Transcription Factors PvERF15 and PvMTF-1 Form a Cadmium Stress Transcriptional Pathway

Lin, Tao; Yang, Wanning; Wen, Lijun; Wāng, Ying; Qi, Xiaoting

doi:10.1104/pp.16.01729

Cited by 65 publications

(46 citation statements)

References 24 publications

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“…A number of studies reveal that the ERF subfamily members in various plant species, acting as either transcriptional activators or repressors, play a pivotal role in regulation of a range of physiological and biological processes, including internode elongation (Zhou et al ., ), root growth (Jung et al ., ), trichome formation (Sun et al ., ), fruit ripening (Yin et al ., ), hormone signal transduction (Rashotte et al ., ), secondary metabolism and biotic stress response (Zeng et al ., ; Zhu et al ., ). Accumulating evidence demonstrate that ERFs are also implicated in response to abiotic stresses, including submergence or hypoxia (Xu et al ., ), heavy metal (Lin et al ., ), drought (Jung et al ., ) and high salinity (Yao et al ., ). ERFs have been also demonstrated to be responsive to cold stress.…”

Section: Introductionmentioning

confidence: 99%

ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process

Wang

Dai

et al. 2019

Plant Biotechnology Journal

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Summary Ethylene‐responsive factors ( ERF s) have been revealed to play essential roles in a variety of physiological and biological processes in higher plants. However, functions and regulatory pathways of most ERF s in cold stress remain largely unclear. Here, we identified Ptr ERF 109 of trifoliate orange ( Poncirus trifoliata (L.) Raf.) and deciphered its role in cold tolerance. Ptr ERF 109 was drastically up‐regulated by cold, ethylene and dehydration, but repressed by salt. Ptr ERF 109 was localized in the nucleus and displayed transcriptional activity, and the C terminus is required for the activation. Overexpression of Ptr ERF 109 conferred enhanced cold tolerance in transgenic tobacco and lemon plants, whereas VIGS (virus‐induced gene silencing)‐mediated suppression of Ptr ERF 109 in trifoliate orange led to increased cold susceptibility. Ptr ERF 109 overexpression caused extensive transcriptional reprogramming of several suites of stress‐responsive genes. Prx1 encoding class III peroxidase ( POD ) was one of the antioxidant genes exhibiting the greatest induction. Ptr ERF 109 was shown to directly bind to the promoter of PtrPrx1 (trifoliate orange Prx1 homologue) and positively activated its expression. In addition, the Ptr ERF 109 ‐overexpressing plants exhibited significantly higher POD activity and accumulated dramatically less H 2 O 2 and were more tolerant to oxidative stress, whereas the VIGS plants exhibited opposite trends, in comparison with wild type. Taken together, these results indicate that Ptr ERF 109 as a positive regulator contributes to imparting cold tolerance by, at least partly, directly regulating the POD ‐encoding gene to maintain a robust antioxidant capacity for effectively scavenging the reactive oxygen species. Our findings gain insight into better understanding of transcriptional regulation of antioxidant genes in response to cold stress.

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Section: Introductionmentioning

confidence: 99%

ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process

Wang

Dai

et al. 2019

Plant Biotechnology Journal

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“…The transcriptomic approach has revealed that plants deeply modify their transcriptomes in response to Cd stress [ 12 – 16 ] and these studies also indicated that transcriptional regulation of Cd tolerance-related genes is sometimes a conserved strategy among species. Previous studies have characterized several transcription factors (TFs) involved in Cd response [ 17 ] belonging to ERF, bZIP, WRKY, and bHLH TF families, supporting the complexity of plants response to Cd stress.…”

Section: Introductionmentioning

confidence: 99%

Activation of a gene network in durum wheat roots exposed to cadmium

et al. 2018

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BackgroundAmong cereals, durum wheat (Triticum turgidum L. subsp. durum) accumulates cadmium (Cd) at higher concentration if grown in Cd-polluted soils. Since cadmium accumulation is a risk for human health, the international trade organizations have limited the acceptable concentration of Cd in edible crops. Therefore, durum wheat cultivars accumulating low cadmium in grains should be preferred by farmers and consumers. To identify the response of durum wheat to the presence of Cd, the transcriptomes of roots and shoots of Creso and Svevo cultivars were sequenced after a 50-day exposure to 0.5 μM Cd in hydroponic solution.ResultsNo phytotoxic effects or biomass reduction was observed in Creso and Svevo plants at this Cd concentration. Despite this null effect, cadmium was accumulated in root tissues, in shoots and in grains suggesting a good cadmium translocation rate among tissues. The mRNA sequencing revealed a general transcriptome rearrangement after Cd treatment and more than 7000 genes were found differentially expressed in root and shoot tissues. Among these, the up-regulated genes in roots showed a clear correlation with cadmium uptake and detoxification. In particular, about three hundred genes were commonly up-regulated in Creso and Svevo roots suggesting a well defined molecular strategy characterized by the transcriptomic activation of several transcription factors mainly belonging to bHLH and WRKY families. bHLHs are probably the activators of the strong up-regulation of three NAS genes, responsible for the synthesis of the phytosiderophore nicotianamine (NA). Moreover, we found the overall up-regulation of the methionine salvage pathway that is tightly connected with NA synthesis and supply the S-adenosyl methionine necessary for NA biosynthesis. Finally, several vacuolar NA chelating heavy metal transporters were vigorously activated.ConclusionsIn conclusion, the exposure of durum wheat to cadmium activates in roots a complex gene network involved in cadmium translocation and detoxification from heavy metals. These findings are confident with a role of nicotianamine and methionine salvage pathway in the accumulation of cadmium in durum wheat.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1473-4) contains supplementary material, which is available to authorized users.

