Transcription Factor USF1 Is Required for Maintenance of Germline Stem Cells in Male Mice

Abstract

at the modification stage), and do not reflect editorial changes. No corrections/changes to the PDF manuscripts are accepted. Accordingly, there likely will be differences between the Advance Article manuscripts and the final, typeset articles. The manuscripts remain listed on the Advance Article page until the final, typeset articles are posted. At that point, the manuscripts are removed from the Advance Article page. DISCLAIMER: These manuscripts are provided "as is" without warranty of any kind, either express or particular purpose, or non-infringement. Changes will be made to these manuscripts before publication. Review and/or use or reliance on these materials is at the discretion and risk of the reader/user. In no event shall the Endocrine Society be liable for damages of any kind arising references to, products or publications do not imply endorsement of that product or publication.

Generation, localization and functions of macrophages during the development of testis

“…For seminiferous tubule whole-mount staining the testes of 7-days-, 2-, and 5-week-old mice were dissected and the tunica albuginea was removed. The seminiferous tubules were prepared for whole-mount stainings as previously described 60 . In brief, the samples were blocked (0.3% Triton X-100, 2% bovine serum albumin (BSA), and 10% fetal bovine serum (FBS)), and then sequentially incubated with primary unconjugated antibodies, secondary antibodies and directly conjugated antibodies diluted in appropriate blocking solutions.…”

Molecular regulation of spermatogonial stem cell renewal and differentiation

“…SSCs are specified perinatally as the population of fetal germ cells known as gonocytes or prospermatogonia migrates from the lumen of testis cords to the basement membrane. A subset of gonocytes contributes to the first wave of spermatogenesis, whereas the rest form the pool of SSCs (Mäkelä et al 2019). SSCs then actively proliferate to expand the population (Nagano et al 2000).…”

“…GDNF is indispensable for maintenance of SSCs both in vivo and in vitro (Meng et al 2000, Kubota et al 2004). Conversely, overexpression or increased availability of GDNF results in the accumulation of A undiff spermatogonia (Meng et al 2000, Uchida et al 2016, Masaki et al 2018a, Sharma & Braun 2018, Faisal et al 2019. GDNF acts on A undiff via binding to the GFRα1/RET receptor complex on their cell surface and subsequent activation of PI3K/AKT, RAS/ERK MAPK Figure 3 Regulation of A undiff in the adult mouse.…”

“…Whether it supports adoption of either the stem or progenitor state is unclear. Moreover, Wnt5a expression is downregulated in the testis soon after birth, and in the adult mouse Wnt5a levels are relatively low suggesting that it might be a developmental regulator of the SSC niche (Tanaka et al 2016, Faisal et al 2019.…”

“…Moreover, MIWI2, a protein associated with piRNA-mediated genome silencing and DNA methylation displays a restricted expression within progenitor A undiff , the significance of which is yet to be defined (Carrieri et al 2017, Vasiliauskaite et al 2018. Interestingly, the epigenome (DNA methylation at CpG sites plus histone modifications) of male germ cells undergoes profound changes during fetal development, whereas in postnatal germ cells, the epigenetic marks are more stable (Mäkelä et al 2019).…”