by 61 publications

References 36 publications

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Effect of abscisic acid and methyl jasmonate preharvest applications on fruit quality and cracking tolerance of sweet cherry

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