Activation of a gene network in durum wheat roots exposed to cadmium

Aprile, Alessio; Sabella, Erika; Vergine, Marzia; Genga, A.; Siciliano, M.; Nutricati, Eliana; Rampino, Patrizia; Pascali, Mariarosaria De; Luvisi, Andrea; Miceli, Antonio; Negro, Carmine; Bellis, Luigi De

doi:10.1186/s12870-018-1473-4

Cited by 32 publications

(31 citation statements)

References 53 publications

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“…S3) (61). Also, in durum wheat root (Triticum turgidum), cadmium treatment induced contigs having the highest similarity to Arabidopsis TAT1 and TAT2 by 20 -50 and 3 times, respectively (62). Notably, TAT2 expression is 40 times higher than TAT1 in WT under normal light conditions (Fig.…”

Section: In Vivo Functions and Localization Of Arabidopsis Tat1 And Tat2mentioning

confidence: 92%

TAT1 and TAT2 tyrosine aminotransferases have both distinct and shared functions in tyrosine metabolism and degradation in Arabidopsis thaliana

Wang

Toda

Block

et al. 2019

Journal of Biological Chemistry

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Edited by F. Peter Guengerich Plants produce various L-tyrosine (Tyr)-derived compounds that are critical for plant adaptation and have pharmaceutical or nutritional importance for human health. Tyrosine aminotransferases (TATs) catalyze the reversible reaction between Tyr and 4-hydroxyphenylpyruvate (HPP), representing the entry point in plants for both biosynthesis of various natural products and Tyr degradation in the recycling of energy and nutrients. To better understand the roles of TATs and how Tyr is metabolized in planta, here we characterized single and double loss-of-function mutants of TAT1 (At5g53970) and TAT2 (At5g36160) in the model plant Arabidopsis thaliana. As reported previously, tat1 mutants exhibited elevated and decreased levels of Tyr and tocopherols, respectively. The tat2 mutation alone had no impact on Tyr and tocopherol levels, but a tat1 tat2 double mutant had increased Tyr accumulation and decreased tocopherol levels under highlight stress compared with the tat1 mutant. Relative to WT and the tat2 mutant, the tat1 mutant displayed increased vulnerability to continuous dark treatment, associated with an early drop in respiratory activity and sucrose depletion. During isotope-labeled Tyr feeding in the dark, we observed that the tat1 mutant exhibits much slower 13 C incorporation into tocopherols, fumarate, and other tricarboxylic acid (TCA) cycle intermediates than WT and the tat2 mutant. These results indicate that TAT1 and TAT2 function together in tocopherol biosynthesis, with TAT2 having a lesser role, and that TAT1 plays the major role in Tyr degradation in planta. Our study also highlights the importance of Tyr degradation under carbon starvation conditions during dark-induced senescence in plants.

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Section: In Vivo Functions and Localization Of Arabidopsis Tat1 And Tat2mentioning

confidence: 92%

TAT1 and TAT2 tyrosine aminotransferases have both distinct and shared functions in tyrosine metabolism and degradation in Arabidopsis thaliana

Wang

Toda

Block

et al. 2019

Journal of Biological Chemistry

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“…Aprile et al [54] suggested that wheat with low Cd and Pb in leaves has a high expression of the gene YSL2 which has a possible regulatory role in Cd compartmentalization in roots. The YSL2 is localized to vacuole membranes and can transport metal-NA complexes [55]. In addition, DiDonato Jr. et al [56] stated that YSL2 is located in many cell types in the roots and shoots, suggesting that diverse cell types gain metals as metal-NA complexes.…”

Section: Phytotoxicity and Detoxification Mechanism In Wheatmentioning

confidence: 99%

Cadmium Uptake by Wheat (Triticum aestivum L.): An Overview

Abedi

Mojiri

2020

Plants

116

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Cadmium is a toxic heavy metal that may be detected in soils and plants. Wheat, as a food consumed by 60% of the world’s population, may uptake a high quantity of Cd through its roots and translocate Cd to the shoots and grains thus posing risks to human health. Therefore, we tried to explore the journey of Cd in wheat via a review of several papers. Cadmium may reach the root cells by some transporters (such as zinc-regulated transporter/iron-regulated transporter-like protein, low-affinity calcium transporters, and natural resistance-associated macrophages), and some cation channels or Cd chelates via yellow stripe 1-like proteins. In addition, some of the effective factors regarding Cd uptake into wheat, such as pH, organic matter, cation exchange capacity (CEC), Fe and Mn oxide content, and soil texture (clay content), were investigated in this paper. Increasing Fe and Mn oxide content and clay minerals may decrease the Cd uptake by plants, whereas reducing pH and CEC may increase it. In addition, the feasibility of methods to diminish Cd accumulation in wheat was studied. Amongst agronomic approaches for decreasing the uptake of Cd by wheat, using organic amendments is most effective. Using biochar might reduce the Cd accumulation in wheat grains by up to 97.8%.

