Transcriptional activation of the parsley chalcone synthase promoter in heterologous pea and yeast systems

Kalbin, Georgi; Strid, Åke; Frohnmeyer, Hanns

doi:10.1016/s0981-9428(99)00116-3

Cited by 2 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The quantity of plasmid needed for each transformation experiment (10–75 μg depending on the system used; Dangl et al. , 1987; Kalbin et al. 1999; Kircher et al.…”

Section: Resultsmentioning

confidence: 99%

“…The quantity of plasmid needed for each transformation experiment (10-75 lg depending on the system used; Dangl et al, 1987;Kalbin et al 1999;Kircher et al 1999) is the principal drawback of the use of protoplasts, especially when employing low-copy binary vectors. Thus, to overcome this problem, we designed two small pBluescript-based vectors that are highly expressed in Escherichia coli, allowing between 30 and 60 transformations when extracted from a 100-ml bacterial culture (up to 300 lg of plasmid).…”

Section: Physcomitrella Patens Protoplast Production Transformation mentioning

confidence: 99%

See 1 more Smart Citation

A new system for fast and quantitative analysis of heterologous gene expression in plants

Thévenin

Dubos

et al. 2011

New Phytologist

View full text Add to dashboard Cite

Summary• Large-scale analysis of transcription factor-cis-acting element interactions in plants, or the dissection of complex transcriptional regulatory mechanisms, requires rapid, robust and reliable systems for the quantification of gene expression.• Here, we describe a new system for transient expression analysis of transcription factors, which takes advantage of the fast and easy production and transfection of Physcomitrella patens protoplasts, coupled to flow cytometry quantification of a fluorescent protein (green fluorescent protein). Two small-sized and high-copy GatewayÒ vectors were specifically designed, although standard binary vectors can also be employed.• As a proof of concept, the regulation of BANYULS (BAN), a key structural gene involved in proanthocyanidin biosynthesis in Arabidopsis thaliana seeds, was used. In P. patens, BAN expression is activated by a complex composed of three proteins (TT2 ⁄ AtMYB123, TT8 ⁄ bHLH042 and TTG1), and is inhibited by MYBL2, a transcriptional repressor, as in Arabidopsis. Using this approach, two new regulatory sequences that are necessary and sufficient for specific BAN expression in proanthocyanidin-accumulating cells were identified.• This one hybrid-like plant system was successfully employed to quantitatively assess the transcriptional activity of four regulatory proteins, and to identify their target recognition sites on the BAN promoter.

show abstract

“…The quantity of plasmid needed for each transformation experiment (10–75 μg depending on the system used; Dangl et al. , 1987; Kalbin et al. 1999; Kircher et al.…”

Section: Resultsmentioning

confidence: 99%

Section: Physcomitrella Patens Protoplast Production Transformation mentioning

confidence: 99%

A new system for fast and quantitative analysis of heterologous gene expression in plants

Thévenin

Dubos

et al. 2011

New Phytologist

View full text Add to dashboard Cite

show abstract

Protoplasts of Grain and Forage Legumes: Their Exploitation in Genetic Manipulation, Physiological Investigations and Plant-Pathogen Interactions

Davey

Marchant

Power

2003

Focus on Biotechnology

View full text Add to dashboard Cite

Transcriptional activation of the parsley chalcone synthase promoter in heterologous pea and yeast systems

Cited by 2 publications

References 29 publications

A new system for fast and quantitative analysis of heterologous gene expression in plants

A new system for fast and quantitative analysis of heterologous gene expression in plants

Protoplasts of Grain and Forage Legumes: Their Exploitation in Genetic Manipulation, Physiological Investigations and Plant-Pathogen Interactions

Contact Info

Product

Resources

About