Protoplasts of Grain and Forage Legumes: Their Exploitation in Genetic Manipulation, Physiological Investigations and Plant-Pathogen Interactions

Davey, M. R.; Marchant, R.; Power, J. B.

doi:10.1007/978-94-017-0109-9_5

Cited by 3 publications

(1 citation statement)

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“…During the last decades, the isolation and culture of protoplasts have been described for grain legumes of the genera Cajanus, Lupinus, Pisum and Vigna (Davey et al 2003). A major bottleneck in using protoplast fusion, though, is lack of efficient in vitro regeneration protocols.…”

Section: Introductionmentioning

confidence: 99%

Protoplast isolation and culture for somatic hybridization of Lupinus angustifolius and L. subcarnosus

Sonntag

Ruge-Wehling

Wehling

2008

Plant Cell Tiss Organ Cult

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A method for isolation and shoot regeneration from electrofused protoplasts of L. angustifolius and L. subcarnosus was developed. Viable protoplasts were isolated from leaves of in-vitro grown seedlings at an average yield of 6 9 10 5 protoplasts g -1 fresh weight. Liquid and agarose solidified B5 media were used for protoplast culture. In the liquid-culture system, all tested media, VKM, P1 and KM8p, were applicable for inducing cell division (84% of all tested petri dishes at four weeks) and colony formation. Media containing additional carbohydrates were suitable to produce compact calli with green and brown pigmentations in different combinations. Analysis of callus with molecular markers allowed to identify six somatic hybrids. However, none of the parental-protoplast derived cell colonies could develop shoots. This is the first report on protoplast fusion of L. angustifolius and L. subcarnosus with subsequent shoot regeneration.

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Section: Introductionmentioning

confidence: 99%