The involvement of bioactive factors in the self-renewal and stemness maintenance of spermatogonial stem cells

Yang, Guoqing; He, Yi; Yang, Hao

doi:10.1007/s11010-020-04028-7

Cited by 3 publications

(2 citation statements)

References 107 publications

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“…Meng et al 2000 displayed that seminiferous tubules are devoid of spermatogenic cells and Sertoli-cell-only phenotype observed in GDNF heterozygous null male mutant mice [ 18 ]. Also, previous studies showed that presence of GDNF in primary cultures of undifferentiated spermatogonia is required for the retention of the SSCs pool and regulation of self-renewal [ 19 – 21 ]. By microarray-based gene expression profiling, findings revealed that GDNF signaling up-regulated expression of self-renewal associated genes including Bcl6b, Lhx1 and Etv5 [ 1 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis

Salem,

Khadivi,

Javanbakht

et al. 2023

Stem Cell Res Ther

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The loss of germ cells and spermatogenic failure in non-obstructive azoospermia are believed to be the main causes of male infertility. Laboratory studies have used in vitro testicular models and different 3-dimensional (3D) culture systems for preservation, proliferation and differentiation of spermatogonial stem cells (SSCs) in recent decades. The establishment of testis-like structures would facilitate the study of drug and toxicity screening, pathological mechanisms and in vitro differentiation of SSCs which resulted in possible treatment of male infertility. The different culture systems using cellular aggregation with self-assembling capability, the use of different natural and synthetic biomaterials and various methods for scaffold fabrication provided a suitable 3D niche for testicular cells development. Recently, 3D culture models have noticeably used in research for their architectural and functional similarities to native microenvironment. In this review article, we briefly investigated the recent 3D culture systems that provided a suitable platform for male fertility preservation through organ culture of testis fragments, proliferation and differentiation of SSCs.

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Section: Introductionmentioning

confidence: 99%

Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis

Salem,

Khadivi,

Javanbakht

et al. 2023

Stem Cell Res Ther

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“…The self-renewal of SSCs without immortalization depends primarily on some cytokines, such as glial cell line-derived neurotrophic factor (GDNF), basic fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF), and epidermal growth factor (EGF) [ 12 , 13 ]. The neonatal mouse SSC line proliferates over 2 years with GDNF, bFGF, LIF, and EGF, and gives rise to the generation of offspring via transplantation into the recipient testis [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Spermatogonial Stem Cell Line with Potential of Meiosis in a Hermaphroditic Fish, Epinephelus coioides

Zhong

Tao

Liu

et al. 2022

Cells

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Spermatogonial stem cells (SSCs) are unique adult stem cells capable of self-renewal and differentiation into sperm. Grouper is a protogynous hermaphroditic fish farmed widely in the tropical and subtropical seas. In this study, we established an SSC line derived from adult testis of orange-spotted grouper, Epinephelus coioides. In the presence of basic fibroblast growth factor (bFGF) and leukemia inhibitory factor (LIF), the cells could be maintained with proliferation and self-renewal over 20 months and 120 passages under in vitro culture conditions. The cells exhibited strong alkaline phosphatase activity and the characteristics of SSCs with the expression of germ cell markers, including Vasa, Dazl, and Plzf, as well as the stem cell markers Nanog, Oct4, and Ssea1. Furthermore, the cultured cells could be induced by 11-ketotestosterone treatment to highly express the meiotic markers Rec8, Sycp3, and Dmc1, and produce some spherical cells, and even sperm-like cells with a tail. The findings of this study suggested that the cultured grouper SSC line would serve as an excellent tool to study the molecular mechanisms behind SSCs self-renewal and differentiation, meiosis during spermatogenesis, and sex reversal in hermaphroditic vertebrates. Moreover, this SSC line has great application value in grouper fish aquaculture, such as germ cell transplantation, genetic manipulation, and disease research.

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