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“…). Considering that the induction is always a prediction for potential function (Wang et al , Yang et al , Zhao et al , Gao et al ) and upstream regulators such as the T. hispida eukaryotic translation initiation factor 1A ( TheIF1A ; Yang et al ), Phaseolus vulgaris Cd metal‐response element‐binding transcription factor 1 ( PvMTF‐1 ; Lin et al ), we can summarize that the drought‐inducing osmotic stress response of JrGSTTau1 is controlled by JrDREB2A , JrMYC2 , JrMYB44 , JrDof1 and JrWRKY7 , which may act as upstream regulators of JrGSTTau1 to either control JrGSTTau1 or act along with JrGSTTau1 to participate in plant drought stress response. Considering that the TFs of DREB, MYC, MYB, Dof and WRKY are members of a large family which have functions in plant osmotic stress responses, such as maize TaDREB3 (Shavrukov et al ), Tibetan hulless barley MYCs (Singh and Laxmi , Zeng et al ), Salicornia brachiata MYB44 (Shukla et al ), wheat TaMyb1D (Wei et al ), T. hispida Dof1.4 (Zang et al ), tomato SlCDF1‐5 (Corrales et al ), walnut JrWRKY7 and JrWRKY2 (Yang et al ).…”

Section: Discussionmentioning

confidence: 99%

“…Lin et al 2017), we can summarize that the drought-inducing osmotic stress response of JrGSTTau1 is controlled by JrDREB2A, JrMYC2, JrMYB44, JrDof1 and JrWRKY7, which may act as upstream regulators of JrGSTTau1 to either control JrGSTTau1 or act along with…”

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confidence: 92%

Multiple transcriptional regulation of walnut JrGSTTau1 gene in response to osmotic stress

Yang

Chen

et al. 2018

Physiologia Plantarum

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Glutathione S-transferases (GSTs) are important plant proteins involved in biotic and abiotic stress responses. A gene from Juglans regia, JrGSTTau1 was previously cloned and functionally characterized as an enzyme involved in improving cold tolerance in plants. To clarify the functional mechanism of JrGSTTau1 and its role in stress response, here, the JrGSTTau1 promoter including the up-stream regulators was examined using yeast one-hybrid together with transient expression assays, and the osmotic stress response ability was confirmed by comparing with wild-type plants. The 1500 bp JrGSTTau1 promoter displayed high GUS expression activity and was enhanced by mannitol stress. The promoter is composed of abundant cis-elements, some of which were osmotic stress response-related motifs, such as ABRE, DRE and MYB, indicating that the expression of JrGSTTau1 is regulated by potential up-stream regulators under abiotic stress. The transcription factors (TFs) of JrDREB2A, JrMYC2, JrMYB44, JrDof1 and JrWRKY7 were identified, which shared a similar response with JrGSTTau1 when exposed to PEG in walnut leaf and root. These results implied that JrDREB2A, JrMYC2, JrMYB44, JrDof1 and JrWRKY7 may act as up-stream regulators of JrGSTTau1 to regulate or combine functionality with JrGSTTau1 in osmotic stress response. Furthermore, compared with the WT plants, the transgenic tobacco plants that overexpress JrGSTTau1 showed improved tolerance to drought induced by osmotic stress, in which antioxidant enzymes, proline and reactive oxygen species (ROS) are involved. Our results demonstrated the positive role played by JrGSTTau1 in osmotic tolerance, which is regulated by multiple up-stream regulators.

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Transcription Factors PvERF15 and PvMTF-1 Form a Cadmium Stress Transcriptional Pathway

Cited by 65 publications

References 24 publications

ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process

ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process

Activation of a gene network in durum wheat roots exposed to cadmium

Multiple transcriptional regulation of walnut JrGSTTau1 gene in response to osmotic stress

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