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“…The phylogeny of 136 WRKY proteins from the genus Glycyrrhiza displayed 22WRKYs (17GgWRKYs & 5GuWRKYs) belonging to group-I, 98 WRKYs (61 GgWRKYS & 37 GuWRKYs) clustering in group-II and 16 WRKY members comprising of group-III (4GgWRKYS & 12GuWRKYs). Group-II was further sub-divided into five sub-groups, IIa (11), IIb (17), IIc (16 + 8), IId (17), IIe (15) and an additional novel sub-group IIf (14) based on WRKY transcription factor rules adopted in Arabidopsis 9 . The present paper reports few exceptions observed in the WRKY members identified in the genus Glycyrrhiza.…”

Section: Phylogeny the Relatedness Among 136 Glycyrrhiza Wrky Proteimentioning

confidence: 99%

A Comprehensive Transcriptome-Wide Identification and Screening of WRKY Gene Family Engaged in Abiotic Stress in Glycyrrhiza glabra

Goyal

Manzoor

Vishwakarma

et al. 2020

Sci Rep

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The study reports 147 full-length WRKY genes based on the transcriptome analysis of Glycyrrhiza genus (G. glabra and G. uralensis). Additional motifs in G. glabra included DivIVA (GgWRKY20) and SerS Superfamily (GgWRKY21) at the C-terminal, and Coat family motifs (GgWRKY55) at the N-terminal of the proteins, while Exo70 exo cyst complex subunit of 338 amino acid (GuWRKY9) was present at the n-terminal of G. uralensis only. Plant Zn cluster super-family domain (17 WRKYs) and bZIP domain (2 WRKYs) were common between the two species. Based on the number of WRKY domains, sequence alignment and phylogenesis, the study identified GuWRKY27 comprising of 3 WRKY domains in G. uralensis and a new subgroup-IIf (10 members), having novel zinc finger pattern (C-X 4-C-X 22-HXH) in G. glabra. Multiple WRKY binding domains (1-11) were identified in the promoter regions of the GgWRKY genes indicating strong interacting network between the WRKY proteins. Tissue-specific expression of 25 GgWRKYs, under normal and treated conditions, revealed 11 of the 18 induction factor triggered response corroborating to response observed in AtWRKYs. The study identified auxin-responsive GgWRKY 55 & GgWRKY38; GA 3 responsive GgWRKYs15&59 in roots and GgWRKYs8, 20, 38, 57 &58 in the shoots of the treated plant. GgWRKYs induced under various stresses included GgWRKY33 (cold), GgWRKY4 (senescence), GgWRKYs2, 28 & 33 (salinity) and GgWRKY40 (wounding). Overall, 23 GgWRKYs responded to abiotic stress, and 17 WRKYs were induced by hormonal signals. Of them 13 WRKYs responded to both suggesting inter-connection between hormone signalling and stress response. The present study will help in understanding the transcriptional reprogramming, proteinprotein interaction and cross-regulation during stress and other physiological processes in the plant. The complexity in plant cell organization can be directly related to intricate inter-connections between genes and regulatory network inside the cell. This observation is further substantiated by studies on vast genomic and transcriptomic sequence information available in the public domain 1. The inter-cellular biological circuit in higher plants is governed at several discreet levels, one of them is regulated by a specific group of DNA binding proteins, the WRKYs 2 are among the ten largest families of transcription factors (TFs) in higher plants. The literature cites several papers after the first report on Ipomea batata (SPF1) in 1994 3 , from dicots 4 , monocots 5 , orchids 6 to unicellular eukaryote (Giardia lamblia) and the slime mold (Dictyostelium discoideum), revealing their evolutionary significance and complex organization 7,8. The 60 amino-acid characteristic conserved sequence of WRKY transcription factor (TFs) are most commonly identified by specific hepta-nucleotide signature sequence (WRKYGQK), the W-Box, which binds to the promoter sequence of target gene(s) modulating its activity 9. The large WRKY super-family is phylogenetically classified into three groups (I, II &III) based on the number of...

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Activation of a gene network in durum wheat roots exposed to cadmium

Cited by 32 publications

References 53 publications

TAT1 and TAT2 tyrosine aminotransferases have both distinct and shared functions in tyrosine metabolism and degradation in Arabidopsis thaliana

TAT1 and TAT2 tyrosine aminotransferases have both distinct and shared functions in tyrosine metabolism and degradation in Arabidopsis thaliana

Cadmium Uptake by Wheat (Triticum aestivum L.): An Overview

A Comprehensive Transcriptome-Wide Identification and Screening of WRKY Gene Family Engaged in Abiotic Stress in Glycyrrhiza glabra

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